The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Skin Application of Gelidium amansii Extract on Deep Second-degree Burns in Mice

석화채(石花菜) 추출물 도포가 마우스의 심재성 2도 화상에 미치는 영향

  • Kim, Dong-Hyun (Dept. of Oriental Medical Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongguk University) ;
  • Hong, Seung-Ug (Dept. of Oriental Medical Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongguk University)
  • 김동현 (동국대학교 한의과대학 안이비인후피부과학교실) ;
  • 홍승욱 (동국대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2009.12.02
  • Accepted : 2010.02.09
  • Published : 2010.03.30
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Abstract

Objective: The purpose of this study was to investigate the effects of skin application with Gelidium amansii extract on skin with deep second degree burns in mice. Methods: BALB/c mice were divided into four groups: normal (NOR) group; burn-elicited mice (CON) group, Silmazine-treated mice after burn elicitation (ST) group, and Gelidium amansii-extract treated mice after burn elicitation (GT) group. To examine the skin recovery effect after burn, changes of burn area, angiogenesis and histologic structure were analyzed. To measure effect of edema regulation, matrix metalloproteinase-9 (MMP-9) was analyzed. To estimate the skin regenerative & stable effect, 5-bromo-2'-deoxyuridine (BrdU) and substance P were analyzed. Results: 2 weeks later, 1. The size of burn area decreased in the GT and ST groups more than the CON group. 2. Alleviation of angiogenesis appeared in the GT and ST groups more than in the CON group. 3. Blood clot, epithelial cell hyperplasia, and inflammatory cell infiltration declined in the GT and ST groups more than in the CON group. 4. MMP-9, BrdU, and substance P positive reaction decreased in the GT and ST groups more than in the CON group 5. In the comparative study, the GT group was superior to the ST group. Conclusion: The skin application of Gelidium amansii extract could lessen skin damage by the medium of regulation MMP-9 activation. This skin stabilization was induced in mice with deep second degree burns.

Keywords

References

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