Effect of YCT on Insulin Secretion in RIN-m5F Cells

RIN-m5F 세포에서 야관청혈탕(夜關淸血湯)이 인슐린 분비에 미치는 영향

  • Kim, Jin-Mi (Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Cho, Chung-Sik (Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University) ;
  • Kim, Chul-Jung (Dept. of Internal Medicine, College of Oriental Medicine, Dae-jeon University)
  • 김진미 (대전대학교 한의과대학 신계내과학교실) ;
  • 조충식 (대전대학교 한의과대학 신계내과학교실) ;
  • 김철중 (대전대학교 한의과대학 신계내과학교실)
  • Received : 2010.03.02
  • Accepted : 2010.05.28
  • Published : 2010.07.31

Abstract

Objective: This study was performed to investigate the effect of Yagwan-cheunghyeoltang (YCT) on insulin secretion in RIN-m5F cells. Methods: After treatment with various concentrations of YCT to RIN-m5F cells, cell viability, free radical-scavenging activity, SOD activity, and insulin secretion were measured. Additionally, insulin-related gene expressions were measured using real-time RT-PCR. Results: 1. YCT didn't show any influence on RIN-m5F cells viability. 2. YCT showed free radical-scavenging activity by 16% at $100{\mu}g/m{\ell}$ of concentration. 3. YCT showed enhancement of SOD activity by 60% at $100{\mu}g/m{\ell}$ of concentration. 4. YCT significantly increased insulin secretion in RIN-m5F cells in a dose-dependent manner. 5. YCT up-regulated INS-1, INS-2, IRS-1, IRS-2 and IRS-3 mRNA expressions compared to the control group. 6. YCT down-regulated INS-R, GCK, GLP-1R and GLP-2R mRNA expressions compared to the control group. Conclusion: YCT has pharmaceutical properties enhancing insulin production and controlling glucose-associated metabolism, and could be a candidate for drug development after further research.

Keywords

References

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