KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Preparation of Thiol-chitosan Coated EPA-containing Liposome and Immune Response in Mouse Model of Atopic Dermatitis

티올키토산으로 피복된 EPA(Eicosapentaenoic acid) 함유 리포좀의 제조 및 아토피 감염 쥐에 대한 면역 특성

  • Jung, Hyo-Yun (Interdisciplinary Program of Perfume and Cosmetics Graduate School, Chonnam National University) ;
  • Kim, Jin (Department of Advanced Chemicals & Engineering Graduate School, Chonnam National University) ;
  • Lee, Yong-Wook (Chemport Inc.) ;
  • Lee, Ki-Young (Interdisciplinary Program of Perfume and Cosmetics Graduate School, Chonnam National University)
  • 정효연 (전남대학교 공과대학 향장품학협동) ;
  • 김진 (전남대학교 공과대학 신화학소재공학과) ;
  • 이용욱 ((주)캠포트) ;
  • 이기영 (전남대학교 공과대학 향장품학협동)
  • Received : 2009.12.10
  • Accepted : 2010.01.03
  • Published : 2010.02.28
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Abstract

The enhancement of immunity for atopic dermatitis with application of eicosapentaenoic acid (EPA)-loaded liposome was evaluated on NC/Nga mice. The EPA-loaded liposome was coated with thiol-chitosan. The liposomes were characterized with transmission electron microscopy (TEM), surface zeta potential & particle size analyzer (Zeta-PSA) and differential scanning calorimetry (DSC). The loading efficiency of EPA in the liposome was about 4.7%. The particle size of the EPA-Ioaded liposome was about 230 nm. The values of Immunoglobulin E (IgE), interleukin-4 (IL-4), and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) were reduced significantly with application of the EPA-loaded liposome. The interferon-$\gamma$ (IFN-$\gamma$) value was increased with the application effect. It is concluded that EPA loaded liposome have immunity advancing effects in mouse model of atopic dermatitis.

