Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Antimicrobial Activity of Korean Herbal Complex Extract and Clay Mineral Mixture against Escherichia coli O157:H7

한약재 복합추출물과 점토 광물질 혼합제의 Escherichio coli O157:H7에 대한 항균효과

  • Lee, Yeon-Ok (Senong Co., Ltd.) ;
  • Jung, Won-Chul (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Kim, Gon-Sup (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Yeo-Eun (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University) ;
  • Kim, Suk (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • 이연욱 (주식회사 세농) ;
  • 정원철 (경상대학교 수의과대학 생명과학연구소) ;
  • 차춘남 (경상대학교 산업시스템공학부 공학연구원) ;
  • 김곤섭 (경상대학교 수의과대학 생명과학연구소) ;
  • 이여은 (경상대학교 보건대학원 환경보건학과) ;
  • 김석 (경상대학교 수의과대학 생명과학연구소) ;
  • 이후장 (경상대학교 수의과대학 생명과학연구소)
  • Received : 2010.01.08
  • Accepted : 2010.01.18
  • Published : 2010.03.31
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Abstract

The present study was evaluated the antibacterial effect of the combination of Coptidis rhizoma, Lonicerae Flos, and Paeonia japonica (1:1:1) extracts (CLP1000). Also, the effectiveness of CLP1000, dioctahedral smectite (DHS), and the combination of CLP1000 and DHS (CLPS1000) against E. coli O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 10% and 20% CLP1000 were inhibited the growth of E. coli O157:H7 by 30% and 47%, respectively. For 7 days after single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were orally administered with saline, 10% CLP1000, 10% DHS, and 10% CLPS1000, respectively. On the 3rd day, the number of E. coli O157:H7 in mouse feces was significantly decreased by administration of CLP1000 (p < 0.05), DHS (p < 0.05) and CLPS1000 (p < 0.001). On the 7th day, CLP1000 (p < 0.05) and CLPS1000 p < 0.001) administration significantly decreased the number of E. coli O157:H7. According to the results of the present study, administration of CLPS1000 to mice can reduce the severity of E. coli O157:H7 infection. Also, it is suggested that CLPS100 represents a good candidate for the treatment of enteric infections in domestic animals.

본 연구는 황련, 금은화, 그리고 백작약 등 한약재 복합 추출물의 E. coli O157:H7에 대한 항균효과와, 한약재 복합추출물과 dioctahedral smectite의 합제의 E. coli O157:H7 감염 마우스에 대한 효과를 평가하기 위해 수행되었다. 한약재 복합추출물, CLP1000을 이용하여 E. coli O157:H7에 대한 항균효과 확인 시험을 수행한 결과, CLP1000 10%와 20%를 첨가한 배지에서 E. coli O157:H7의 성장을 각각 30%와 47%를 억제하는 효과가 관찰되었다. 또한, E. coli O157:H7을 김염시킨 마우스에 CLP1000, dioctahedral smectite (DHS), 그리고 CLP1000와 DHS 합제 (CLPS1000)를 각각 7 일 동안 경시별로 마우스 분변 중의 E. coli O157:H7 균수의 변화를 관찰한 결과, 투여 3일째에, 10% CLPS1000을 투여한 군에서 대조군과 비교하여 E. coli O157:H7의 균수가 유의성 있게 감소하였으며(p < 0.05), 투여 7일째에는 10% CLP1000 (p < 0.05)과 10% CLPS1000을 투여한 군에서 대조군과 비교하여 E. coli O157:H7의 균수가 유의성 있게 감소하는 결과를 나타내었다. 따라서 본 연구의 결과로부터, CLPS1000을 E. coli O157:H7에 감염된 마우스에 투여할 경우 감염증상을 완화 시킬 수 있을 것으로 기대된다.

Keywords

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