Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Cheonjeongkibo-Dan UV-Induced Cellular Damage in Human Dermal Fibroblast

천정기보단(天精氣保丹)의 자외선에 의한 세포 손상 억제 효과

  • Lee, Ghang-Tai (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Park, Si-Jun (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Lee, Jung-No (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Lee, Kwang-Sik (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Kim, Dae-Sung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Kun-Kuk (Coreana Cosmetics Ltd, SongPa R&D Center) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 이강태 (코리아나화장품 송파기술연구소) ;
  • 박시준 (코리아나화장품 송파기술연구소) ;
  • 이정로 (코리아나화장품 송파기술연구소) ;
  • 이광식 (코리아나화장품 송파기술연구소) ;
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 이건국 (코리아나화장품 송파기술연구소) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Received : 2010.11.06
  • Accepted : 2010.12.09
  • Published : 2010.12.25
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Abstract

In this study, we prepared CheonJeongKiBo-Dan(7 oriental medicinal plants, 7OMP: Astragalus Membranaceus root, Panax Ginseng root, Glycyrrhiza Glabra (licorice) root, Schizandra Chinensis fruit, Polygonatum Odoratum, Rehmannia Glutinosa root, Paeonia Albiflora root) by extracting them in one reactor and studied its efficacies on skin. UV irradiation has been suggested as a major cause of photoaging in skin. In order to investigate protective effects against UV-B induced cellular damage, 7OMP was extracted with 70% ethanol and dissolved in DMSO. The protective effect was detected by MTT assay, reactive oxygen species (ROS) generation, phosphorylation of ATR and p53 in human dermal fibroblast cell system after UV-B irradiation. 7OMP reduced UV-B-induced cellular damage in HDFs cells, and inhibited ROS generation. UV-B-induced toxicity accompanying ROS production and the resultant DNA damage are responsible for activation of ATR, p53 and Bad. In this study, 7OMP hampered phosphorylations of ATR and p53 in human dermal fibroblasts. Therefore, 7OMP may be protective against UV-induced skin photoaging.

