Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Determination of Hyperin in the Fruits of Acanthopanax Species by High Performance Liquid Chromatography

  • Lee, Jeong-Min (Department of Applied Plant Science, Chung-Ang University) ;
  • Kim, Hye-Min (Department of Applied Plant Science, Chung-Ang University) ;
  • Lee, Su-Llim (Department of Applied Plant Science, Chung-Ang University) ;
  • Han, Saem (Department of Applied Plant Science, Chung-Ang University) ;
  • Cho, Seon-Haeng (Gongju National University of Education) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, Chung-Ang University)
  • Received : 2010.01.01
  • Accepted : 2010.03.17
  • Published : 2010.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The content of hyperin in Acanthopanax species was determined by high performance liquid chromatography (HPLC). Hyperin was quantified by a reverse-phase column with elution program [initially gradient solvent (acetonitrile : water = 85 : 15 to 80 : 20 for 20 min), then isocratic solvent (acetonitrile : water = 80 : 20 for 20 min), and finally gradient solvent (acetonitrile : water = 80 : 20 to 65 : 35 for 20 min)]. UV detection was conducted at 210 nm. The content of hyperin in the fruits of Acanthopanax was measured in the species A. chiisanensis (2.04 mg/g), A. sessiliflorus (1.13 mg/g), A. divaricatus (0.98 mg/g), A. koreanum (0.75 mg/g) and A. senticosus (0.05 mg/g). The content of hyperin in A. chiisanensis was higher than that of other Acanthopanax species.

Keywords

References

  1. Chen, M., Song, F., Guo, M., Liu, Z., and Liu, S., Analysis of flavonoid constituents from leaves of Acanthopanax senticosus Harms by electrospray tandem mass spectrometry. Rapid Commun Mass Spec., 16, 264-271 (2002). https://doi.org/10.1002/rcm.574
  2. Chinnici, F., Gaiani, A., Natali, N., Riponi, C., and Galassi, S., Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolitinic column. J. Agric. Food. Chem., 52, 3-7 (2004). https://doi.org/10.1021/jf030459n
  3. Davydov, M. and Krikorian, A.D., Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as adaptogen: a closer look. J. Ethnopharmacol., 72, 345-393 (2000). https://doi.org/10.1016/S0378-8741(00)00181-1
  4. Fang, L., Li, W., Li, H., Zhang, Q., and Koike, K., Pancreatic lipase inhibiting triterpenoid saponins from fruits of Acanthopanax senticosus. Chem. Pharm. Bull., 55, 1087-1089 (2007). https://doi.org/10.1248/cpb.55.1087
  5. Han, J.T., Oh, S.J., Kim, H.Y., Park, Y.D., and Baek, N.I., Hyperin, antioxidant compounds isolated from the branch of Uncaria rhynchophylla Miq. J. Korean Soc. Agric. Chem. Biotechnol., 43, 78-80 (2000).
  6. Kang, J.S., Linh, P.T., Cai, X.F., Lee, J.J., and Kim, Y.H., Determination of chiisanoside in Acanthopanax species by high performance liquid chromatography. Nat. Prod. Sci., 9, 45-48 (2003).
  7. Kim, M.K., Kim, Y.S., Heo, S.I., Shim, T.H., Sa, J.H., and Wang, M.H., Studies for component analysis and antioxidant effect, antimicrobial activity in Acanthopanax senticosus HARMS. Kor. J. Pharmacogn., 37, 151-156 (2006).
  8. Lee, J.M., Kim, H.M., and Lee, S., Quantitative analysis of chiisanoside in Acanthopanax species by HPLC. Nat. Prod. Sci., 13, 148-151 (2007).
  9. Lee, S., Kim, B.K., Cho, S.H., and Shin, K.H., Phytochemical constituents from the fruits of Acanthopanax sessiliflorus. Arch. Pharm. Res., 25, 280-284 (2002). https://doi.org/10.1007/BF02976626
  10. Lee, S., Chung, H.S., Shin, K.H., and Kim, B.K., Determination of hyperin in Acanthopanax senticosus and A. sessiliflorus by HPLC. Yakhak Hoeji, 4, 231-235 (2004a).
  11. Lee, S., Jung, S.H., Lee, Y.S., and Shin, K.H., Hyperin, an aldose redictase inhibitor from Acanthopanx senticosus leaves. Nat. Prod. Sci., 9, 4-6 (2003).
  12. Lee, S., Jung, S.H., Lee, Y.S., Yamada, M., Kim, B K., Ohuchi, K., and Shin, K.H., Anti-inflammatory activity of hyperin from Acanthopanax chiisanensis roots. Arch. Pharm. Res., 27, 628-632 (2004b). https://doi.org/10.1007/BF02980162
  13. Lommen, A., Godejohann, M., Venema, D.P., Hollman, P.C., and Spraul, M., Application of directly coupled HPLC-NMR-MS to the identification and confirmation of quercetin glycosides and phloretin glycosides in apple peel. Anal. Chem., 72, 1793-1797 (2000). https://doi.org/10.1021/ac9912303
  14. Perry, L.M. and Metzger, J., Medicinal plants of East and Southeast Asia. MIT press, Cambridge, Massachusetts and London, p. 41 (1980).
  15. Sha, M., Cao, A., and Yang, S., Determination of hyperin in Epimedium koreanum Nakai by HPLC. China J. Chin. Mat. Med., 20, 357-358 (1995).
  16. Sha, M., Cao, A., Wang, B., Liu, C., Geng, J., and Liu, W., Determination of hyperin in Sanguisorba officinalis L. by high performance liquid chromatography. China J. Chromatogr., 16, 226-228 (1998).
  17. Shin, K.H. and Lee, S., The chemistry of secondary products from Acanthopanax species and their pharmacological activities (Review). Nat. Prod. Sci., 8, 111-126 (2002).
  18. Wu, Y., Zhou, S.D., and Li, P., Determination of flavonoids in Hypericum perforatum by HPLC analysis. Acta. Pharmaceut. Sin., 37, 280-282 (2002).
  19. Yook, C.S., Coloured Medicinal Plants of Korea. Academy Publishing Co., Seoul, Korea, p. 372 (1990).