The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Antifungal Activity of Bacterial Strains isolated from Tidal Mudflat and Salted Seafood (traditional Jeotgal) Against Six Major Plant Pathogens

갯벌 및 젓갈에서 분리한 세균의 작물 주요 병원균에 대한 항균활성 효과 검정

  • Kim, Tack-Soo (Division of Applied Biologics and Chemistry, College of Agriculture and Life Science, Chungnam National Applied Microbiology Laboratory, Agricultural Microbiology Division, NAAS, RDA) ;
  • Lee, Ga-Hyung (Division of Applied Biologics and Chemistry, College of Agriculture and Life Science, Chungnam National Applied Microbiology Laboratory, Agricultural Microbiology Division, NAAS, RDA) ;
  • Kim, Gyun-Jang (Division of Applied Biologics and Chemistry, College of Agriculture and Life Science, Chungnam National Applied Microbiology Laboratory, Agricultural Microbiology Division, NAAS, RDA) ;
  • Lee, Se-Won (Division of Applied Biologics and Chemistry, College of Agriculture and Life Science, Chungnam National Applied Microbiology Laboratory, Agricultural Microbiology Division, NAAS, RDA) ;
  • Park, Kyung-Seok (Division of Applied Biologics and Chemistry, College of Agriculture and Life Science, Chungnam National Applied Microbiology Laboratory, Agricultural Microbiology Division, NAAS, RDA) ;
  • Park, Jin-Woo (Division of Applied Biologics and Chemistry, College of Agriculture and Life Science, Chungnam National Applied Microbiology Laboratory, Agricultural Microbiology Division, NAAS, RDA)
  • 김택수 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 이가형 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 김균장 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 이세원 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 박경석 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 박진우 (농촌진흥청 국립농업과학원 농업미생물팀)
  • Received : 2010.11.17
  • Accepted : 2010.12.04
  • Published : 2010.12.31
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Abstract

Many bacterial strains inhabit strong saline condition, such as tidal mudflat and salted seafoods, were identified and reported for the proposed protease activities and salt resistance; however antifungal activities against plant fungal pathogen have not well been studied until now. In this study, primary screening was performed for the isolation of promising strains against major plant pathogens like Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora capsici, Botrytis cineria, Collectotrichum acutatum and Pythium ultimum. Totally 423 bacterial strain were isolated from laboratory media which was based on different morphological characteristics and all the strains were dual cultured against major fungal pathogens on PDA, finally 40 strains were selected as antifungal bacterial strain and identified by fatty acid phylogenic difference analysis from MIDI shorlock gas chromatography system. As a result, antifungal strains from tidal mudflat were 10 species of 6 genus. Paenibacillus macerans was dominant species; 5 strains among the 17 isolates from tidal mudflat. Antifungal strains from salted seafoods were 7 species of 3 genus and Bacillus atrophaeus was dominant species; 12 strains among the 23 isolates from salted fishes.

다양한 세균 종이 갯벌이나 젓갈 등 염분이 많은 환경에서 서식하고 있으며, 이들에 대한 단백질 분해능과 염분내성에 관한 많은 보고가 있지만 상대적으로 이들 세균의 식물에 대한 항진균 활성에 대한 연구는 부족한 실정이다. 본 연구에서는 고염분의 환경에서 서식하는 세균의 식물 병원균에 대한 항균활성을 검정하고자 수행되었으며, Sclerotinia sclerotiornm, Fusarium oxysporum, Phytophthora capsici, Botrytis cineria, Collectotrichum acutatum, Pythium ultimum의 6종에 대한 항균활성 세균을 1차적으로 선발하였다. 갯벌토양과 젓갈에서 형태적인 특성에 따라 분리된 총 423 점의 세균균주 중 40균주가 항균활성 균주로 선발되었으며, MIDI shorlock gas chromatography system 균체 지방산분석에 의해 이들의 종을 동정하였다. 갯벌토양에서 분리된 세균은 6속 10종에 속하는 것으로 확인되었으며 이들 17점의 세균 중 Paenibacillus macerans가 5점으로 가장 많았다. 젓갈에서 분리된 세균은 3속 7종에 속하는 것으로 확인되었으며, 이들 23점의 세균 중 Bacillus atrophaeus가 12점으로 가장 많았다.

Keywords

References

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