Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Determination of Paeoniflorin, Trans-cinnamic Acid, Schisandrin and Glycyrrhizin in So-Cheong-Ryong-Tang by HPLC-DAD and HPLC-ESI-MS

  • Lee, Mi-Kyeong (Institute for Life Science, Elcom Science Co. Ltd.) ;
  • Lee, Ki-Yong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Park, Jung-Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University) ;
  • Kim, Seung-Hyun (Institute for Life Science, Elcom Science Co. Ltd.) ;
  • Choi, Ok-Gyung (Institute for Life Science, Elcom Science Co. Ltd.) ;
  • Park, Jin-Ho (Institute for Life Science, Elcom Science Co. Ltd.) ;
  • Cho, Jung-Hee (Herbal Medicine Evaluation Department, Korea Drug & Food Administration) ;
  • Kim, Do-Hoon (Herbal Medicine Evaluation Department, Korea Drug & Food Administration) ;
  • Baek, Ju-Hyun (Herbal Medicine Evaluation Department, Korea Drug & Food Administration) ;
  • Oh, Mi-Hyune (Herbal Medicine Evaluation Department, Korea Drug & Food Administration) ;
  • Kim, Hyo-Jin (College of Pharmacy, Dongduk Women's University) ;
  • Sung, Sang-Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
  • Received : 2009.08.20
  • Accepted : 2009.12.29
  • Published : 2010.03.31
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Abstract

High performance liquid chromatographic method with diode-array detection (HPLC-DAD) has been performed for the simultaneous determination of four marker constituents, paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in traditional herbal medicinal preparation, So-Cheong-Ryong-Tang (SCRT). The presence of paeoniflorin, trans-cinnamic acid, schisandrin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard, UV spectrum and ESI mass spectrum. All four compounds showed good linearity ($r^2$ > 0.998) in a relatively wide concentration ranges. The RSD for intra-day and inter-day precision was less than 3% and the limits of detection (LOD) were less than 30 ng. The mean recovery of each compound was 94.1-113.0% with RSD values less than 3.0%. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial SCRT products.

