Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds

  • Oh, Doo-Ri (Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University) ;
  • Cheong, Il-Cheong (Hankyong National University) ;
  • Lee, Hee-Gu (Medical Genomic Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Eo, Seong-Kug (Department of Microbiology, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Kang, Seong-Ho (Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University)
  • Received : 2010.01.12
  • Accepted : 2010.05.05
  • Published : 2010.07.20
Download PDF
⟨ Previous Next ⟩

Abstract

A microchip electrophoresis (ME) method was developed using a programmed field strength gradients (PFSG) for the single nucleotide polymorphism (SNP) based fast identification of cattle breeds. Four different Korean cattle (Hanwoo) and Holstein SNP markers amplified by allele-specific polymerase chain reaction were separated in a glass microchip filled with 0.5% poly(ethyleneoxide) ($M_r$ = 8 000 000) by PFSG as follows: 750 V/cm for 0 - 14 s, 166.7 V/cm for 14 - 31 s, 83.3 V/cm for 31 - 46 s, and 750 V/cm for 46 - 100 s. The cattle breeds were clearly distinguished within 45 s. The ME-PFSG method was 7 times and 5 times faster than the constant electric field ME method and the capillary electrophoresis- PFSG method, respectively, with a high resolving power ($R_s$ = 5.05 - 9.98). The proposed methodology could be a powerful tool for the fast and simultaneous determination of SNP markers for various cattle breeds with high accuracy.

Keywords

References

  1. Gielbert, A.; Davis, L. A.; Sayers, A. R.; Hope, J.; Gill, A. C.; Sauer,M. J. J. Mass. Spectrom. 2000, 33, 384.
  2. Do, K. T.; Shin, H. Y.; Lee, J. H.; Kim, N. S.; Park, E. W.; Yoon, D.H.; Kim, K. S. J. Anim. Sci. & Technol. 2007, 49, 711. https://doi.org/10.5187/JAST.2007.49.6.711
  3. Kim, D.-K.; Kang, S. H. J. Chromatogr. A 2005, 1064, 121. https://doi.org/10.1016/j.chroma.2004.12.045
  4. Lee, M.; Yoon, D.; Jeon, J.-T.; Eo, S. K.; Kang, S. H. Bull. Korean Chem. Soc. 2009, 30, 2655. https://doi.org/10.5012/bkcs.2009.30.11.2655
  5. Jeon, S.; Eo, S. K.; Kim, Y.; Yoo, D. J.; Kang, S. H. Talanta 2007,73, 415. https://doi.org/10.1016/j.talanta.2007.04.002
  6. Heller, C. Electrophoresis 2000, 21, 593. https://doi.org/10.1002/(SICI)1522-2683(20000201)21:3<593::AID-ELPS593>3.0.CO;2-U
  7. Luckey, J. A.; Smith, L. M. Anal. Chem. 1993, 65, 2841. https://doi.org/10.1021/ac00068a022
  8. Guttman, A.; Cooke, N. J. Chromatogr. 1991, 559, 285. https://doi.org/10.1016/0021-9673(91)80078-U
  9. Baker, D. R. Capillary Electrophoresis; John Wiley & Sons: New York, 1995; pp 31-52.
  10. Guttman, A.; Wanders, B.; Cooke, N. Anal. Chem. 1992, 64, 2348. https://doi.org/10.1021/ac00044a009
  11. Guttman, A.; Cooke, N. Anal. Chem. 1991, 63, 2038. https://doi.org/10.1021/ac00018a027
  12. Karger, B. L.; Cohen, A. S.; Guttman, A. J. Chromotogr. 1989, 492,585. https://doi.org/10.1016/S0378-4347(00)84480-1
  13. Demana, T.; Lanan, M.; Morris, M. D. Anal. Chem. 1991, 63, 2795. https://doi.org/10.1021/ac00023a023
  14. Yoshida, C.; Endo, Y.; Baba, Y. Eur. J. Pharm. Sci. 2001, 13, 99. https://doi.org/10.1016/S0928-0987(00)00213-X
  15. Inoue, H.; Tsuhako, M.; Baba, Y. J. Chromatogr. A 1998, 802,179. https://doi.org/10.1016/S0021-9673(97)01098-4
  16. Go, H.; Lee, M.; Oh, D.; Kim, K.-S.; Cho, K.; Yoo, D. J.; Kang,S. H. Bull. Korean Chem. Soc. 2009, 30, 2141. https://doi.org/10.5012/bkcs.2009.30.9.2141
  17. Tang, G.; Yan, D.; Yang, C.; Gong, H.; Chai, J. C.; Lam, Y. C. Electrophoresis2006, 27, 628. https://doi.org/10.1002/elps.200500681
  18. Evenhuis, C. J.; Haddad, P. R. Electrophoresis 2009, 30, 897. https://doi.org/10.1002/elps.200800643
  19. Negrini, R.; Nicoloso, L.; Crepaldi, P.; Milanesi, E.; Colli, L.;Chegdani, F.; Pariset, L. et al. Anim. Genet. 2009, 40, 18. https://doi.org/10.1111/j.1365-2052.2008.01800.x

Cited by

  1. Theoretical investigation on the performance of DNA electrophoresis under programmed step electric field strength: Two-step condition vol.38, pp.20, 2015, https://doi.org/10.1002/jssc.201500640
  2. Voltage-programming-based capillary gel electrophoresis for the fast detection of angiotensin-converting enzyme insertion/deletion polymorphism with high sensitivity vol.39, pp.16, 2016, https://doi.org/10.1002/jssc.201600439
  3. Food analysis on microchip electrophoresis: An updated review vol.33, pp.15, 2010, https://doi.org/10.1002/elps.201200049