Applications of Different Types of Germanium Compounds on Rice Plant Growth and its Ge Uptake

게르마늄 종류별 토양처리시 벼의 생육특성 및 게르마늄 흡수에 미치는 영향

  • Seo, Dong-Cheol (Department of Oceanography and Coastal Sciences, School of the Coast and Environment, Louisiana State University) ;
  • Cheon, Yeong-Seok (Division of Applied Life Science, GyeongSang National University) ;
  • Park, Seong-Kyu (Division of Applied Life Science, GyeongSang National University) ;
  • Park, Jong-Hwan (Division of Applied Life Science, GyeongSang National University) ;
  • Kim, Ah-Reum (Division of Applied Life Science, GyeongSang National University) ;
  • Lee, Won-Gyu (Department of Business Supprot, GyeongSangnamdo Provincial Government Building) ;
  • Lee, Seong-Tae (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Lee, Young-Han (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Cho, Ju-Sik (Division of Applied Life and Environmental Sciences, Sunchon National University) ;
  • Heo, Jong-Soo (Division of Applied Life Science, GyeongSang National University)
  • 서동철 (루이지애나주립대 해양연안과학과) ;
  • 천영석 (경상대학교 응용생명과학부) ;
  • 박성규 (경상대학교 응용생명과학부) ;
  • 박종환 (경상대학교 응용생명과학부) ;
  • 김아름 (경상대학교 응용생명과학부) ;
  • 이원규 (경상남도 도청 기업지원과) ;
  • 이성태 (경남농업기술원) ;
  • 이영한 (경남농업기술원) ;
  • 조주식 (순천대학교 생명환경과학부) ;
  • 허종수 (경상대학교 응용생명과학부)
  • Received : 2010.04.14
  • Accepted : 2010.04.16
  • Published : 2010.04.30

Abstract

In order to obtain the basic information for agricultural utilization of germanium (Ge), the growth characteristics, Ge uptake, and grain quality of rice plant (Hopyungbyeo) were investigated under different germanium ($GeO_2$, and commercial Ge) treatments in paddy field. Phytotoxicity was detected in $GeO_2$ treatment but not in commercial Ge treatment. The grain yield was greater in the order of control treatment > commercial Ge treatment > $GeO_2$ treatment. The dry weight was greater in order of control treatment > $GeO_2$ treatment ${\geq}$ commercial Ge treatment. The Ge content of leaf in $GeO_2$ treatment was 6 times (177 mg $m^{-2}$) higher than that in commercial Ge treatment. The Ge content in rice bran was not different in $GeO_2$, and commercial Ge treatments. The Ge contents of brown rice in$GeO_2$, and commercial treatments were 40.9, and 31.1 mg $kg^{-1}$, respectively. The Ge uptake rates in rice plant was higher in the order of leaf > rice bran > brown rice > stem > root. Under $GeO_2$, 15.56% of Ge absorbed into plant with 11.1% in leaf, 1.6% in stem, 0.03% in root, 2.2% in rice bran and 0.73% in brown rice. Under commercial Ge treatment, 5.19% of Ge absorbed into plant with 1.8% in leaf, 0.46% in stem, 0,01% in root, 2.2% in rice bran, and 0.71% in brown rice. Based on these results, the Ge contents in polished rice in commercial Ge treatment were higher than those in $GeO_2$ treatment. However, the Ge contents of rice grain (containing rice bran and polished rice) in $GeO_2$ treatment were higher than those in commercial Ge treatment.