Keywords

References

  1. Leng, D. Y. M., A. R. Rhodes, and R. S. Geha (2002) Atopic dermatits. pp. 1464-1480. In: T. B. Fitzpatrick, I. M. Freedberg, A. Z. Eisen, K. Wolff, K. F. Austen, L. A. Goldsmith, S. I. Katz. Dermatology in general medicine. 5th ed, McGraw-Hill Book, New York, USA.
  2. Ring, J., T. Bieber, D. Vieluf, B. Kunz, and B. Przybilla (1991) Atopic eczema Langerhans cells and allergy. InternationalArchives of Allergy and Applied Immunology. 94: 194-201. https://doi.org/10.1159/000235361
  3. Heusser, C. and P. Jardieu (1997) Therapeutic potential of anti-IgE antibodies. Current opinion in Immunology 9: 805-814. https://doi.org/10.1016/S0952-7915(97)80182-3
  4. Bos, J. D. and J. H. Sillevis Smitt (1996) Atopic dermatits. Journal of the Eurpean Academy of Dermatology and Venereology. 7: 101-114. https://doi.org/10.1111/j.1468-3083.1996.tb00605.x
  5. Borsonelo, E. C. and J. C. F. Galduroz (2008) The role of polyunsaturated fatty acids (PUFAs) in development. aging and substance abuse disorders. Prostaglandins, Leukotrienes and Essential Fatty Acids. 78: 237-245. https://doi.org/10.1016/j.plefa.2008.03.005
  6. Weldon, S. M., A. C. Mullen, C. E. Loscher, L. A. Hurley, and H. M. Roche (2007) Docosahexaenoic acid induces and anti-inflannnatory profile in lipopolysaccharide-stimulated human THP-1 marcophages more effectively than eicosapentaenoic acid. Journal of Nutritional Biochemistry. 18: 250-258. https://doi.org/10.1016/j.jnutbio.2006.04.003
  7. Jin, B. S. and S. H. Han (2002) Preparation and stability of Sonicated Liposome with Phospholipid Mixture. Dongduk Journal of Life Science Studies. 7: 134-139.
  8. Cho, E. C., H. J. Lim, J. W. Shim, J. O. Kim, and I. S. Chang (2007) Improved stability of liposome in oil/water emulsion by association of amphiphilic polymer with liposome and its effect on biioactive skin permeation. Colloid and Surfaces. 299: 160-168. https://doi.org/10.1016/j.colsurfa.2006.11.032
  9. Borchard, G., H. L. Lueben, A. G. Boer, J. C. Verhoef, C. M. Leur, and H. E. Junginger (1996) The potential of mucoadhesive polymers in enhancing intestinal peptide drug absorption. III: Effects of chitosan-glutamate and carbomer on epithelial tight jungciotions in vitro. Journal of Controlled Release. 39: 131-138. https://doi.org/10.1016/0168-3659(95)00146-8
  10. Margit, D. H., E. K. Constantia, and B. S. Andreas (2003) In vitro evaluation of the viscoelastic preperties of chitosan-thioglycolic acid conjugates. European Journal of Pharmaceutics and Biopharmaceutics. 55: 185-190. https://doi.org/10.1016/S0939-6411(02)00162-5
  11. Osuna, I., D. Teutonico, S. Arpicco, C. Vauthier, and G. Ponchel (2007) Characterization of chitosan thiolation and application to thiol quatification onto nanoparticle surface. International Journal of Pharmaceutics. 340: 173-181. https://doi.org/10.1016/j.ijpharm.2007.03.019
  12. Mills, L. B., L. J. Mordan, H. L. Roth, E. E. Winger, and W. L. Epstein (2000) Treatment of servere atopic dermatitis by topical immune modulation using dinitrochlorobenzene. Journal of the American academy of Dermatology. 42: 687-689. https://doi.org/10.1016/S0190-9622(00)90190-7
  13. Kast, C. E. and B. S. Andreas (2001) Thiolated polymers- Thiolmers: development and in vitro evaluation of chitosan-thioglycolic acid conjugates. Biomaterials. 22: 2345-2352. https://doi.org/10.1016/S0142-9612(00)00421-X
  14. Layre, A., R. Gref, J. Richard, D. Requier, H. Chacun, M. Appel, A. J. Domb, and P. Couvreur (2005) Nanoencapsulation of a crystalline drug. International Journal of Pharmaceutics. 298: 323-327. https://doi.org/10.1016/j.ijpharm.2005.02.039
  15. Schubring, R. (2009) Crystallisation and melting behaviour of fish oil measured by DSC. Journal of Thernal Analysis and Calorimetry. 95: 823-830. https://doi.org/10.1007/s10973-008-9418-z
  16. Kim, D. H., K. J. Lee, X. F. Qi, Y. M. Lee, Y. S. Yoon, J. L. Kim, B. S. Chang, and Y. S. Ryang (2008) The effects of magnesium rich sea mineral water on atopic dermatitis-like skin lesions in hairless mice. Korean Journal of Microscopy. 38: 167-174.
  17. Grewe, M., C. A. B. Koomen, E. Schpf, T. Thepen, A. G. L. Wildschut, T. Ruzicka, and J. Krutmann (1998) A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis. Immunology Today. 19: 359-361. https://doi.org/10.1016/S0167-5699(98)01285-7
  18. Jujo, K., H. Renz, J. Abe, E. W. Gelfand, and D. Y. Leung (1992) Decreased interferon $\gamma$ and increased interlukin-4 production in atopic dermatitis promotes IgE synthesis. Journal of Allergy and Clinical Immunology. 90: 323-331. https://doi.org/10.1016/S0091-6749(05)80010-7
  19. Renz, H., K. Jujo, J. Bradley, J. Dementico, E. W. Gelfand, and D. V. Leung (1992) Enhanced IL-4 production and IL-4 receptor expression in atopic dermatitis and their modulation by interferon-$\gamma$. Journal of Investigative Dermatology. 99: 403-408. https://doi.org/10.1111/1523-1747.ep12616114
  20. Hong, S., J. Y. Shim, J. K. Park, B. Yoo, and H. B. Moon (1997) Cytokine production of peripheral blood mononuclear cells from atopic asthmatics. Korean Journal of Allergy. 17: 307-315.
  21. Park, S. N., H. S. Jeong, and J. Y. Lee (2004) Effect of sopungsan in BALB/C mouse model of atopic dermatitis. Journal of Kyung Hee University Medical Center. 20: 129-141.
  22. Cho, S. H., S. H. Song, Y. K. Kim, Y. K. Jee, and Y. Y. Kim (1995) Regulation of IgE production in patients with atopic disease: Imbalance of IL-4 and IFN-$\gamma$ production. Journal of Allergy. 15: 205-215.