YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Gardenia jasminoides Prevents Galactosamine-Induced Acute Hepatitis in Rats

Galactosamine 유도 급성 간염 모델에서 치자의 간 보호 효과

  • Received : 2010.07.16
  • Accepted : 2010.10.04
  • Published : 2010.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Gardenia jasminoides is a popular traditional herb used to treat inflammatory diseases including liver disorders. This study was performed to examine protective effect of G. jasminoides on galactosamine (GalN)-induced acute hepatitis. Rats were treated intraperitoneally with GalN (700 mg/kg). G. jasminoides (30, 100 and 300 mg/kg) was administered orally 48, 24, and 2 h before and 6 h after GalN injection. Serum ALT and AST activities were significantly increased after GalN injection, and these increases were attenuated by G. jasminoides. Histological studies showed that G. jasminoides inhibited hepatocellular necrosis with inflammatory cell infiltration. GalN decreased the serum levels of total cholesterol and this decrease was attenuated by G. jasminoides. Hepatic glutathione content was decreased and lipid peroxidation was increased after GalN treatment and these changes were attenuated by G. jasminoides. Furthermore, the level of tumor necrosis factor-${\alpha}$ mRNA expression was significantly increased after GalN injection, and this increase was attenuated by G. jasminoides. The level of interleukin-10 mRNA expression was significantly increased after GalN injection, and this increase was augmented by G. jasminoides. Our results suggest that G. jasminoides ameliorates GalN-induced acute hepatitis and this protection is likely due to antioxidative activity and regulation of inflammatory mediators.

