Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functional Characteristics and Diversity of a Novel Lignocelluloses Degrading Composite Microbial System with High Xylanase Activity

  • Guo, Peng (College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University) ;
  • Zhu, Wanbin (College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University) ;
  • Wang, Hui (College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University) ;
  • Lu, Yucai (College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University) ;
  • Wang, Xiaofen (College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University) ;
  • Zheng, Dan (Guangxi Soil and Fertilizer Station) ;
  • Cui, Zongjun (College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University)
  • Published : 2010.02.28
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Abstract

To obtain an efficient natural lignocellulolytic complex enzyme, we screened an efficient lignocellulose-degrading composite microbial system (XDC-2) from composted agricultural and animal wastes amended soil following a long-term directed acclimation. Not only could the XDC-2 degrade natural lignocelluloses, but it could also secrete extracellular xylanase efficiently in liquid culture under static conditions at room temperature. The XDC-2 degraded rice straw by 60.3% after fermentation for 15 days. Hemicelluloses were decomposed effectively, whereas the extracellular xylanase activity was dominant with an activity of 8.357 U/ml on day 6 of the fermentation period. The extracellular crude enzyme noticeably hydrolyzed natural lignocelluloses. The optimum temperature and pH for the xylanase activity were $40^{\circ}C$ and 6.0. However, the xylanase was activated in a wide pH range of 3.0-10.0, and retained more than 80% of its activity at $25-35^{\circ}C$ and pH 5.0-8.0 after three days of incubation in liquid culture under static conditions. PCR-DGGE analysis of successive subcultures indicated that the XDC-2 was structurally stable over long-term restricted and directed cultivation. Analysis of the 168 rRNA gene clone library showed that the XDC-2 was mainly composed of mesophilic bacteria related to the genera Clostridium, Bacteroides, Alcaligenes, Pseudomonas, etc. Our results offer a new approach to exploring efficient lignocellulolytic enzymes by constructing a high-performance composite microbial system with synergistic complex enzymes.

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