The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Antioxidant and Hepatoprotective Effects of Amomum Xanthoides

  • Wang, Jing-Hua (Liver and Immunology Research Center, Institute of Traditional Medicine and Bioscience of Daejeon University) ;
  • Shin, Jang-Woo (Liver and Immunology Research Center, Institute of Traditional Medicine and Bioscience of Daejeon University) ;
  • Son, Chang-Gue (Liver and Immunology Research Center, Institute of Traditional Medicine and Bioscience of Daejeon University)
  • Received : 2010.03.05
  • Accepted : 2010.04.29
  • Published : 2010.05.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: To examine the in vitro antioxidant and hepatoprotective properties of Amomum xanthoides extract and its fractions. Methods: Methanol extract of Amomum xanthoides was fractionated into three fractions; petroleum ether fraction (PFAX), water fraction (WFAX) and ethyl acetate fraction (AFAX). The antioxidant and hepatoprotective effects of these fractions were compared by in vitro experiments. Results: The total phenols content and DPPH (1 1-Diphenyl-2-picrylhydrazyl) radical scavenging activity was found significant in AFAX when compared with other fractions (PFAX, WFAX). AFAX significantly inhibited NO production by RAW 264.7 cells in LPS-activated condition. In Hep $G_2$ cells, AFAX markedly prevented the leakage of LDH and release of AST against CCl4 challenge. Conclusion: Amomum xanthoides and especially ethyl acetate fraction have potent antioxidative and hepatoprotective properties.

Keywords

References

  1. Park BH, Park JW. The protective effect of Amomum xanthoides extract alloxan-induced diabetes through the suppression of NF-$\kappa$B activation. Exp Mol Med. 2001; 33:64-8. https://doi.org/10.1038/emm.2001.12
  2. Kwon KB, Kim JH, Lee YR, Lee HY, Jeong YJ, Rho HW, et al. Amomum xanthoides extract prevents cytokine-induced cell death of RINm5F cells through the inhibition of nitric oxide formation. Life Sci. 2003; 73:181-91. https://doi.org/10.1016/S0024-3205(03)00267-4
  3. Kim SH, Shin TY. Amomum xanthoides inhibits mast cell-mediated allergic reactions through the inhibition of histamine release and inflammatory cytokine production. Exp Biol Med. 2005; 230:681-7.
  4. Zhu JZ, Leng E, Chen DF, Zhang J. Effects of Amomum villosum on gastrointestinal motility and neurotransmitters in rats. Chinese Journal of Integrated Traditional and Western Medicine on Digestion. 2001; 9(4):205-7.
  5. Feng GW, Tao L, Shen XC, Hao M, Peng J. Effect of Amomum villosum extraction on the contraction of rabbit thoracic aorta in vitro. Lishizhen Medicine and Materia Medica Research. 2006; 17(11):2223-5.
  6. Wu X, Li X, Xiao F, Zhang Z, Xu Z, Wang H. Studies on the analgesic and anti-inflammatory effect of bornyl acetate in volatile oil from Amomum villosum. Zhong Yao Cai. 2004; 27(6):438-9.
  7. Hu YL, Zhang ZY, Lin JM. Advances in study on chemical constituents and pharmacological activities of Amomum xanthoides. Zhong Yao Cai. 2005; 28(1):72-4.
  8. Sun L, Yu JG, Zhou LD, Luo XZ, Din W, Yang SL. Two flavone glycosides from Chinese traditional medicine Amomum villosum. Zhongguo Zhong Yao Za Zhi. 2002; 27(1):36-8
  9. Hu XP, Shin JW, Wang JH, Cho JH, Son JY, Cho CK, et al. Antioxidative and hepatoprotective effect of CGX, an herbal medicine, against toxic acute injury in mice. J Ethnopharmacol. 2008; 120:51-5. https://doi.org/10.1016/j.jep.2008.07.042
  10. Sadasivam S, Manikam A. Biochemical methods for agricultural sciences. Wiley Eastern Limited, New Delhi, p.187, 1992.
  11. Oki T, Masuda M, Kobayashi M, Nishiba Y, Furuta S, Suda I, et al. Polymeric procyanidins as radical-scavenging components in red-hulled rice. J Agric Food Chem. 2002; 50(26):7524-9. https://doi.org/10.1021/jf025841z
  12. Griess P. Bemerkungen zu der abhandlung der H.H. Weselsky und Benedikt "Ueber einige azoverbindungen." Chem. Ber. 1879; 12:426-8. https://doi.org/10.1002/cber.187901201117
  13. Cho JH, Lee YY, Seo SH, Yoo HS, Choi WJ, Lee YW, et al. A clinical report about 57 patients with chronic liver disease. J Korean Oriental Med. 2001; 21:112-21.
  14. Son CG, Choi BL, Shin JW, Choi WJ, Cho JH, Song KC. Effect of Gamichunggantang on alcoholic metabolism and alcoholic liver disease. J Korean Oriental Med. 2001; 2:89-98.
  15. Son CG, Choi WJ, Shin JW, Han SH, Cho JH, Song KC, et al. Effects of gamichunggantang on hyperlipidemia. Acta Pharmacologica Sinica. 2003; 24:133-9.
  16. Shin JW, Son JY, Oh SM, Han SH, Wang JH, Cho JH, et al. An herbal formula, CGX, exerts hepatotherapeutic effects on dimethylnitrosamineinduced chronic liver injury model in rats. World J Gastroenterol. 2006; 12(38):6142-8.
  17. Wang JH, Shin JW, Son JY, Cho JH, Son CG. Antifibrotic effects of CGX, a traditional herbal formula, and its mechanisms in rats. J Ethnopharmacol. 2010; 127:534-42. https://doi.org/10.1016/j.jep.2009.10.001
  18. Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal plant extracts for antioxidant capacity and phenols. Food Chem. 2006; 94:550-7. https://doi.org/10.1016/j.foodchem.2004.12.004
  19. Sawadogo WR, Meda A, Lamien CE, Kiendrebeogo M, Guissou IP, Nacoulma OG. Phenolic content and antioxidant activity of six acanthaceae from Burkina Faso. Aust J Biol Sci. 2006; 6:249-52. https://doi.org/10.3923/jbs.2006.249.252
  20. Surveswaran S, Cai YZ, Corke H, Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem. 2007; 102:938-53. https://doi.org/10.1016/j.foodchem.2006.06.033
  21. Shaker E, Mahmoud H, Mnaa S. Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem Toxicol. 2010; 48(3):803-6. https://doi.org/10.1016/j.fct.2009.12.011
  22. Hatano M, Sasaki S, Ohata S, Shiratsuchi Y, Yamazaki T, Nagata K, et al. Effects of Kupffer cell-depletion on Concanavalin A-induced hepatitis. Cell Immunol. 2008; 251(1):25-30. https://doi.org/10.1016/j.cellimm.2008.02.003
  23. Plaa GL. Chlorinated methanes and liver injury: highlights of the past 50 years. Annu Rev Pharmacol Toxicol. 2000; 40:42-65.
  24. Parola MG, Leonarduzzi F, Biasi E, Albano ME, Biocca G, Poli MU, et al. Vitamin E dietary supplementation protects against CC$L_{4}$-induced chronic liver damage and cirrhosis. Hepatology. 1992; 16:1014-21. https://doi.org/10.1002/hep.1840160426