Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of Sequence Diversity of mcyA Gene Involved in Microcystin Synthesis in Korean Reservoirs

국내 호수에서 Microcystins의 생합성에 관여하는 mcyA 유전자의 염기서열 다양성 분석

  • Oh, Kyoung-Hee (Department of Environmental Engineering, Chungbuk National University) ;
  • Han, Ah-Won (Department of Environmental Engineering, Chungbuk National University) ;
  • Cho, Young-Cheol (Department of Environmental Engineering, Chungbuk National University)
  • 오경희 (충북대학교 공과대학 환경공학과) ;
  • 한아원 (충북대학교 공과대학 환경공학과) ;
  • 조영철 (충북대학교 공과대학 환경공학과)
  • Received : 2010.04.09
  • Accepted : 2010.05.05
  • Published : 2010.06.30
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Abstract

The sequence diversity of mcyA gene involved in synthesis of microcystins was analyzed in Microcystis spp. isolated from the Korean reservoirs and in the environmental samples taken from the Daechung, Chungju, Yongdam, Soyang, and Euam Reservoirs at the cyanobacterial blooming season. It was estimated that the sequences of mcyA gene in the isolated Microcystis spp. were much conserved when compared with those in GenBank database. A few kinds of clones were dominant in the investigated environmental samples, occupying 87 to 100% of total clones. No mcyA sequences originated from Anabaena spp. or Planktothrix spp. was found. These results indicated that microcystins are produced mainly by Microcystis spp. and the sequences of mcyA genes are much conserved in the investigated Korean reservoirs.

국내에서 분리된 독소생산 Microcystis 속과 조류 대발생 시기에 대청호, 충주호, 용담호, 소양호, 및 의암호에서 채취한 시료에서 조류 독소인 microcystins의 생합성에 관여하는 mcyA 유전자의 염기서열 다양성을 분석하였다. GenBank에 등록된 mcyA 염기서열과 비교한 결과, 국내 호소에서 분리된 Microcystis 속의 mcyA 유전자 염기서열은 매우 낮은 다양성을 나타내었다. 환경 시료 분석 결과, 2-3종류의 clone이 전체의 87-100%를 차지하였으며, Anabaena 속이나 Planktothrix 속의 mcyA 유전자와 유사한 염기서열은 발견되지 않았다. 이러한 결과로 볼 때, 대상 호소에서 microcystins는 주로 Microcystis 속에 의해 생산되며, 독소 생산에 관여하는 mcyA 유전자의 염기서열은 보존되어 있는 것으로 판단된다.

Keywords

References

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