The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Deodorizing Effect of Several Antibacterial Medicinal Herbs on Oral Malodor

항균작용을 가진 수종 한약재의 구취억제 효과

  • Kim, Hyun-Kyung (School of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Jae-Woo (School of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Yoon, Seong-Woo (School of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Ryu, Bong-Ha (School of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Jin-Sung (School of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 김현경 (경희대학교 한의과대학 비계내과학교실) ;
  • 박재우 (경희대학교 한의과대학 비계내과학교실) ;
  • 윤성우 (경희대학교 한의과대학 비계내과학교실) ;
  • 류봉하 (경희대학교 한의과대학 비계내과학교실) ;
  • 김진성 (경희대학교 한의과대학 비계내과학교실)
  • Received : 2010.04.28
  • Accepted : 2010.07.12
  • Published : 2010.07.31
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Abstract

Objective: We investigated the oral malodor inhibitory effect of Scutellariae Radix (SR), Phellodendri Cortex (PC), Moutan Cortex (MTC) and Magnoliae Cortex (MGC) for the development of a gargle solution. Methods: 1. Against P. gingivalis and Pr. intermedia, the minimal bactericidal concentration (MBC) and the change of viable cells that were exposed to 1% each herbal extract were observed. 2. Deodorizing activity of 2% herbal extract and Garglin $Mint^{(R)}$ against methyl mercaptan were evaluated by gas chromatography (GC). 3. We used the salivary sediment system (SSS) as the malodor model. 4. The clinical examination was repeated 3 times by 2 subjects by $Halimeter^{(R)}$. Baseline VSC of each subject was measured. Then, the control subject gargled with cysteine for 30 sec. After 4 min, subjects would gargle for 30 seconds with herbal extracts (2%) and Garglin $Mint^{(R)}$. Subsequently, the concentration of VSC was measured at 0, 4, 8, 12, 16, 20, 40 and 60 minutes. Results: 1. Against P. gingivalis, MBC of SR, PC and MTC was 0.1%, and MBC of MGC was 1%. Removal time of P. gingivalis was as follows; 5 hr in MGC, 24 hr in SR and PC, and 48 hr in MTC. Against Pr. intermedia, MBC of SR and PC was 0.5%, and MBC of MTC, MGC was 1%. Removal time of Pr. intermedia was as follows; 5 hr in MTC and 24 hr in SR, PC and MGC. 2. Deodorizing effect of herbal extracts against methyl mercaptan was as follows; MGC and MTC had 100%, SR had 82.22%, PC had 66.60%, Garglin $Mint^{(R)}$ had 40.54%. 3. In the experiment using SSS, PC and MTC had statistically significant malodor-inhibitory effects (p<.05). 4. In the clinical examination, PC and MGC had statistically significant inhibitory effects at every elapsed time compared to the control subject. MTC had that until 40 min. SR had that at 0, 4, 8, 20, and 60 min. Conclusions: SR, PC, MTC and MGC have an antibacterial effect and the chemical removable activity of the oral malodor caused by VSC. These four herbs could have potential as effective anti-malodor agents.

