Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Principal methods to produce marker-free GM plants

무선발표지 형질전환 식물체 제조기술

  • Woo, Hee-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Shin, Kong-Sik (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Ki-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Kweon, Soon-Jong (National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University) ;
  • Suh, Seok-Cheol (National Academy of Agricultural Science, Rural Development Administration (RDA))
  • 우희종 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 신공식 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 이기종 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 권순종 (농촌진흥청 국립농업과학원 생물안전성과) ;
  • 조용구 (충북대학교 식물자원학과) ;
  • 서석철 (농촌진흥청 국립농업과학원 생물안전성과)
  • Received : 2010.03.31
  • Accepted : 2010.04.13
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Selectable marker gene systems are vital for the development of transgenic plants, but the presence of selectable marker genes encoding antibiotic or herbicide resistance in genetically modified plants poses a number of problems. A lot of research results and various techniques have been developed to produce marker-free GM plants. The aim of this review is to describe the principal methods used for eliminating selectable marker genes to generate marker-free GM plants, concentrating on the three significant methods(co-transformation, site-specific recombinase-mediated excision, non-selected transformation) in several marker-free techniques.

Keywords

References

  1. An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol 79:568-570 https://doi.org/10.1104/pp.79.2.568
  2. Ballester A, Cervera M, Pena L (2007) Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination. Plant Cell Rep 26:39-45
  3. Bevan MW, Flavell RB, Chilton MD (1983) A chimeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304:184-187 https://doi.org/10.1038/304184a0
  4. Coppoolse ER, Vroomen MJ, Roelofs D, Smit J, Van Gennip F, Hersmus, BJM, Nijkamp HJJ, Van Kaaren MJJ (2003) Cre recombinase expression can result in phenotypic aberrations in plants. Plant Mol Biol 51:263-279 https://doi.org/10.1023/A:1021174726070
  5. Dale E, Ow D (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci U.S.A. 88:10558-10562 https://doi.org/10.1073/pnas.88.23.10558
  6. Dale PJ, Clarke B, Fontes MG (2002) Potential for the environmental impact of transgenic crops. Nat Biotechnol 20:567-574
  7. Daley M, Knauf VC, Summerfelt KR, Turner JC (1998) Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep 17:489-496 https://doi.org/10.1007/s002990050430
  8. De Neve M, De Buck S, Jacobs A, Van Montagu M, Depicker A (1997) T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs. Plant J 11:15-29 https://doi.org/10.1046/j.1365-313X.1997.11010015.x
  9. Depicker A, Herman L, Jacobs A, Schell J, Van Montagu M (1985) Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium/plant cell interaction. Mol Gen Genet 201:477-484 https://doi.org/10.1007/BF00331342
  10. De Vetten N, Wolters AM, Raemakers K, Van Der Meer I, Ter Stege R, Heeres E, Heeres P, Visser R (2003) A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop. Nat Biotechnol 21:439-442 https://doi.org/10.1038/nbt801
  11. Ebinuma H, Komamine A (2001) MAT (multiauto-transformation) vector system. The oncogenes of Agrobacterium as positive markers for regeneration and selection of marker-free transgenic plants. In Vitro Cell Dev Biol-Plant 37:103-113 https://doi.org/10.1007/s11627-001-0021-2
  12. Ebinuma H, Sugita K, Matsunaga E, Endo S, Yamada K, Komamine A (2001) Systems for the removal of a selection marker and their combination with a positive marker. Plant Cell Rep 20:383-392 https://doi.org/10.1007/s002990100344
