The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

Gene expression microarray analysis of Paeoniae radix on IL-$1{\beta}$-stimulated primary human gingival fibroblast

Microarray를 이용한 작약(芍藥)의 인간치은섬유모세포 유전자 발현 조절 연구

  • Kim, Kyung-Ho (Dept. of Applied Eastern Medicine, Grad. School, Kyung Hee University) ;
  • Choi, Yeong-Gon (Dept. of Applied Eastern Medicine, Grad. School, Kyung Hee University) ;
  • Hong, Yeon-Mi (WHO Collaborating Ctr. for Traditional Medicine, East-West Med. Res. Institute) ;
  • Yeo, Su-Jung (WHO Collaborating Ctr. for Traditional Medicine, East-West Med. Res. Institute) ;
  • Choi, Ji-Hoon (WHO Collaborating Ctr. for Traditional Medicine, East-West Med. Res. Institute) ;
  • Kim, Young-Hong (Institute for Oral Health, IL-WOL Oriental Medicine Clinic) ;
  • Lee, Je-Hyun (Dept. of Herbology, Col. of Eastern Medicine, Dongguk University) ;
  • Lim, Sa-Bi-Na (Dept. of Applied Eastern Medicine, Grad. School, Kyung Hee University)
  • 김경호 (경희대학교 대학원 한방응용의학과) ;
  • 최영곤 (경희대학교 대학원 한방응용의학과) ;
  • 홍연미 (경희대학교 동서의학연구소) ;
  • 여수정 (경희대학교 동서의학연구소) ;
  • 최지훈 (경희대학교 동서의학연구소) ;
  • 김영홍 (일월한의원 구강건강연구소) ;
  • 이제현 (동국대학교 한의과대학 본초학교실) ;
  • 임사비나 (경희대학교 대학원 한방응용의학과)
  • Received : 2010.01.11
  • Accepted : 2010.02.25
  • Published : 2010.03.30
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Abstract

Background & Objective: The aim of this study was to investigate the effect of P. radix on the inflammatory related gene expression in IL-$1{\beta}$-stimulated primary human gingival fibroblast using Whole Transcript Sense Target (WT-ST). Method: Human gingival fibroblast was incubated with P. radix [100 or $200\;{\mu}g/ml$], and IL-$1{\beta}$ [$1ng/m{\ell}$] added an hour later. After 24h, total RNA was extracted using RNeasy Mini Kit and the whole gene expression patterns were performed using WT-ST Labeling $Assay^{(R)}$. Result: In the DEG results, 782 genes were up-regulated in the IL-$1{\beta}$-treated group as compared to control and among those, 43 genes were associated with inflammation. 981 genes were down-regulated after treatment with IL-$1{\beta}$ and of those 7 genes were associated with inflammation. 1439 genes were up-regulated after treatment with P. radix plus IL-$1{\beta}$-treated when compared to IL-$1{\beta}$-treated alone group and 1225 genes were down-regulated in the same condition. Among the down-regulated genes, 5 were associated with inflammation- and inhibitor genes such as GDF15 and LIF. In the analysis of the P. radix plus IL-$1{\beta}$-treated group, the most significant pathways were the cytokine-cytokine receptor interaction, toll-like receptor signaling, JAK-STAT signaling and tyrosine metabolism. The gene expression patterns in the P. radix $200{\mu}g/m{\ell}$ plus IL-$1{\beta}$-treated group appear to be more involved in the metabolism-related pathways than in the $100{\mu}g/m{\ell}$ plus IL-$1{\beta}$-treated group. Conclusion & Discussion: By microarray analysis of gene expression data, we are able to identify gene expression patterns associated with not only anti-inflammation effect but also transcription function of P. radix.

Keywords

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