게르마늄 토양처리시 게르마늄 종류가 벼의 생육특성 및 부위별 게르마늄 흡수에 미치는 영향을 조사하기 위해 무기게르마늄 ($GeO_2$)과 수도작용 액상게르마늄으로 시비를 달리하여 게르마늄 종류에 따른 벼의 생육특성, 게르마늄 종류에 따른 부위별 게르마늄 흡수 특성을 각각 조사한 결과 수도작용 액상게르마늄 처리구와 무처리구의 경우 벼에 게르마늄에 의한 독성이 거의 나타나지 않은 반면에 $GeO_2$ 처리구에서는 일부 벼에서 게르마늄의 독성이 나타났다. 게르마늄 종류에 따른 잎의 게르마늄 흡수량은 $GeO_2$ 처리구에서 평균 177.0 ${\mu}g\;m^{-2}$로 수도작용 액상게르마늄 처리구보다 약 6배 높았으나, 줄기와 뿌리의 게르마늄 흡수량은 게르마늄 종류에 따라 전반적으로 큰 차이는 없었다. 게르마늄 종류에 따른 쌀겨 중 게르마늄 함량은 $GeO_2$ 처리구 및 수도작용 액상 게르마늄 처리구 모두 별 차이 없이 비슷한 경향이었고, 현미 중 게르마늄 함량은 $GeO_2$ 처리구에서 평균 40.9 mg $kg^{-1}$으로 수도작용 액상게르마늄 처리구의 평균 31.1 mg $kg^{-1}$보다 유의성 있게 높았다. 하지만 백미 중 게르마늄 함량은 $GeO_2$ 처리구에서 평균 7.9 mg $kg^{-1}$으로 수도작용 액상게르마늄 처리구의 평균 14.3 mg $kg^{-1}$보다 유의성 있게 낮았다. 게르마늄 종류에 따른 벼 부위별 게르마늄 흡수율은 전반적으로 잎 > 쌀겨 > 현미(백미) > 줄기 > 뿌리 순으로 잎에서 가장 높았다. 쌀의 미질은 전반적으로 게르마늄 종류에 따라 별 차이 없었으나 무처리구에 비해 약간 낮은 경향을 나타내었다.

