Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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PCR-DGGE as a Supplemental Method Verifying Dominance of Culturable Microorganisms from Activated Sludge

  • Zhou, Sheng (College of Environmental Science and Engineering South China University of Technology, The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education) ;
  • Wei, Chaohai (College of Environmental Science and Engineering South China University of Technology, The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education) ;
  • Ke, Lin (College of Environmental Science and Engineering South China University of Technology, The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education) ;
  • Wu, Haizhen (School of Bioscience and Bioengineering, South China University of Technology)
  • Received : 2009.08.13
  • Accepted : 2010.08.04
  • Published : 2010.11.28
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Abstract

To verify the dominance of microorganisms in wastewater biological treatment, PCR-DGGE (denaturing gradient gel electrophoresis) was performed as a supplementary support method for screening of the dominant microorganisms from activated sludge. Results suggest that the dominant microorganisms in activated sludge are primarily responsible for strengthening its effectiveness as a biological treatment system, followed by the non-main dominant microorganisms, whereas the non-dominant microorganisms showed no effects. The degree of microbial abundance present on the profile of PCR-DGGE was in line with the treatment efficiency of augmented activated sludge with isolated cultures, suggesting that PCR-DGGE can be used as an effective supplementary method for verifying culturable dominant microorganisms in activated sludge of coking wastewater.

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