Keywords

References

  1. Kang, S., Cho, S., Chung, J.H., Hammerberg, C., Fisher, G.J., Voorhees, J.J. Inflammation and extracellular matrix degradation mediated by activated transcription factors nuclear factor-kB and activator protein-1 in Inflammatory acne lesions in vivo. Am J. Pathol., 166: 1691-1699, 2005. https://doi.org/10.1016/S0002-9440(10)62479-0
  2. Matsumura, Y. and Ananthaswamy, H.N. Toxic effects of ultraviolet radiation on the skin. Toxicol. Appl. Pharmacol. 195: 298-308, 2004. https://doi.org/10.1016/j.taap.2003.08.019
  3. 全國韓醫科大學本草學敎授共編著. 本草學. 서울, 영림사, p535, 1995.
  4. 安德均. 韓國本草圖鑑. 서울, 도서출판 교학사, pp 644-645, 1998.
  5. Park, Y.J., Ho, Y., Shu, H.S., Shin, J.W., Lee, S.K. Distribution of Medicinal Constituents and content Korean J. Bread. 25: 146-150, 2003
  6. 辛民敎. 臨床本草學. 서울, 永林社, pp 172-173, 1997.
  7. Ohtaki, Y., Hida, T., Hiramatsu, K., Kanitani, M., Ohshima, T., Nomura, M., Wakita, H., Aburada, M., Miyamoto, K.I. Deoxycholic acid as an endogenous risk factor for hepatocarcinogenesis and effects of gomisin A, a lignan component of Schizandra fruits. Anticancer Res. 16: 751-755, 1996.
  8. Nishiyama, N., Chu, P.J., Saito, H. A herbal prescription, S113m, consisting and Schizandra, improves leaming performance in senescence accelerated mouse. Biol pharm Bull. 19: 388-393, 1996. https://doi.org/10.1248/bpb.19.388
  9. Blosis, M.S. Antioxidant determination by the use a stable free radical. Nature 26: 1199-1200, 1958.
  10. Mosmann, T. Rapid colormetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay. J Immun Methods. 65: 55, 1983. https://doi.org/10.1016/0022-1759(83)90303-4
  11. Dean, R.T., Fu, S., Stocker, R., Davies, M.J. Biochemistry and pathology of radical-mediated protein oxidation. Biochem J. 324: 1-18, 1997. https://doi.org/10.1042/bj3240001
  12. 김은정, 양기숙. 黃芪의 저밀도지질단백질 산화에 미치는 영향. 약학회지 45: 529-535, 2001.
  13. 김은정, 오오진, 이상국, 양기숙. 黃芪의 COX-2 활성 억제 효과. 생약학회지 32: 311-315, 2001.
  14. 김은정, 양기숙. 黃芪의 지질과산화 억제작용. 약학회지 49:11-19, 2005.
  15. 이진영, 박혜윤, 염명훈, 김덕희, 김한곤. 黃芪의 자외선에 의한 세포 손상을 막는 보호 효과. 생약학회지 39: 300-304, 2008.
  16. Hasegawa, H. Proof of the mysterious efficacy of ginseng: basic and clinical trials: metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid. J Pharmacol Sci. 95: 153-157, 2004. https://doi.org/10.1254/jphs.FMJ04001X4
  17. See, D.M., Broumand, N., Sahl, L., Tilles, J.G. In vitro effects of echinacea and ginseng on natural killer and antibody-dependent cell cytotoxicity in healthy subjects and chronic fatigue syndrome or acquired immunodeficiency syndrome patients. Immunopharmacology. 35: 229-235, 1997. https://doi.org/10.1016/S0162-3109(96)00125-7
  18. Park, E.K., Choo, M.K., Kim, E.J., Han, M.J., Kim, D.H. Antiallergic activity of ginsenoside Rh2. Biol pharm Bull. 26: 1581-1584, 2003. https://doi.org/10.1248/bpb.26.1581
  19. Lee, Y., Jin, Y., Lim, W., Ji, S., Choi, S., Jang, S., Lee, S. A ginsenoside-Rh1, a component of ginseng saponin, activates estrogen receptor in human breast carcinoma MCF-7 cells. J Steroid Biochem Mol Biol. 84: 463-468, 2003. https://doi.org/10.1016/S0960-0760(03)00067-0
  20. Liu, Z.Q., Luo, X.Y., Liu, G.Z., Chen, Y.P., Wang, Z.C., Sun, Y.X. In vitro study of the relationship between the structure of ginsenoside and its antioxidative or prooxidative activity in free radical induced hemolysis of human erythrocytes. J Agric Food Chem. 51: 2555-2558, 2003. https://doi.org/10.1021/jf026228i
  21. Kim, D.H., Moon, Y.S., Jung, J.S., Min, S.K., Son, B.K., Suh, H.W., Song, D.K. Effects of ginseng saponin administered intraperitoneally on the hypothalamo-pituitary-adrenal axis in mice. Neurosci Lett. 343: 62-66, 2003. https://doi.org/10.1016/S0304-3940(03)00300-8
  22. Dey, L., Xie, J.T., Wang, A., Wu, J., Maleckar, S.A., Yuan, C.S. Anti-hyperglycemic effects of ginseng: comparison between root and berry. Phytomedicine. 10: 600-605, 2003. https://doi.org/10.1078/094471103322331908
  23. Kim, N.D., Kim, E.M., Kang, K.W., Cho, M.K., Choi, S.Y., Kim, S.G. Ginsenoside Rg3 inhibits phenylephrine-induced vascular contraction through induction of nitric oxide synthase. Br J Pharmacol. 140: 661-670, 2003. https://doi.org/10.1038/sj.bjp.0705490
  24. Kim, S.H., Park, K.S. Effects of panax ginseng extract on lipid metabolism in humans. Pharmacol Res. 48: 511-513, 2003. https://doi.org/10.1016/S1043-6618(03)00189-0
  25. Hong, B., Ji, Y.H., Hong, J.H., Nam, K.Y., Ahn, T.Y. A double-blind crossover study evaluating the efficacy of korean red ginseng in patients with erectile dysfunction: a preliminary report. J Urol. 168: 2070-2073, 2002. https://doi.org/10.1016/S0022-5347(05)64298-X
  26. Kwon, J., Lee, S.J., SO, J.N., Oh, C.H. Effects of Schizandra chinensis fructus on the immunoregulatory action and apoptosis of L1210 cells. Korean J Food Sci Technol. pp 384-388, 2001.
  27. Akhtar, M.S., Ali, M.R. Study of hypoglycaemic activity of Cuminum nigrum seeds in normal and alloxan diabetic rabbits. Planta Med. 51: 81-85, 1985. https://doi.org/10.1055/s-2007-969411
  28. Zhao, Z., Wang, W., Guo, H., Zhou, D. antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats. Behav Brain Res. 194: 108-113, 2008. https://doi.org/10.1016/j.bbr.2008.06.030
  29. Fukai, T., Satoh, K., Nomura, T., Sakagami, H. Preliminary evaluation of antinephritis and radical scavenging activities of glabridin from Glycyrrhiza glabra. Fitoterapia. 74: 624-629, 2003. https://doi.org/10.1016/S0367-326X(03)00164-3
  30. Matsumoto, T., Tanaka, M., Yamada, H., Cyong, J.C. Effect of licorice roots on carrageenan-induced decrease in immune complexes clearance in mice. J Ethnopharmacol. 53: 1-4, 1996. https://doi.org/10.1016/0378-8741(96)01415-8
  31. Fu, Y., Hsieh, T.C., Guo, J., Kunicki, J., Lee, M.Y.W.T., Darzymkiewicz, Z., Wu, J. licochalcone-A, a novel flavonoid isolated from licorice root (Glycyrrhiza glabra), causes G2 and late-G1 arrests in androgen -independent PC-3 prostae cancer cells. Biochem Biophys Res Commun. 322: 263-270, 2004. https://doi.org/10.1016/j.bbrc.2004.07.094
  32. 문연자, 김 진, 임난영, 이승연, 곽섭, 황충연, 우원홍. 감초물추출물의 멜라닌 형성 억제효과. 동의생리병리학회지 16: 1230-1235, 2002.