Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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NTAㆍNi2+-Functionalized Quantum Dots for VAMP2 Labeling in Live Cells

  • Received : 2010.03.08
  • Accepted : 2010.04.08
  • Published : 2010.06.20
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Abstract

An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag ($His_6$-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) ($NTA{\cdot}Ni^{2+}$) on the QD surfaces. We showed that the $NTA{\cdot}Ni^{2+}$-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted $His_6$-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.

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References

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