Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
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Genetic Stability Studies in Micropropagated Date Palm (Phoenix dactylifera L.) Plants using Microsatellite Marker

  • Kumar, Nitish (Plant Tissue Culture Laboratory, Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Singh, Amritpal S. (Plant Tissue Culture Laboratory, Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Modi, Arpan R. (Plant Tissue Culture Laboratory, Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Patel, Armi R. (Plant Tissue Culture Laboratory, Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Gajera, Bhavesh B. (Plant Tissue Culture Laboratory, Department of Agricultural Biotechnology, Anand Agricultural University) ;
  • Subhash, Narayanan (Plant Tissue Culture Laboratory, Department of Agricultural Biotechnology, Anand Agricultural University)
  • Received : 2009.03.12
  • Accepted : 2010.04.29
  • Published : 2010.04.30
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Abstract

Sixteen microsatellite markers (simple sequence repeat (SSR) markers) were employed to examine the genetic stability of 27 randomly chosen date palm (Phoenix dactylifera L.) plants produced through somatic embryogenesis with upto forty two in vitro subcultures. No microsatellite DNA variation was observed among all micropropagated plants. Our results indicate that the micropropagation protocol used for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated that somatic embryogenesis can also be used as one of the safe modes for production of true-to-type plants of date palm. This is the first report on the use of microsatellite DNA markers to establish the genetic stability in micropropagated date palm plants.

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References

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