Keywords

References

  1. Shin, J. K., Kim, H. Y. and Lee, S. M. : Protective effect of Gardenia jasminoides against carbon tetrachloride-induced acute hepatotoxicity. J. Pharm. Soc. Korea 54, 55 (2010). https://doi.org/10.5012/jkcs.2010.54.01.055
  2. Jaeschke, H., Smith, C. V. and Mitchell, J. R. : Reactive oxygen species during ischemia-reflow injury in isolated perfused rat liver. J. Clin. Invest. 81, 1240 (1988). https://doi.org/10.1172/JCI113441
  3. Bremer, C., Bradford, B. U., Hunt, K. J., Knecht, K. T., Connor, H. D., Mason, R. P. and Thurman, R. G. : Role of Kupffer cells in the pathogenesis of hepatic reperfusion injury. Am. J. Physiol. 267, G630 (1994).
  4. Loguercio, C. and Federico, A. : Oxidative stress in viral and alcoholic hepatitis. Free Radic. Biol. Med. 34, 1 (2003). https://doi.org/10.1016/S0891-5849(02)01167-X
  5. Keppler, D., Lesch, R., Reutter, W. and Decker, K. : Experimental hepatitis induced by D-galactosamine. Exp. Mol. Pathol. 9, 279 (1968). https://doi.org/10.1016/0014-4800(68)90042-7
  6. Farber, J. L., Gill, G. and Konishi, Y. : Prevention of galactosamine-induced liver cell necrosis by uridine. Am. J. Pathol. 72, 53 (1973).
  7. Decker, K. and Keppler, D. : Galactosamine hepatitis: key role of the nucleotide deficiency period in the pathogenesis of cell injury and cell death. Rev. Physiol. Biochem. Pharmacol. 71, 77 (1974). https://doi.org/10.1007/BFb0027661
  8. Pushpavalli, G., Kalaiarasi, P., Veeramani, C. and Pugalendi, K. V. : Effect of chrysin on hepatoprotective and antioxidant status in D-galactosamine-induced hepatitis in rats. Eur. J. Pharmacol. 631, 36 (2010). https://doi.org/10.1016/j.ejphar.2009.12.031
  9. Jaishree, V. and Badami, S. : Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against dgalactosamine induced acute liver damage in rats. J. Ethnopharmacol. 130, 103 (2010). https://doi.org/10.1016/j.jep.2010.04.019
  10. Lin, B. R., Yu, C. J., Chen, W. C., Lee, H. S., Chang, H. M., Lee, Y. C., Chien, C. T. and Chen, C. F. : Green tea extract supplement reduces D-galactosamine-induced acute liver injury by inhibition of apoptotic and proinflammatory signaling. J. Biomed. Sci. 16, 35 (2009). https://doi.org/10.1186/1423-0127-16-35
  11. Shin, J. W. and Park, N. H. : Treatment of chronic hepatitis B. Korean J. Med. 77, 265 (2009).
  12. Wang, G. X., Ben, C. E. and Ye, B. K. : Reparative effects of biphenyl dimethyl dicarboxylate on experimental liver injury in rats with histochemical and electronmicroscopy study. Zhong Xi Yi Jie He Za Zhi. 8, 158 (1988).
  13. Akao, T., Kobashi, K. and Aburada, M. : Enzymic studies on the animal and intestinal bacterial metabolism of geniposide. Biol. Pharm. Bull. 17, 1573 (1994). https://doi.org/10.1248/bpb.17.1573
  14. Kawata, Y., Hattori, M., Akao, T., Kobashi, K. and Namba, T. : Formation of nitrogen-containing metabolites from geniposide and gardenoside by human intestinal bacteria. Planta Med. 57, 536 (1991). https://doi.org/10.1055/s-2006-960201
  15. Lee, D. U., Park, C. H., Kang, S. I., Min, E. G., Han, Y. H. and Lee, C. K. : Isolation of the component transformed into blue pigments by aerobic bacteria in the fruits of gardenia jasmoides. Kor. J. Pharmacogn. 29, 204 (1998).
  16. 생약학교재편찬위원회. 생약학 동명사 1판, 420 (2006).
  17. Choi, S. J., Kim, M. J., Heo, H. J., Hong, B., Cho, H. Y., Kim, Y. J., Kim, H. K., Lim, S. T., Jun, W. J., Kim, E. K. and Shin, D. H. : Ameliorating effect of Gardenia jasminoides extract on amyloid beta peptide-induced neuronal cell deficit. Mol. Cells 24, 113 (2007).
  18. Hwang, S. M., Lee, Y. J., Yoon, J. J., Lee, S. M., Kang, D. G. and Lee, H. S. : Gardenia jasminoides inhibits tumor necrosis factor-alpha-induced vascular inflammation in endothelial cells. Phytother. Res. 24, S214 (2010). https://doi.org/10.1002/ptr.3099
  19. Auer, T., Khoschsorur, G. A., Rabl, H., Iberer, F., Petutschnigg, B., Wasler, A. and Tscheliessnigg, K. H. : Detection of lipid peroxidation products by malondialdehyde (MDA-TBA reaction) in organ transplantation. Transplant. Proc. 27, 2749 (1995).
  20. Al-Hesayen, A., Azevedo, E. R., Floras, J. S., Hollingshead, S., Lopaschuk, G. D. and Parker, J. D. : Selective versus nonselective beta-adrenergic receptor blockade in chronic heart failure: differential effects on myocardial energy substrate utilization. Eur. J. Heart Fail. 7, 618 (2005). https://doi.org/10.1016/j.ejheart.2004.04.015
  21. Griffith, O. W. : Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106, 207 (1980). https://doi.org/10.1016/0003-2697(80)90139-6
  22. Coen, M., Want, E. J., Clayton, T. A., Rhode, C. M., Hong, Y. S., Keun, H. C., Cantor, G. H., Metz, A. L., Robertson, D. G., Reily, M. D., Holmes, E., Lindon, J. C. and Nicholson, K. J. : Mechanistic aspects and novel biomarkers of responder and non-responder phenotypse in galactosamine-induced hepatitis. J. Proteome. Res. 8, 5175 (2009). https://doi.org/10.1021/pr9005266
  23. Ohkawa, H., Ohishi, N. and Yagi, K. : Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351 (1979). https://doi.org/10.1016/0003-2697(79)90738-3
  24. Prokopenko, V. M., Partsalis, G. K., Pavlova, N. G., Burmistrov, S. O. and Arutyunyan, A. V. : Glutathione-dependent system of antioxidant defense in the placenta in preterm delivery. Bull. Exp. Biol. Med. 133, 442 (2002). https://doi.org/10.1023/A:1019845217485
  25. Suresh, V. and Asha, V. V. : Preventive effect of ethanol extract of Phyllanthus rheedii Wight. on D-galactosamine induced hepatic damage in Wistar rats. J. Ethnopharmacol. 116, 447 (2008). https://doi.org/10.1016/j.jep.2007.12.011
  26. Blackwell, T. S. and Christman, J. W. : Sepsis and cytokines: current status. Br. J. Anaesth. 77, 110 (1996). https://doi.org/10.1093/bja/77.1.110
  27. Serracino-Inglott, F., Habib, N. A. and Mathie, R. T. : Hepatic ischemia-reperfusion injury. Am. J. Surg. 181, 160 (2001). https://doi.org/10.1016/S0002-9610(00)00573-0
  28. Abe, K., Ijiri, M., Suzuki, T., Taguchi, K., Koyama, Y. and Isemura, M. : Green tea with a high catechin content suppresses inflammatory cytokine expression in the galactosamine-injured rat liver. Biomed. Res. 26, 187 (2005). https://doi.org/10.2220/biomedres.26.187
  29. Opal, S. M. and DePalo, V. A. : Anti-inflammatory cytokines. Chest 117, 1162 (2000). https://doi.org/10.1378/chest.117.4.1162