Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Comparative Analysis of Microcystin during Water Treatment Process between Real-Time PCR and LC/MS

Real-Time PCR법과 LC/MS법을 이용한 수계중의 마이크로시스틴 검출방법 비교연구

  • Park, Hong-Gi (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Mi-Eun (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Cha, Dong-Jin (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Eun-Young (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Bean, Jae-Hoon (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
  • 박홍기 (부산광역시 상수도사업본부 수질연구소) ;
  • 정미은 (부산광역시 상수도사업본부 수질연구소) ;
  • 차동진 (부산광역시 상수도사업본부 수질연구소) ;
  • 정은영 (부산광역시 상수도사업본부 수질연구소) ;
  • 빈재훈 (부산광역시 상수도사업본부 수질연구소)
  • Received : 2010.04.05
  • Accepted : 2010.05.11
  • Published : 2010.08.30
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Abstract

We performed a comparative analysis using a Real-time PCR (Polymerase Chain Reaction) and LC/MS (Liquid-Chromatograph/Mass Spectrometer) method in order to detect microcystin in environmental sources. Among the three different primer sets tested for microcystin using three positive strains of Microcystis aeruginosa by Real-time PCR assay, only TOX2P/TOX2M primer pairs were able to detect Microcystis aeruginosa. According to the results of a survey carried out from June 2009 to September 2009, 11 out of 11 (100%) raw water samples were were found to have microcystin when the Real-Time PCR and LC/MS method was used, with total microcystin concentration ranging from 5.98~219.0 ${\mu}g/l$. A microcystin removal treatment process was used to ensure entire removal, by passing it through a BAC filtration step. It was concluded that real-time PCR assay can be used to estimate micrucystin detection more rapidly and easily than the LC/MS method.

현장의 환경 시료를 대상으로 Real-Time PCR법과 LC/MS법을 이용하여 마이크로시스틴 검출방법을 비교 연구하였다. 3종류의 primer쌍을 이용하여 3종의 Microcystis aeruginosa 표준균주를 대상으로 Real-Time PCR법을 실시한 결과 TOX2P/TOX2M primer를 이용한 균주에서만 마이크로시스틴이 검출되었다. 2009년 6~9월 사이에 남조류가 발생한 상수원수 시료를 정립된 Real-Time PCR법과 기존의 LC/MS법으로 실험한 결과 11개 시료 모두에서 마이크로시스틴이 검출되었고, 농도는 5.98~219.0 ${\mu}g/l$ 범위로 조사되었다. 정수처리 공정별 실험에서는 BAC 여과 단계에서 마이크로시스틴이 완전히 제거되는 것으로 나타났다. 실험결과 Real-Time PCR법은 기존의 표준시험방법인 LC/MS법 보다 분석시간을 많이 단축시키는 것으로 나타나 효과적인 마이크로시스틴 검출방법임을 알 수 있었다.

Keywords

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