The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Effects of Bupleuri radix Extract on Axon Regrowth in the Injured Sciatic Nerve of Rats

흰쥐의 좌골신경축삭 압좌 손상 후 시호(柴胡) 추출물에 의한 재생반응성 개선효과

  • Kang, Jun-Hyuk (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Daejeon University) ;
  • Oh, Min-Seok (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Daejeon University)
  • 강준혁 (대전대학교 한의과대학 한방재활의학과교실) ;
  • 오민석 (대전대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.08.06
  • Accepted : 2009.09.09
  • Published : 2010.01.30
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Abstract

Objectives: The present study was performed to evaluate the potential effects of Bupleuri radix (SH) on regenerative activities in the peripheral sciatic nerve after crushing injury in rats. Methods: Axonal regeneration after crush injury in rats was analyzed by immunofluorescence staining using anti-NF-200 antibody and retrograde tracing of DiI-axons. Changes in protein levels in the sciatic nerve axons and DRG tissue were analyzed by Western blot analysis and immunofluorescence staining. Effects of SH extract treatment on neurite outgrowth was examined by immunofluorescence staining for cultured DRG neurons. Results: Major findings on the effects of SH extract treatment on axonal regeneration are summarized as follows. 1. SH-mediated enhancement in axonal regeneration was identified by immuno- fluorescence straining of NF-200 protein and retrograde tracing of DiI-labeled axons. 2. Axonal GAP-43 protein levels were upregulated by SH not only in the injured axons but also in the DRG sensory neurons corresponding to sciatic sensory axons. 3. Phospho-Erk1/2 protein levels were increased in both injured axonal area and DRG sensory neurons by SH. Phospho-Erk1/2 was also found in non-neuronal cells in the injured axons. 4. SH elevated levels of Cdc2 protein produced in Schwann cells in the distal portions of injured sciatic nerves. 5. The neurite outgrowth of DRG sensory neurons in culture was augmented by SH, and these changes were positively associated with GAP-43 production levels in the DRG neurons. Conclusions: These data suggest that SH extract improves the regenerative responses of injured peripheral neurons, and thus may be useful for understanding molecular basis for the development of therapeutic strategies.

Keywords

References

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