The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Genome Wide Expression Analysis of the Restored Changes by Carthami Flos Extract Treatment on Rat Brain Injury

흰쥐의 손상된 뇌조직에서의 유전자 발현 변화에 대한 홍화(紅花) 추출물 투여의 작용

  • Kim, Bu-Yeo (Laboratory of Radiation Molecular Cancer, Korea Institute of Radiological and Medical Sciences) ;
  • Limb, Se-Hyun (College of Nursing, Korea University) ;
  • Lee, Guem-San (School of Korean Medicine, Pu-San National University) ;
  • Kim, Hyung-Woo (School of Korean Medicine, Pu-San National University) ;
  • Lim, Chi-Yeon (Dept. of Medicine, Graduate School, Dong-Guk University) ;
  • Cho, Su-In (School of Korean Medicine, Pu-San National University)
  • 김부여 (한국원자력의학원 분자암연구실) ;
  • 임세현 (고려대학교 간호대학) ;
  • 이금산 (부산대학교 한의학전문대학원) ;
  • 김형우 (부산대학교 한의학전문대학원) ;
  • 임지연 (동국대학교 대학원 의학과) ;
  • 조수인 (부산대학교 한의학전문대학원)
  • Published : 2010.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The source is from the flower of Carthamus tinctorius L., family Compositae. It is used in clinical medicine to promote blood circulation, remove blood stasis, promote menstruation and alleviate pain. In the present study, we investigated the genome wide analysis of Carthami Flos on the intra-cranial hemorrhage(ICH) model. Methods : ICH in rat was induced by injection of collagenase type IV and Carthami Flos extract(CFe) was administered orally. The molecular profile of cerebral hemorrhage in rat brain tissue was measured using microarray technique to identify up- or down- regulated genes in brain tissue. Results : Expression profile showed that diverse genes were up- or down-regulated by ICH induction. Administration of CFe restored the expression level of some of altered genes by ICH to normal expressional level. Interestingly, these recovered genes by CFe were involved in the same biological pathways which were significantly activated or suppressed by ICH. Conclusion : The above results might explain the therapeutic mechanism of CFe on ICH. Further, by analyzing interaction network, core genes was identified which could be key molecular targets of CFe against ICH.

Keywords

References

  1. 통계청. 2008년 사망원인통계 결과. http://www.kostat.go.kr/nso_main/ nsoMainAction.do?method=search&catgrp=nso2009&catid=k09_0000&catid2=k09i_0000&forward=search.
  2. 尹吉榮. 東醫學의 客觀化와 東西醫學 病名 統一을 위한 方法. 東洋醫學. 1976;2(4):7-15.
  3. 康舜洙. 韓醫學에서의 瘀血에 대한 槪念. 大韓韓醫學會誌. 1984;5(1):138-40.
  4. 金完熙. 臟腑辨證論治. 서울: 成輔社; 1988, p. 59-61.
  5. 崔昇勳. 韓方病理學. 서울: 一中社; 1997, p. 70-4.
  6. 辛民敎 編著. 臨床本草學. 서울: 南山堂; 1997, p. 534-5.
  7. 全國韓醫科大學 本草學 敎授 共編著. 本草學. 서울: 永林社; 1994, p. 424-5.
  8. 申佶求. 申氏本草學 各論. 서울: 壽文社.; 1988, p. 554-6.
  9. 김동환, 이경섭, 송병기. 도인 및 홍화 약침의 진통, 항혈전 효능에 관한 연구. 대한한방부인과학회지. 2000;13(2):60-73.
  10. 임세현, 손영수, 백진웅, 조수인, 김영균. 紅花추출물 투여에 의한 뇌출혈 흰쥐 뇌조직의 유전자 발현 조절. 대한예방한의학회지. 2008;12(3):81-90.
  11. Beheshti B, Braude I, Marrano P, Thorner P, Zielenska M and Squire JA. Chromosomal localization of DNA amplifications in neuroblastoma tumors using cDNA microarray comparative genomic hybridization. Neoplasia. 2003;5(1):53-62.
  12. Tarca AL, Draghici S, Khatri P, Hassan SS, Mittal P, Kim JS, et al. A novel signaling pathway impact analysis. Bioinformatics. 2009;25:75-82. https://doi.org/10.1093/bioinformatics/btn577
  13. Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000;28:27-30. https://doi.org/10.1093/nar/28.1.27
  14. Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 2003;13:2498-504. https://doi.org/10.1101/gr.1239303
  15. Thora BH, Martine V, Eline L and Marieke S. Educational needs of patients with a stroke and their caregivers: A systematic review of the literature. Article in Press. http://dx.doi.org/10.1016/j.pec.2010.07.046.
  16. Ranga K and Krishnan R. Biological risk factors in late life depression. Biological Psychiatry. 2002;52(3):185-92. https://doi.org/10.1016/S0006-3223(02)01349-5
  17. Andrew IS and Robert IH. The role of platelets in thrombotic and vascular disease. Progress in Cardiovascular Diseases. 1979;22(1):31-52. https://doi.org/10.1016/0033-0620(79)90002-1
  18. 정동화, 안호진, 황규선, 윤기붕, 김태우, 문장혁 등. 홍화 약침의 견비통에 대한 임상적 연구. 대한침구학회지. 2002;19(6):184-92.
  19. 김효은, 강영화, 조명제, 김태우, 김은영, 나창수. 연부조직 손상으로 인한 경항통 환자의 홍화 약침 치료에 관한 임상적 고찰. 대한침구학회지. 2002;19(4):112-23.
  20. 기성식, 길기정, 이영종. 홍화와 익모초가 $CCl_4$로 손상된 흰쥐의 간에 미치는 영향. 대한본초학회지. 2001;16(2):79-89.
  21. 신윤숙, 손경희, 류동일. 홍화 황색소를 이용한 모염색의 최적화. 한국의류학회지. 2009;33(12):1971-8.
  22. 차원미, 김한식. 홍화를 이용한 모발의 천연염색. 대한미용과학회지. 2008;4(2):177-82.