Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Skin Aging Effect of Syriacusins from Hibiscus Syriacus on Ultraviolet-Irradiated Human Dermal Fibroblast Cells

  • Ryoo, In-Ja (Korea Research Institute of Bioscience and Biotechnology) ;
  • Moon, Eun-Yi (Department of Bioscience and Biotechnology, Sejong University) ;
  • Kim, Young-Hee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Ik-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Choo, Soo-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2010.05.07
  • Accepted : 2010.05.21
  • Published : 2010.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

Photosensitized peroxidation of membrane lipids has been implicated in skin pathologies such as phototoxicity and premature aging. We have previously reported that syriacusin compounds isolated from Hibiscus Syriacus inhibited lipid peroxidation. Here, we investigated whether syriacusins could be effective inhibitor to skin aging using ultraviolet-irradiated human dermal fibroblast cells (HDFCs). Syriacusins A, B, and C inhibit the activity of human neutrophil elastase (HNE), a serine protease to degrade extracellular matrix (ECM) proteins including elastin, with $IC_{50}s$ of 8.0, 5.2, and $6.1\;{\mu}M$, respectively. No changes in cell viability were detected by syriacusins A and B in UV-B ($10\;mJ/cm^2$) irradiated HDFCs. Matrix metallo-proteinase (MMP)-1 expression in HDFCs was increased by UV-B irradiation. MMP-1 expression in UV-B irradiated HDFCs was decreased by $10\;{\mu}M$ and $20\;{\mu}M$ syriacusin A to 50% and 20% of untreated control, respectively. Syriacusin B treated with $20\;{\mu}M$ reduced MMP-1 expression in UV-B irradiated HDFCs to 60% of untreated control. Syriacusin A also inhibited MMP-2 expression accompanying the increase of type-I pro-collagen in UV-B irradiated HDFCs. These results demonstrate that syriacusin A could be a more effective compound to inhibit skin aging caused by UV irradiation. It suggests that syriacusins A and B might be developed as possible agents to treat or prevent skin aging.