Keywords

References

  1. Aso, H., F. Suzuki, T. Yamaguchi, Y. Hayashi, T. Ebina, and N. Ishida. 1985. Induction of interferone and activation of NK cells and macrophages in mice by oral administration of Ge- 132, and organic germanium compound. Microbiol. Immunol. 29:65-74.
  2. Choi, J.C., and D.K. Cheon. 2002. Effect of harvest time on yield and quality of rice. Korean J. Crop Sci. 47:254-258.
  3. Dimartino, M.J. 1986. Antiarthritic and immunoregulatory activity of spirogermanium. J. Pharmacol. Exp. Ther. 236:103-110.
  4. Datnoff. L.E., G.H. Snyder, and G.H. Korndorfer. 2001. Silicon in agriculture. Elsevier Science.
  5. Ho, C.C., Y.F. Chem, and M.T. Lin. 1990. Effects of organogermanium compound 2-carboxyethylgermanium sesquioxide on cardiovascular function motor activity in rats. Pharmacology 41:286-291. https://doi.org/10.1159/000138736
  6. Hwang, S.H. 2008. Effect of organic and inorganic germanium on growth and its uptake of rice in paddy soil condition. Master Thesis. Gyeongsang National University of Education, Korea.
  7. Jang, B.C., and M.U. Park. 1997. Absorption and accumulation of Sr-85 by rice (Oryza sativa L.) and its transfer factor from soil to plant. Korea J. Soil science & Feritizer 30:184-188.
  8. Jang, J.J., K.J . Cho, Y.S. Lee, and J.H. Bae. 1991. Modifying responses of allyl sulfide, indole-3-carbinol and germanium in a rat multi-organ carcinogenesis model. Carcinogenesis 12:691-695. https://doi.org/10.1093/carcin/12.4.691
  9. Kehlbeck, H. 1983. New geramanium containing yeast for medicinal and veterinary use. Deutsch Patent DE. 3345211.
  10. Kwak, T.S., and J.H. Yeo. 2004. Variation of grain quality and grain filling rapidity Milyang 23 / Gihobyeo recombinant inbred lines. Korean J. Crop Sci. 49:160-166.
  11. Lee, H.M., and Y. Chung. 1991. Effect of organic germanium on metallothionnein induction in liver and kindey of cadmium and mercury intoxicated rats. Yakhak Hoeji 35:99-110.
  12. Lee, J.H., Y.S. Cho, M.T. Song, S.J. Yang, H.G. Hwang, N.S. Kim, H.C. Choi, and H.P. Moon. 2000. Analysis of quantitative Trait Loci (QTLs) related to rice gelatinization. Korean J. Breeding Sci. 32:211-217.
  13. Lee, M.S., J.H. Lee, T.O. Kwon, and S.B. Namkoong. 1995. Increment of germanium of concents in Angelica Keiskei Koidz and Panax ginseng C.A Meyer by in vitro propagation. Korean J. Medicinal Crop Sci. 3:251-258.
  14. Lee, S.T., Y.H. Lee, H.J. Lee, J.S. Cho, and J.S. Heo. 2005. Germanium contents of soil and crops in Gyeongnam Province. Korean J. Environ. Agric. 24:404-408. https://doi.org/10.5338/KJEA.2005.24.4.404
  15. Lee, S.T. 2004. Characteristics of growth response and germanium absorption of crops in soil treated germanium. Doctor Thesis. Gyeongsang National University of Education. Korea.
  16. Lim, JS., D.C. Seo, W.Y. Park, Y.S. Cheon, S.T. Lee, J.S. Cho, and J.S. Heo. 2008a. Effects of soil texture on germanium uptake and growth in rice by soil application with germanium. Korean J. Environ. Agric. 27:245-252. https://doi.org/10.5338/KJEA.2008.27.3.245
  17. Lim, J.S., D.C. Seo, W.Y. Park, Y.S. Cheon, S.K. Park, S.T. Lee, J.S. Cho, and J.S. Heo. 2008b. The selection of optimum rice species and germanium application method for production of functional rice with germanium. Korean J. Environ. Agric. 27:373-381. https://doi.org/10.5338/KJEA.2008.27.4.373
  18. Matsumoto, H., S. Syo, and E. Takahashi. 1975. Trans-location and some forms of germanium in rice plants. Soil Sci. Plant Nutr. 21:273-279. https://doi.org/10.1080/00380768.1975.10432642
  19. Mochizuki, H., and T. Kada. 1982. Antimutagenic effect of Ge-132 on $\gamma$-ray-induced mutation in Escherchia coli B/rWP2 trp-. Int. J. Radiat. Biol. 42:653-659. https://doi.org/10.1080/09553008214551621
  20. Obara, K., T. Saito, H. Sato, K. Yamakage, T. Watanabe, M. Kakizawa, T. Tsukamoto, K. Kobayashi, M. Hongo, and K. Yoshinaga. 1991. Germanium poisoning; clinical symptoms and renal damage caused by long-term intake of germanium. Japanese Jouranal of Medicine 30:67-72. https://doi.org/10.2169/internalmedicine1962.30.67
  21. Park, B.W., J.H. Lee, and T.O. Kwon. 1996. Effects of $GeO_{2}$ and citric acid on germanium content of callus and plant in Angelica koreana MAX. Korean J. Medicinal Crop Sci. 4:101-108.
  22. Rural Development Administration (RDA). 1988. Methods of soil chemical analysis. National Institute of Agriculture Science and Technology, Suwon (in Korea).
  23. Rural Development Administration (RDA). 1995. Research of farm in test investigation standard. National Institute of Agriculture Science and Technology, Suwon (in Korea).
  24. Suzuki, F., Brutkiewicz, R.R., and Pollard, R.B., 1986. Cooperation of lymphokine(s) and marcophages in expression of antitumor activity of carboxyethylgermanium (Ge-132). Antitumor Res. 62:177-182.
  25. Suzuki, Y., and K. Taguchi. 1983. Pharmacological studies of carboxyethylgermanium sesquioxide (Ge-132). Pharmacometrics 26:803-810.
  26. Wei, X. S. 1992. Effect of yeast on bioenrichment of germanium. Food Science. 149:49-54.