  13. Endo S, Sugita K, Sakai M, Tanaka H, Ebinuma H (2002) Single-step transformation for generating marker-free transgenic rice using the ipt-type MAT vector system. Plant J 30:115-122 https://doi.org/10.1046/j.1365-313X.2002.01272.x
  14. Erickson O, Hertzberg M, Nasholm T (2004) A conditional marker gene allowing both positive and negative selection in plants. Nat Biotechnol 22:455-458 https://doi.org/10.1038/nbt946
  15. Gamble J, Gunson A (2002) The New Zealand public's attitudes regarding genetically modified food: May and October 2001. HortResearch Client report No. 2003/35
  16. Gleave AP, Mitra DS, Mudge SR, Morris BAM (1999) Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol Biol 40:223-235 https://doi.org/10.1023/A:1006184221051
  17. Goodwin J, Pastori G, Davey M, Jones H (2004) Transgenic plants: methods and protocols. Methods Mol Biol 286:191-202
  18. Herrera-Estrella L, De Block M, Messens E, Hernalsteens JP, Van Montagu M and Schell J (1983) Chimeric genes as dominant selectable markers in plant cells. EMBO J 2:987-995
  19. James C (2009) Global status of commercialized biotech/GM crops: 2009. ISAAA brief No. 41. ISAAA: Ithaca, NY
  20. Jia HG, Liao MJ, Verbelen JP, Vissenberg K (2007) Direct creation of marker-free tobacco plant from agroinfiltrated leaf discs. Plant Cell Rep 26:1961-1965 https://doi.org/10.1007/s00299-007-0403-y
  21. Joersbo M, Donaldson I, Kreiberg J, Petersen SG, Brunstedt J (1998) Analysis of mannose selection used for transformation of sugar beet. Mol Breeding 4:111-117 https://doi.org/10.1023/A:1009633809610
  22. Jones JDG, Gilbert DE, Grady KL, Jorgensen RA (1987) T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives. Mol Gen Genet 207:478-485 https://doi.org/10.1007/BF00331618
  23. Jorgensen R, Snyder C, Jones JDG (1987) T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives. Mol Gen Genet 207:471-477 https://doi.org/10.1007/BF00331617
  24. Khan RS, Chin DP, Nakamura I, Mii M (2006) Production of marker-free transgenic Nierembergia caerulea using MAT vector system. Plant Cell Rep 25:914-919 https://doi.org/10.1007/s00299-006-0125-6
  25. Kilby NJ, Davies GJ, Snaith MR, Murray JAH (1995) FLP recombinase in transgenic plants: constitutive activity in stably transformed tobacco and generation of marked cell clones in Arabidopsis. Plant J 8:637-652 https://doi.org/10.1046/j.1365-313X.1995.08050637.x
  26. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10:165-174 https://doi.org/10.1046/j.1365-313X.1996.10010165.x
  27. Li B, Xie C, Qiu H (2009) Production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency. Plant Cell Rep 28:373-386 https://doi.org/10.1007/s00299-008-0640-8
  28. Lloyd AM, Davis RW (1994) Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Mol Gen Genet 242:653-657
  29. Luo KM, Duan H, Zhao DG, Zheng XL, Deng W, Chen YQ, Stewart CN, McAvoy R, Jiang XN, Wu YH, He AG, Pei Y, Li Y (2007) ‘GM-gene-deletor’: fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants. Plant Biotechnol J 5:263-374 https://doi.org/10.1111/j.1467-7652.2006.00237.x
  30. Lusk JL, Sullivan P (2002) Consumer acceptance of genetically modified foods. Food Technol 56:32-37
  31. Lyznik LA, Mitchell JC, Hiyarama L, Hodges TK (1993) Activity of yeast FLP recombinase in maize and rice protoplasts. Nucleic Acids Res 21:969-975 https://doi.org/10.1093/nar/21.4.969
  32. Matsunaga E, Sugita K, Ebinuma H (2002) Asexual production of selectable marker-free transgenic woody plants, vegetatively propagated species. Mol Breeding 10:95-106 https://doi.org/10.1023/A:1020308216747
  33. Miki B, McHugh S (2004) Selective marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 107:193-232 https://doi.org/10.1016/j.jbiotec.2003.10.011
