Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Lipoxygenase Inhibitory and Antioxidant Activities of Isolated Compounds from Moutan Cortex

  • Ha, Do-Thi (College of Pharmacy, Chungnam National University) ;
  • Trung, Trinh-Nam (College of Pharmacy, Chungnam National University) ;
  • Thuan, Nguyen-Duy (Vietnam University of Traditional Pharmaco-Medicine) ;
  • Yim, Nam-Hui (College of Pharmacy, Chungnam National University) ;
  • Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
  • Received : 2010.02.15
  • Accepted : 2010.03.25
  • Published : 2010.06.30
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Abstract

Phytochemical investigation on the ethyl acetate and n-butanol fractions of Moutan Cortex resulted in the isolation and characterization of a new monoterpene glycoside (3) and twenty known monoterpene glycosides (1, 2, 4-21). The structure of 3 was determined by spectroscopic data interpretation and physico-chemical properties. Compounds 1 and 8 presented a remarkable inhibitory activity against lipoxygenase-1 (LOX-1) with $IC_{50}$ values of 45.2 and $37.5\;{\mu}M$, respectively. Compounds 9, 10, 13, 18, 19, and 21 showed significant 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect with $IC_{50}$ values of 9.8, 25.5, 6.4, 15.2, 18.7, and $23.7\;{\mu}M$, respectively. Benzoylpaeoniflorin (8), which exhibited the highest inhibitory effect with an $IC_{50}$ value of $37.5{\pm}0.7{\mu}M$, was further analyzed the inhibition kinetics by Lineweaver-Burk plots. Results indicated that 8 is a non-competitive inhibitor, and the kinetic parameter values were estimated to be ($31.04\;{\mu}M$, Ki), ($0.29\;{\mu}M/min$, $V_m$), and ($48.50\;{\mu}M$, $K_m$).

Keywords

References

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