Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Optimization of Culture Media for Enhanced Chitinase Production from a Novel Strain of Stenotrophomonas maltophilia Using Response Surface Methodology

  • Khan, Minhaj Ahmad (Department of Biochemistry, Faculty of Science, Hamdard University) ;
  • Hamid, Rifat (Department of Biochemistry, Faculty of Science, Hamdard University) ;
  • Ahmad, Mahboob (Department of Biochemistry, Faculty of Science, Hamdard University) ;
  • Abdin, M.Z. (Department of Biotechnology, Faculty of Science, Hamdard University) ;
  • Javed, Saleem (Department of Biochemistry, Faculty of Science, Hamdard University)
  • Received : 2009.09.27
  • Accepted : 2010.07.23
  • Published : 2010.11.28
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Abstract

Chitinase is one of the most important mycolytic enzymes with industrial significance. This enzyme is produced by a number of organisms including bacteria. In this study, we describe the optimization of media components with increased production of chitinase for the selected bacteria, Stenotrophomonas maltophilia, isolated from soil. Different components of the defined media responsible for influencing chitinase secretion by the bacterial isolate were screened using Plackett-Burman experimental design and were further optimized by Box-Behnken factorial design of response surface methodology in liquid culture. Maximum chitinase production was predicted in medium containing 4.94 g/l chitin, 5.56 g/l maltose, 0.62 g/l yeast extract, 1.33 g/l $KH_2PO_4$, and 0.65 g/l $MgSO_4{\cdot}7H_2O$ using response surface plots and the point prediction tool of the DESIGN EXPERT 7.1.6 (Stat-Ease, USA) software.

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References

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