Keywords

References

  1. Tonzetich J. Prodcution and origin of oral malodor: a review of mechanisms and methods of analysis. J Periodontol. 1977;48(1):13-20. https://doi.org/10.1902/jop.1977.48.1.13
  2. Feller L, Blignaut E. Halitosis: a review. SADJ. 2005;60(1):17-9.
  3. Yang SJ, Moon HS, Kim JB. A study on the effect of toothbrushing and tongue-brushing in reduction of oral malodor. J korean Acad Dent Health. 1993;17(2):268-278.
  4. Kang EH, Lim HS, Kim DK, Seong JH. Effectiveness of toothpick method on the reduction of oral malodor. J korean Acad Dent Health. 2004;8(1):127-138.
  5. Park JH, Han KS, Kim MK. Effect of tongue scraping, ZnCl2 mouth rinse, and periodontal treatment on the reduction of oral malodor. Kor J Oral Med. 2000;25(1):41-51.
  6. Flotra L. Side effects of chlorohexidine mouth washes. Scan J Dent Res. 1971;79:119-25.
  7. Park JB, Auh QS, Chun YH, Lee JY, Hong JP. The effect of the Phytoncide in Decreasing the Mouth Odor. J Kor Acad Oral Med. 2007;32(2):151-156.
  8. Lee DS, Kim SB, Kim TJ, Kim JH, Ji CI, Park JH. Deodorant effect of marine algae extracts on halitosis. Bull Nat'l Fish Res Dev Inst Korea. 1999;57:195-201.
  9. Lee JH, Kim ME, Kim SB. Study on the development of gargle solution containing medicinal herb extract for oral malodor. Kor J Oral Med. 2003;28(1):1-10.
  10. Eom GH, Kim JS. Deodorizing effect of Cheonggugamrosu. Kor J Orient Int Med. 2007;28(2):354-62.
  11. Kim JS, Park JW, Yoon SW, Ryu BH. Inhibitory effect of respective herbs in Cheonggugamrosu on oral malodor using modeling of the Salivary Sediment System. J Korean Oriental Med. 2009;30(2):79-87.
  12. Park SK, Hong SS, Lim JH, Han SY, Ryu JM, Jang SY et al. Effect of Junghyanghwan on reducing halitosis and the comparison with GARGLIN and Normal Saline. Kor J Orient Int Med. 2003;spr(1):154-68.
  13. De Boever EH, Loesche WJ. Assessing the contribution of anaerobic microflora of the tongue to oral malodor. JADA. 1995;126:1384-93.
  14. Tokita F, Ishikawa M, Shibuya K, Koshimizu M, Abe R. Deodorizing activity of some plant extracts against methyl mercaptan. Nippon Nogeikagaku Kaishi. 1984;58(6):585-9. https://doi.org/10.1271/nogeikagaku1924.58.585
  15. Kleinberg I, Codipilly D. $H_{2}S$ generation and Eh reduction in cysteine challenge testing as a means of determining the potential of test products and treatment for inhibiting oral malodor. J Breath Res. 2008; 2(1):1-9.
  16. Delanghe G, Ghyselen J, Van Steenberghe D, Feenstra L. Multidisciplinary breath-odour clinic. Lancet. 1997;350(9072):187.
  17. Parth L, Ken Y, Ali K, Toshio I, Tsutomu S, Tomoko T et al. Effect of Green Tea on Volatile Sulfur Compounds in Mouth Air. J Nutr Sci Vitaminol. 2008;54:89-94. https://doi.org/10.3177/jnsv.54.89
  18. Wang L. The effect of radix scutellariae and Tea Polyphenol on halitosis-correlated bacteria and substrate. 四川大學化西口腔醫學院碩士學位論文. 2005.
  19. Wang L, Yang XZ, HU DY. Anti-halitosis effect of radix scutellariae and tea polyphenol. Chin J Conserv Dent. 2006;16(3):149-52.
  20. 전국한의과대학 공동교재편찬위원회 공저. 본초학. 서울: 영림사; 2006, 218-20, 223-5, 237-8, 336-7.
  21. 김호철. 한약약리학. 서울: 집문당; 2001, 129-33, 138-40, 147-50, 211-3.
  22. 謝龍. 中藥治療口臭臨床觀察. 實用中西醫結合雜誌. 1997;10(17):1723.
  23. 曾海付燦鋆. 甘露飮加味治療구취 80例. 實用中醫內科雜誌. 2005;19(5);467.
  24. ZHU CL, LIM IY. Screenig of chinese herbs for anit-halitosis activity. Acd J Shanghai Sec Med Univ. 2005;25(4):345-348.
  25. Chung YG, Kwon OJ. Anibacterial action of water extracts of Korean medicinal herbs. J Kor Env Hygi Sci. 1993;3(1):233-239.
  26. Cho SH, Kim YR. Antimicrobial Characteristics of Scutellariae Radix Extract. J Kor Soc Food Sci Nutr. 2001;30(5);964-968.
  27. Son DH, Lee SI, Chung YG. Antioxidative of medicinal plants on pathogenic bacteria. J Kor Soc Hygi Sci. 2001;7(2):103-108.
  28. Park UY, Chang DS, Cho HR. Screening of Antimicrobial activity for medicinal herb extracts. J Kor Sco Food Nutr. 1992;21(1):91-96.
  29. Cai H. Choi SI, Lee YM, Hoe TR. Antimicrobial effects of herbal medicine extracts on Staphylococcus aureus and Escherichia coli O157:H7. Kor J Biotechnol Bioeng. 2002;17(6):537-542.
  30. Kweon OG, Son JC, Kim SC, Chung SK, Park SW. antimicrobial and antioxidative activities from Moutan Cortex Extract. Kor J Postharvest Sci Technol. 1998;5(3):281-285.
  31. Bae KH, Oh HR. Synergistic effect of lysozyme on bacterical activity of magnolol and honokiol against a carriogenic bacterium, Streptococcus mutans OMZ 176. Arch Pharm Res. 1990;13:117-119. https://doi.org/10.1007/BF02857847
  32. Chung CP, Ku Y, Bae KH. Biological effect of magnolia and Gingo Biloba extract to the antimicrobial antiinflammatory and cellular activity. J Kor Acad Periodontol. 1995;25(3): 478-486.
  33. Greenberg M, Umezis P, Tian M. Compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor. J Agric Food Chem. 2007;55(23):9465-9. https://doi.org/10.1021/jf072122h
  34. 홍정표. 구취. 경희의학. 2000;16(1):4-7.
  35. Hunter CM, Niles HP, Vazquez J, Kloos C, Subramanyam R, Williams MI et al. Breath odor evaluation by detection of volatile sulfur compounds- correlation with organoleptic odor ratings. Oral Dis. 2005;11 Suppl 1:48-50.
  36. Baharvand M, Maleki Z, Mohammadi S, Alavi K, Moghaddam EJ. Assessment of oral malodor: a comparison of the organoleptic method with sulfide monitoring. J Contemp Dent Pract. 2008 Jul 1;9(5):76-83.
  37. Kleinberg I, Codipilly M. Modeling of the oral malodor system and methods of analysis. Quintessence Int. 1999;30:357-69.
  38. Nakano Y, Yoshimura M, Koga T. Correlation between oral malodor and periodontal bacteria. Microbes Infect. 2002;4:679-683. https://doi.org/10.1016/S1286-4579(02)01586-1
  39. Mel Rogenberg. 구취: 진단 및 연구방법. 서울:신흥인터내셔널; 1998.