Antioxidant Effects of the Methanol Extracts Obtained from Aerial Part and Rhizomes of Ferns Native to Korea

자생 양치식물 지상부와 근경 메탄올 추출물의 항산화 효과

  • Shin, So-Lim (Dept. of Horticultural Science, Chungbuk Nat'l Univ.) ;
  • Lee, Cheol-Hee (Dept. of Horticultural Science, Chungbuk Nat'l Univ.)
  • 신소림 (충북대학교 응용생명환경학부 원예과학과) ;
  • 이철희 (충북대학교 응용생명환경학부 원예과학과)
  • Received : 2009.10.12
  • Accepted : 2010.01.22
  • Published : 2010.02.28

Abstract

Phenolic compound contents and scavenging activity on DPPH and ABTS radicals in twelve fern species were studied by analysing ultrasonification extraction of dried aerial parts and rhizomes using methanol solvent. Total polyphenol content ranged from 2.97 to 140.1 mg per 1 g dried sample and 0.80 to $49.58\;mg{\cdot}g^{-1}$ fresh sample. Highest polyphenol content was obtained with aerial part of Davallia mariesii and Polystichum lepidocaulon. Total flavonoid content of dried sample was $2.56{\sim}34.91\;mg{\cdot}g^{-1}$ and fresh sample $0.71{\sim}11.49\;mg{\cdot}g^{-1}$. Higher flavonoids were obtained with dried aerial part of Dryopteris crassirhizoma, but with fresh aerial part of P. lepidocaulon. In general, total polyphenol content was higher in rhizomes, except in case of total flavonoid. Scavenging effects on DPPH and ABTS radicals was higher with rhizome extracts compared to aerial parts. The rhizome of Polystichum polyblepharum showed highest effects on both radicals. However, the amount of fresh sample for $RC_{50}$ with consideration of water content and extraction yield. P. lepidocaulon aerial part was more advantageous than P. polyblepharum rhizome. All the fern extracts except for the Coniogramme japonica extract demonstrated superior scavenging effects on ABTS radicals, being similar activity of ascorbic acid and BHT.

메탄올을 용매로 12종 양치식물의 지상부와 근경의 건조시료를 초음파추출하여 페놀성 물질 함량 및 DPPH와 ABTS radical 소거능을 측정하였다. 총 폴리페놀의 함량은 건조시료 1 g에 $2.793{\sim}140.014\;mg{\cdot}g^{-1}$, 생시료 1 g에 $0.779{\sim}49.579\;mg{\cdot}g^{-1}$으로 다양하게 나타났으며, 넉줄고 사리와 더부살이고사리의 지상부에서 총 폴리페놀 함량이 높았다. 총 플라보노이드 함량은 건조시료 1 g에 $2.559{\sim}34.909\;mg{\cdot}g^{-1}$, 생시료 1g에 $0.714{\sim}11.487\;mg{\cdot}g^{-1}$으로 나타났다. 건조시료 중에는 관중 지상부, 생시료 중에는 더 부살이고사리의 지상부에서 총 플라보노이드 함량이 가장 높았다. 총 폴리페놀은 지상부보다 근경에 많이 함유된 경향을 보였으나, 총 플라보노이드는 지상부에 많았다. DPPH와 ABTS radical 소거능은 지상부보다 근경 추출물에서 높게 나타났다. 총 폴리페놀은 지상부보다 근경에 많이 함유된 경향을 보였으나, 총 플라보노이드는 지상부에서 함량이 많았다. DPPH와 ABTS radical 소거능은 지상부보다 근경 추출물에서 높게 나타났다. DPPH radical 소거능과 ABTS radical 소거능 모두 나도히초미 근경 추출물에서 가장 우수하였다. 그러나 시료의 수분함량과 추출수율을 고려하여 $RC_{50}$을 나타내는데 필요한 생시료의 양을 계산한 결과, 나도히초미 근경보다는 더부살이고사리 지상부가 DPPH와 ABTS radical 소거용 식물소재로 적합하였다. 양치식물의 추출물은 특히 ABTS radical 소거능이 매우 우수하였으며, 가지고비고사리 추출물을 제외한 대부분의 시료가 ascorbic acid 또는 BHT와 유사한 소거활성을 보였다.

