Evaluation of Antibacterial Effects of a Combination of Coptidis Rhizoma, Lonicerae Flos, Paeonia Japonica Extracts, and Dioctahedral Smectite Against Salmonella Typhimurium in Murine Salmonellosis

  • Jung, Won-Chul (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Yeon-Ok (Senong Co., Ltd.) ;
  • Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Lee, Yeo-Eun (Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University) ;
  • Kim, Gon-Sup (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Live Sciences, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2010.01.02
  • Accepted : 2010.01.27
  • Published : 2010.03.31

Abstract

The present study was undertaken to estimate the antibacterial effect of a combination of C. rhizoma, L. Flos, and P japonica (1:1:1) extracts (CLP1000) and a combination of the herbal extract mixture and dioctahedral smectite (CLPS1000) against murine salmonellosis. At the concentration of CLP1000 and CLPS1000 0.5 mg/ml, the antibacterial effect was not showed on Salmonella typhimurium (S. typhimurium). On the other hand, the antibacterial effect against S. typhimurium was observed at the concentration of CLP1000 and CLPS1000 1.0 mg/ml. Oral administration of Smectite, CLPl000, and CLPS1000 at the dose of 10 mg/ml showed a therapeutic effect for S. typhimurium infected BALB/c mice. The mortality of Smectite, CLP1000 and CLPS1000-treated mice was 90%, 90%, and 70% at 12 days, respectively, while that of untreated mice was 100% at 9 days after a lethal dose of S. typhimurium infection. The results of our study strongly indicate that CLPS1000 has potential as an effective of salmonellosis.

본 연구는 황련, 금은화, 그리고 백작약 혼합분말(1:1:1)의 메탄올 추출물(CLP1000)과, 이에 dioctahedral smectite를 혼합한 합제 (CLPS1000)의 살모넬라에 대한 항균효과를 평가하기 위해 수행되었다. CLP1000와 CLPS1000는, 0. 5mg/ml 농도에서 S. typhimurium에 대한 항균효과를 보이지 않았으나, 1.0 mg/ml의 농도에서는 S. typhimurium에 대한 항균활성이 관찰되었다. BALB/c 마우스를 이용한 살모넬라 감염시험에서 Smectite, CLP1000 그리고 CLPS1000을 각각 10 mg/ml 농도로 12일 동안 투여한 결과, 사망률이 각각 90%, 90% 그리고 70%을 보여, CLPS1000이 마우스 살모넬라증에 강력한 효과를 갖고 있는 것으로 나타났다.

Keywords

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