Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Cellulose Hydrolysis by Digestive Enzymes of Reticulitermes speratus, a Native Termite from Korea

  • Lee, Young-Min (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Kim, Hyun-Jung (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Cho, Moon-Jung (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Shin, Keum (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Kim, Young-Kyoon (Department of Forest Products, College of Forest Science, Kookmin University) ;
  • Kim, Yeong-Suk (Department of Forest Products, College of Forest Science, Kookmin University)
  • Received : 2010.03.03
  • Accepted : 2010.03.08
  • Published : 2010.03.25
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Abstract

This study was to investigate the enzymatic hydrolysis of cellulose using the cellulase from whole body of the native termite collected in Milyang-si, Kyungsangnamdo, Korea. In the results, optimal temperature and pH for the enzyme of native termites were $45^{\circ}C$ and pH 5.5 for both endo-${\beta}$-1, 4-glucanase and ${\beta}$-glucosidase. Enzyme activity of the termite enzyme was shown $8.8{\times}10^{-2}\;FPU/m{\ell}$. And the highest glucose hydrolysis rate of cellulose by the digestive enzyme from test termites was 24.5% based on the glucan, comparing 59.7% by commercial enzyme (only celluclast 1.5 L) at 1% (w/v) substrate and 36 hours in hydrolysis time. This hydrolysis rate by the digestive enzyme from test termites was comparatively high value in 41% level of the commercial enzyme. When cellulose was hydrolyzed by the digestive enzyme of the native termite, glucose hydrolysis was almost completed in 12 hours which was the considerably reduced time for cellulose hydrolysis. It was suggested that the quiet short reaction time for cellulose hydrolysis by the enzyme from native termite could be a very high advantage for development of hydrolysis cellulase for lignocellulosic biomass.

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References

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