Keywords

References

  1. Ahn, D.K. 2003. Illustrated book of Korean medical herbs. Gyohaksa, Seoul.(in Korean)
  2. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1199-1204.
  3. Cha, J.Y., H.Y. Ahn, K.E. Eom, B.K. Park, B.S. Jun, and Y.S. Cho. 2009. Antioxidative activity of Aralia elata shoot and leaf extracts. J. Life Sci. 19:652-658.(in Korean) https://doi.org/10.5352/JLS.2009.19.5.652
  4. Jeong, J.A., S.H. Kwon, and C.H. Lee. 2007. Screening for antioxidative activities of extracts from aerial and underground parts of some edible and medicinal ferns. Kor. J. Plant Res. 20:185-192.(in Korean)
  5. Jung, S.J., J.H. Lee, H.N. Song, N.S. Seong, S.E. Lee, and N.I. Baek. 2004. Screening for antioxidant activity of plant medicinal extracts. J. Kor. Soc. Appl. Biol. Chem. 47:135-140.(in Korean)
  6. Kawaguchi, K., T. Mizuno, K. Aida, and K. Uchino. 1997. Hesperidin as an inhibitor of lipases from porcine pancreas and pseudomonas. Biosci. Biotechnol. Biochem. 61:102-104. https://doi.org/10.1271/bbb.61.102
  7. Kim, E.Y., I.H. Baik, J.H. Kim, S.R. Kim, and M.R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Technol. 36:333-338.(in Korean)
  8. Korean Fern Society(KFS). 2005. Illustrated fern native to Korea. Geobook, Seoul.(in Korean)
  9. Lee, S.O., H.J. Lee, M.H. Yu, H.G. Im, and I.S. Lee. 2005a. Total polyphenol contents and antioxidant activities of metanol extracts from vegetables produced in Ullung Island. Kor. J. Food Sci. Technol. 37:233-240.(in Korean)
  10. Lee, Y.A., H.Y. Kim, and E.J. Cho. 2005b. Comparaison of methanol extracts from vegetables on antioxidative effect under In Vitro and cell system. J. Kor. Soc. Food Sci. Nutr. 34:1151-1156.(in Korean) https://doi.org/10.3746/jkfn.2005.34.8.1151
  11. Li, H., Y.M. Choi, J.S. Lee, J.S. Park, K.S. Yeon, and C.D. Han. 2007. Druing and antioxidant characteristics of the shiitake (Lentinus edodes) mushroom in a conveyer-type far-ingrared dryer. J. Kor. Soc. Food Sci. Nutr. 36:250-254.(in Korean) https://doi.org/10.3746/jkfn.2007.36.2.250
  12. Lim, R.J. 1999. Medical herbal of Josun. Korean Culture Publishing Co., Ltd., Seoul.(in Korean)
  13. Nam, K.H. and Y.M. Lee. 2005. Edible ferns of Korea. J. Kor. Ferns Soc. 9:23-30.(in Korean)
  14. NFRI. 1990. Manuals of quality characteristic analysis for food quality evaluation(2). National Food Research Institute, Skuba.
  15. Oh, S.J., S.S. Hong, Y.H. Kim, and S.C. Koh. 2008. Screening of biological activities in fern plants native to Jeju Island. Kor. J. Plant Res. 21:12-18.(in Korean)
  16. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  17. Shi, J., J. Gong, J. Liu, X. Wu, and Y. Zhang. 2009. Antioxidant capacity of extract from edible flowers of Prunus mume in China and its active components. LWT-Food Sci. Thechnol. 42:477-482. https://doi.org/10.1016/j.lwt.2008.09.008
  18. Shin, J.H., S.J. Lee, J.K. Seo, E.W. Cheon, and N.J. Sung. 2008. Antioxidant activity of hot-water extract from Yuza(Citrus junos Sieb ex Tanaka) Peel. J. Life Sci. 18:1745-1751.(in Korean) https://doi.org/10.5352/JLS.2008.18.12.1745
  19. Velioglu, Y.S., G. Mazza, L. Cao, and B.D. Oomah. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food Chem. 46:4113-4117. https://doi.org/10.1021/jf9801973
  20. Woo, J.H., H.S. Jeong, J.S. Yu, Y.D. Chang, and C.H. Lee. 2008. Antiosidant effect of extracts obtained from four Aster species native to Korea. Kor. J. Plant Res. 21:52-59.(in Korean)