Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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An Antiproliferative Ribonuclease from Fruiting Bodies of the Wild Mushroom Russula delica

  • Zhao, Shuang (State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University) ;
  • Zhao, Yong Chang (Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science) ;
  • Li, Shu Hong (Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science) ;
  • Zhang, Guo Qing (State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University) ;
  • Wang, He Xiang (State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University) ;
  • Ng, Tzi Bun (School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong)
  • Received : 2009.11.20
  • Accepted : 2009.12.25
  • Published : 2010.04.28
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Abstract

An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and $60^{\circ}C$, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C> poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an $IC_50$ value of $8.6\;{\mu}M$ and $7.2\;{\mu}M$, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.

Keywords

References

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