  34. Onouchi H, Nishihama R, Kudo M, Machida Y, Machida C (1995) Visualization of site-specific recombination catalyzed by a recombinase from Zygosaccharomyces rouxii in Arabidopsis thaliana. Mol Gen Genet 247:653-660 https://doi.org/10.1007/BF00290396
  35. Onouchi H, Yokoi K, Machida C, Matsuzaki H, Oshima Y, Matsuoka K, Nakamura K, Machida Y (1991) Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells. Nucleic Acids Res 19:6373-6378 https://doi.org/10.1093/nar/19.23.6373
  36. Ow, DW (2002) Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol 48:183-200 https://doi.org/10.1023/A:1013718106742
  37. Privalle LS, Wright M, Reed J, Hansen G, Dawson J, Dunder EM, Chang YF, Powell ML, Meghji M (2000) Phosphomannose isomerase-novel system for plant selection: mode of action and safety assessment. In: Fairburn C, Scoles G, McHughen A (Eds.) Proceedings of the 6th International Symposium on the Biosafety of Genetically Modified Organisms. pp. 171-178
  38. Reed J, Privall L, Powell ML, Meghji M, Dawson J, Dunder E, Suttie J, Wenck A, Launis K, Kramer C, Chang YF, Hansen G, Wright M (2001) Phosphomannose isomerase: an efficient selectable marker for plant transformation. In Vitro Cell Dev Biol-Plant 37:127-132
  39. Russell SH, Hoopes JL, Odell JT (1992) Directed excision of a transgene from the plant genome. Mol Gen Genet 234:49-59
  40. Schaart JG (2004) Towards consumer-friendly cisgenic strawberries which are less susceptible to Botrytis cineres. PhD thesis, Wageningen University, Wageningen, Netherlands
  41. Sonti RV, Tisser AF, Wong D, Viret JF, Signer ER (1995) Activity of the yeast FLP recombinase in Arabidopsis. Plant Mol Biol 28:1127-1132 https://doi.org/10.1007/BF00032673
  42. Sreekala C, Wu L, Gu Wang D, Tian D (2005) Excision of a selectable marker in transgenic rice (Oryza sativa L.) using a chemically regulated CRE/loxP system. Plant Cell Rep 24:86-94 https://doi.org/10.1007/s00299-004-0909-5
  43. Sugita K, Kasahara T, Matsunaga E, Ebinuma H (2000) A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J 22:461-469 https://doi.org/10.1046/j.1365-313X.2000.00745.x
  44. Sugita K, Matsunaga E, Ebinuma H (1999) Effective selection system for generating marker-free transgenic plants independent of sexual crossing. Plant Cell Rep 18:941-947 https://doi.org/10.1007/s002990050688
  45. Woo HJ, Cho HS, Lim SH, Shin KS, Lee SM, Lee KJ, Kim DH, Cho YG (2009) Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system. Transgenic Res 18:455-465 https://doi.org/10.1007/s11248-008-9236-x
  46. Wu L, Nandi S, Chen L, Rodriguez RL, Huang N (2002) Expression and inheritance of nine transgenes in rice. Transgenic Res 11:533-541 https://doi.org/10.1023/A:1020331608590
  47. Zelasco S, Ressegotti V, Confalonieri M, Carbonera D, Calligari P, Bonadei M, Bisoffi S, Yamada K, Balestrazzi A (2007) Evaluation of MAT-vector system in white poplar (Populus alba L.) and production of ipt marker-free transgenic plants by ‘single-step transformation’. Plant Cell Tissue Organ Cult 91:61-72 https://doi.org/10.1007/s11240-007-9278-4
  48. Zhang Y, Li H, Ouyang B, Lu Y and Ye Z (2006) Chemical-induced autoexcision of selectable markers in elite tomato plants transformed with a gene conferring resistance to lepidopteran insects. Biotechnol Lett 28:1247-1253 https://doi.org/10.1007/s10529-006-9081-z
  49. Zuo J, Niu QW, Ikeda Y, Chua NH (2002) Marker-free transformation: increasing frequency by the use of regeneration-promoting genes. Cur Opin in Biotech 13:173-180 https://doi.org/10.1016/S0958-1669(02)00301-4
  50. Zuo J, Niu QW, Moller SG, Chua NH (2001) Chemical-regulated, site-specific DNA excision in transgenic plants. Nat Biotechnol 19:157-161 https://doi.org/10.1038/84428