Keywords

References

  1. Amemiya, T., Sasamura, H., Mifune, M., Kitamura, Y., Hirahashi, J., Hayashi, M. and Saruta, T. (1999). Vascular endothelial growth factor activates MAP kinase and enhances collagen synthesis in human mesangial cells. Kidney Int. 56, 2055-2063. https://doi.org/10.1046/j.1523-1755.1999.00796.x
  2. Antonicelli, F., Bellon, G., Debelle, L. and Hornebeck, W. (2007).Elastin-elastases and inflamm-aging. Curr. Top. Dev. Biol.79, 99-155. https://doi.org/10.1016/S0070-2153(06)79005-6
  3. Bernstein, E. F., Chen, Y. Q., Tamai, K., Shepley, K. J., Resnik,K. S., Zhang, H., Tuan, R., Mauviel, A. and Uitto, J. (1994).Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J. Invest. Dermatol. 103, 182-186. https://doi.org/10.1111/1523-1747.ep12392693
  4. Choi, W. S., Mitsumoto, A. and Kochevar, I. E. (2009). Involvement of reactive oxygen species in TGF-beta1-induced tropoelastin expression by human dermal fibroblasts. Photochem. Photobiol. 85, 1425-1433. https://doi.org/10.1111/j.1751-1097.2009.00611.x
  5. Cortez, D. M., Feldman, M. D., Mummidi, S., Valente, A. J., Steffensen, B., Vincenti, M., Barnes, J. L. and Chandrasekar, B. (2007). IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta, NF-kappaB, and AP-1 activation. Am. J. Physiol. Heart Circ. Physiol. 293, H3356-3365. https://doi.org/10.1152/ajpheart.00928.2007
  6. Demeule, M., Brossard, M., Page, M., Gingras, D. and Beliveau,R. (2000). Matrix metalloproteinase inhibition by green tea catechins. Biochim. Biophys. Acta. 1478, 51-60. https://doi.org/10.1016/S0167-4838(00)00009-1
  7. Denizot, F. and Lang, R. (1986). Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods. 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  8. Di Girolamo, N., Coroneo, M. T. and Wakefield, D. (2003). UVBelicited induction of MMP-1 expression in human ocular surface epithelial cells is mediated through the ERK1/2 MAPK-dependent pathway. Invest. Ophthalmol. Vis. Sci. 44,4705-4714. https://doi.org/10.1167/iovs.03-0356
  9. Dong, K. K., Damaghi, N., Picart, S. D., Markova, N. G., Obayashi,K., Okano, Y., Masaki, H., Grether-Beck, S., Krutmann, J., Smiles, K. A. and Yarosh, D. B. (2008). UV-induced DNA damage initiates release of MMP-1 in human skin. Exp. Dermatol. 17, 1037-1044. https://doi.org/10.1111/j.1600-0625.2008.00747.x
  10. Fagot, D., Asselineau, D. and Bernerd, F. (2002). Direct role of human dermal fibroblasts and indirect participation of epidermal keratinocytes in MMP-1 production after UV-B irradiation. Arch. Dermatol. Res. 293, 576-583. https://doi.org/10.1007/s00403-001-0271-1
  11. Fagot, D., Asselineau, D. and Bernerd, F. (2004). Matrix metalloproteinase-1 production observed after solar-simulated radiation exposure is assumed by dermal fibroblasts but involves a paracrine activation through epidermal keratinocytes. Photochem. Photobiol. 79, 499-505. https://doi.org/10.1562/YG-03-11-R1.1
  12. Girotti, A. W. and Kriska, T. (2004). Role of lipid hydroperoxides in photo-oxidative stress signaling. Antioxid. Redox. Signal.6, 301-310. https://doi.org/10.1089/152308604322899369
  13. Hsu, H. Y., Chen, Y. P., Shen, S. J., Hsu, C. S., Chen, C. C. andChang, H. C. (1986). Oriental Material Medica, a Concise Guide, pp. 503-504. Healing Arts Institute, Taipei.
  14. Huang, K. C., (1993). The Pharmacology of Chinese Herbs, pp. 193-194. CRC Press, Tokyo.
  15. Imokawa, G. (2008). Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: a pivotal role of fibrobrast-derived elastase. Arch. Dermatol. Res. 300(Suppl 1), S7-20. https://doi.org/10.1007/s00403-007-0798-x
  16. Imokawa, G. (2009). Mechanism of UVB-induced wrinkling of the skin: paracrine cytokine linkage between keratinocytes and fibroblasts leading to the stimulation of elastase. J. Investig. Dermatol. Symp. Proc. 14, 36-43. https://doi.org/10.1038/jidsymp.2009.11
  17. Inomata, S., Matsunaga, Y., Amano, S., Takada, K., Kobayashi,K., Tsunenaga, M., Nishiyama, T., Kohno, Y. and Fukuda, M.(2003). Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse. J. Invest. Dermatol. 120, 128-134. https://doi.org/10.1046/j.1523-1747.2003.12021.x
  18. Kahari, V. M. and Saarialho-Kere, U. (1997). Matrix metalloproteinases in skin. Exp. Dermatol. 6, 199-213. https://doi.org/10.1111/j.1600-0625.1997.tb00164.x
  19. Kim, Y. H., Ryoo, I. J., Choo, S. J., Xu, G. H., Lee, S. K., Seok,S. J., Bae, K. H. and Yoo, I. D. (2009). Clitocybin D, a novel human neutrophil elastase inhibitor from the culture broth of clitocybe aurantiaca. J. Micro. Biotech. 19, 1139-1141.
  20. Kligman, A. M. (1969). Early destructive effect of sunlight on human skin. JAMA 210, 2377-2380. https://doi.org/10.1001/jama.210.13.2377
  21. Labat-Robert, J., Fourtanier, A., Boyer-Lafargue, B. and Robert,L. (2000). Age dependent increase of elastase type protease activity in mouse skin. Effect of UV-irradiation. J. Photochem. Photobiol. B 57, 113-118. https://doi.org/10.1016/S1011-1344(00)00085-3
  22. Lee, S. J., Yun, Y. S., Lee, I. K., Ryoo, I. J., Yun, B. S. and Yoo,I. D. (1999). An antioxidant lignan and other constituents from the root bark of Hibiscus syriacus. Planta Med. 65, 658-660. https://doi.org/10.1055/s-2006-960841
  23. Reunanen, N., Li, S. P., Ahonen, M., Foschi, M., Han, J. andKahari, V. M. (2002). Activation of p38 alpha MAPK enhances collagenase-1 (matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by mRNA stabilization. J. Biol. Chem. 277, 32360-32368. https://doi.org/10.1074/jbc.M204296200
  24. Rittie, L. and Fisher, G. J. (2002). UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1, 705-720. https://doi.org/10.1016/S1568-1637(02)00024-7
  25. Suganuma, K., Nakajima, H., Ohtsuki, M. and Imokawa, G.(2010). Astaxanthin attenuates the UVA-induced up-regulation of matrix-metalloproteinase-1 and skin fibroblast elastase in human dermal fibroblasts. J. Dermatol. Sci. 58, 136-142. https://doi.org/10.1016/j.jdermsci.2010.02.009
  26. Touyz, R. M., He, G., El Mabrouk, M. and Schiffrin, E. L. (2001).p38 Map kinase regulates vascular smooth muscle cell collagen synthesis by angiotensin II in SHR but not in WKY. Hypertension 37, 574-580. https://doi.org/10.1161/01.HYP.37.2.574
  27. Tsuji, N., Moriwaki, S., Suzuki, Y., Takema, Y. and Imokawa, G.(2001). The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem Photobiol 74, 283-290. https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  28. Tsukahara, K., Nakagawa, H., Moriwaki, S., Takema, Y.,Fujimura, T. and Imokawa, G. (2006). Inhibition of ultraviolet-B-induced wrinkle formation by an elastase-inhibiting herbal extract: implication for the mechanism underlying elastaseassociated wrinkles. Int. J. Dermatol. 45, 460-468. https://doi.org/10.1111/j.1365-4632.2006.02557.x
  29. Tsukahara, K., Takema, Y., Moriwaki, S., Tsuji, N., Suzuki, Y.,Fujimura, T. and Imokawa, G. (2001). Selective inhibition of skin fibroblast elastase elicits a concentration-dependent prevention of ultraviolet B-induced wrinkle formation. J. Invest. Dermatol. 117, 671-677. https://doi.org/10.1046/j.0022-202x.2001.01450.x
  30. Tzaphlidou, M. (2004). The role of collagen and elastin in aged skin: an image processing approach. Micron. 35, 173-177. https://doi.org/10.1016/j.micron.2003.11.003
  31. Varani, J., Spearman, D., Perone, P., Fligiel, S. E., Datta, S. C.,Wang, Z. Q., Shao, Y., Kang, S., Fisher, G. J. and Voorhees,J. J. (2001). Inhibition of type I procollagen synthesis by damaged collagen in photoaged skin and by collagenasedegraded collagen in vitro. Am. J. Pathol. 158, 931-942. https://doi.org/10.1016/S0002-9440(10)64040-0
  32. Wiedow, O., Schroder, J. M., Gregory, H., Young, J. A. andChristophers, E. (1990). Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence. J. Biol. Chem. 265, 14791-14795.
  33. Xu, G. H., Kim, Y. H., Chi, S. W., Choo, S. J., Ryoo, I. J., Ahn, J.S. and Yoo, I. D. (2010). Evaluation of human neutrophil elastase inhibitory effect of iridoid glycosides from Hedyotis diffusa. Bioorg. Med. Chem. Lett. 20, 513-515. https://doi.org/10.1016/j.bmcl.2009.11.109
  34. Yoo, I. D., Yun, B. S., Lee, I. K., Ryoo, I. J., Choung, D. H. andHan, K. H. (1998). Three naphthalenes from root bark of Hibiscus syriacus. Phytochemistry 47, 799-802. https://doi.org/10.1016/S0031-9422(97)00674-2