Keywords

References

  1. Amagaya, S., Iizuka, A., Makino, B., Kubo, M., Komatsu, Y., Cheng, F.C., Ruo, T.I., Itoh, T., and Terasawa, K., General pharmacological properties of Sho-seiryu-to (TJ-19) extracts. Phytomedicine 8, 338-347 (2001). https://doi.org/10.1078/0944-7113-00061
  2. Antonnen, M.J., Hoppula, K.I., Nestby, R., Verheul, M.J., and Karjalainen, R.O., Influence of fertilization, mulch color, early forcing, fruit order, planting date, shading, growing environment, and genotype on the contents of selected phenolics in strawberry (Fragaria x ananassa Duch.) fruits. J. Agric. Food Chem. 54, 2614-2620 (2006). https://doi.org/10.1021/jf052947w
  3. Dong, H., Liu, Z., Song, F., Yu, Z., Li, H., and Liu, S., Structural analysis of monoterpene glycosides extracted from Paeonia lactiflora Pall. using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun. Mass Spectrom. 21, 3193-3199 (2007).
  4. Halstead, C.W., Lee, S., Khoo, C.S., Hennell, J.R., and Bensoussan, A., Validation of a method for the simultaneous determination of four schisandra lignans in the raw herb and commercial dried aqueous extracts of Schisandra chinensis (Wu Wei Zi) by RP-LC with DAD. J. Pharm. Biomed. Anal. 45, 30-37 (2007). https://doi.org/10.1016/j.jpba.2007.05.016
  5. He, Z.D., Qiao, C.F., Han, Q.B., Cheng, C.L., Xu, H.X., Jiang, R.E., and Shaw, P.C., Authentication and quantitative analysis on the chemical profile of cassia bark (cortex cinnamomi) by high-pressure liquid chromatography. J. Agric. Food Chem. 53, 2424-2428 (2005). https://doi.org/10.1021/jf048116s
  6. Jong, T.T., Lee, M.R., Chiang, Y.C., and Chiang, S.T., Using LC/MS/MS to determine matrine, oxymatrine, ferulic acid, mangiferin, and glycyrrhizin in the Chinese medicinal preparations Shiau-feng-saan and Dang-guei-nian-tong-tang. J. Pharm. Biomed. Anal. 40, 472-477 (2006). https://doi.org/10.1016/j.jpba.2005.07.036
  7. Jung, J.H., Park, S.I., Park, S.S., and Park, J.H., Studies on physical and chemical quality evaluation of crude drugs preparation. Analysis of So Cheong Ryong Tang. Kor. J. Pharmacogn. 29, 28-34 (1998).
  8. Kao, S.T., Lin, C.S., Hsieh, C.C., Hsieh, W.T., and Lin, J.G., Effects of xiao-qing-long-tang (XQLT) on bronchoconstriction and airway eosinophil infiltration in ovalbumin-sensitized guinea pigs: in vivo and in vitro studies. Allergy 56, 1164-1171 (2001).
  9. Ko, E., Rho, S., Cho, C., Choi, H., Ko, S., Lee, Y., Hong, M.C., Shin, M.K., Jung, S.G., and Bae, H., So-Cheong-Ryong-Tang, tradititional Korean medicine, suppresses Th2 lineage development. Biol. Pharm. Bull. 27,739-743 (2004). https://doi.org/10.1248/bpb.27.739
  10. Li, C.Y., Chiu, C.H., Huang, H.S., Lin, C.H., and Wu, T.S., Highperformance liquid chromatographic method for simultaneous quantification of eight major biologically active ingredients in 'Da-Chai-Hu-Tang' preparation. Biomed. Chromatogr. 20, 305-308 (2006). https://doi.org/10.1002/bmc.572
  11. Okamura, N., Miki, H., Orii, H., Masaoka, Y., Yamashita, S., Kobayashi, H., and Yagi, A., Simultaneous high-performance liquid chromatographic determination of puerarin, daidzin, paeoniflorin, liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizin in Kampo medicines. J. Pharm. Biomed. Anal. 19, 603-612 (1999a). https://doi.org/10.1016/S0731-7085(98)00262-3
  12. Okamura, N., Miki, H., Harada, T., Yamashita, S., Masaoka, Y., Nakamoto, Y., Tsuguma, M., Yoshitomi, H., and Yagi, A., Simultaneous determination of ephedrine, pseudoephedrine, norephedrine and methylephedrine in Kampo medicines by high-performance liquid chromatography. J. Pharm. Biomed. Anal. 20, 363-372 (1999b). https://doi.org/10.1016/S0731-7085(99)00065-5
  13. Sheng, Y.X., Li, L., Wang, Q., Guo, H.Z., and Guo, D.A., Simultaneous determination of gallic acid, albiflorin, paeoniflorin, ferulic acid and benzoic acid in Si-Wu decoction by high-performance liquid chromatography DAD method. J. Pharm. Biomed. Anal., 37, 805-810 (2005). https://doi.org/10.1016/j.jpba.2004.11.002
  14. Wang, S.Y., Zheng, W., and Galletta, G.J., Cultural system affects fruit quality and antioxidant capacity in strawberries. J. Agric. Food Chem. 50, 6534-6542 (2002). https://doi.org/10.1021/jf020614i
  15. Wang, Y.C. and Yang, Y.S., Simultaneous quantification of flavonoids and triterpenoids in licorice using HPLC. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 850, 392-399 (2007). https://doi.org/10.1016/j.jchromb.2006.12.032
  16. Xue, T.H. and Roy, R., Studying traditional Chinese medicine. Science, 300, 740-741 (2003).
  17. Zhang, H.J. and Cheng. Y.Y., An HPLC/MS method for identifying major constituents in the hypocholesterolemic extracts for Chinese medicine formula 'Xue-Fu-Zhu-Yu decoction'. Biomed. Chromatogr. 20, 821-826 (2006). https://doi.org/10.1002/bmc.607