Cited by

  1. Water extract of edible bird’s nest attenuated the oxidative stress-induced matrix metalloproteinase-1 by regulating the mitogen-activated protein kinase and activator protein-1 pathway in human keratinocytes vol.55, pp.3, 2012, https://doi.org/10.1007/s13765-012-2030-8
  2. Protective Effect of 3,4-Dihydroxybenzoic Acid Isolated from Cladophora wrightiana Harvey Against Ultraviolet B Radiation-Induced Cell Damage in Human HaCaT Keratinocytes vol.172, pp.5, 2014, https://doi.org/10.1007/s12010-013-0711-3
  3. Anti-wrinkle effects of Sargassum muticum ethyl acetate fraction on ultraviolet B-irradiated hairless mouse skin and mechanistic evaluation in the human HaCaT keratinocyte cell line vol.14, pp.4, 2016, https://doi.org/10.3892/mmr.2016.5655
  4. Phloroglucinol Attenuates Ultraviolet B Radiation-Induced Matrix Metalloproteinase-1 Production in Human Keratinocytes via Inhibitory Actions against Mitogen-Activated Protein Kinases and Activator Protein-1 vol.88, pp.2, 2012, https://doi.org/10.1111/j.1751-1097.2012.01074.x
  5. Inhibition of Matrix Metalloproteinase-1 Induced by Oxidative Stress in Human Keratinocytes by Mangiferin Isolated from Anemarrhena asphodeloides vol.75, pp.12, 2010, https://doi.org/10.1271/bbb.110465
  6. ROS Scavenging and Anti-Wrinkle Effects of Clitocybin A Isolated from the Mycelium of the Mushroom Clitocybe aurantiaca vol.27, pp.5, 2010, https://doi.org/10.4014/jmb.1702.02050