Reproductive Efficiency and Characteristics of Cloned Miniature Piglets Produced from Domestic Commercial Gilts

  • You, Jin-Young (College of Veterinary Medicine, Kangwon National University) ;
  • Jeon, Yu-Byeol (College of Veterinary Medicine, Chungbuk National University) ;
  • Hyun, Sang-Hwan (College of Veterinary Medicine, Chungbuk National University) ;
  • Park, Soo-Bong (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Lee, Eun-Song (College of Veterinary Medicine, Kangwon National University)
  • 투고 : 2010.11.22
  • 심사 : 2010.11.30
  • 발행 : 2010.12.31

초록

The objective of this study was to examine the reproductive characteristics of cloned miniature piglets produced from surrogate domestic pigs. Somatic cell nuclear transfer (SCNT) miniature pig embryos were transferred into domestic pigs. As controls, domestic pigs of the same breed with surrogates for SCNT embryos and miniature pigs of the same breed with the somatic cell donor were bred by artificial insemination and natural mating, respectively. Surrogate domestic pigs that farrowed cloned miniature piglets had a significantly longer gestation length (118.1 days) than conventionally bred domestic (115.4 days) and miniature (115.5 days) pigs. Furthermore, the birth weight of cloned miniature piglets produced from domestic pigs (743 g) was significantly greater than that of miniature piglets produced by natural breeding (623 g). Also, cloned miniature piglets had a significantly lower weaning rate (49.7%) than conventionally produced domestic (91.5%) and miniature (100%) piglets. No differences were observed between female and male cloned piglets in gestation length, litter size, birth weight, or weaning rate. Our results demonstrate that gestation length is extended in domestic pigs that are transferred with SCNT miniature pig embryos and that cloned miniature piglets have increased birth weight and high pre-weaning mortality.

키워드

참고문헌

  1. Cho SK, Hwang KC, Choi YJ, Bui HT, Nguyen VT, Park C, Kim JH and Kim JH. 2009. Production of transgenic pigs harboring the human erythropoietin (hEPO) gene using somatic cell nuclear transfer. J. Reprod. Dev. 55:128-136. https://doi.org/10.1262/jrd.20102
  2. Hiendleder S, Mund C, Reichenbach HD, Wenigerkind H, Brem G, Zakhartchenko V, Lyko F and Wolf E. 2004. Tissuespecific elevated genomic cytosine methylation levels are associated with an overgrowth phenotype of bovine fetuses derived by in vitro techniques. Biol. Reprod. 71:217-223. https://doi.org/10.1095/biolreprod.103.026062
  3. Kennedy BW and Moxley JE. 1978. Genetic and environmental factors influencing litter size, sex ratio, and gestation length in the pig. Anim. Prod. 27:35-42. https://doi.org/10.1017/S0003356100035790
  4. Kolber-Simonds D, Lai L, Watt SR, Denaro M, Am S, Augenstein ML, Betthauser J, Carter DB, Greenstein JL, Hao Y, Im GS, Liu Z, Men GD, Murphy CN, Park KW, Rieke A, Ryan DJ, Sachs DH, Forsberg EJ, Prather RS and Hawley RJ. 2004. Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. Proc. Natl. Acad. Sci. USA 101:7335-7340. https://doi.org/10.1073/pnas.0307819101
  5. Lee SY, Park JY, Choi YJ, Cho SK, Ahn JD, Kwon DN, Hwang KC, Paik SS, Seo HG, Lee HT and Kim JH. 2007. Comparative proteomic analysis associated with term placental insufficiency in cloned pig. Proteomics 8:1303-1315.
  6. Norman HD, Wright JR, Kuhn MT, Hubbard SM, Cole JB and VanRaden PM. 2009. Genetic and environmental factors that affect gestation length in dairy cattle. J. Dairy Sci. 92:2259-2269. https://doi.org/10.3168/jds.2007-0982
  7. Nottle MB, Beebe LF, Harrison SJ, McIlfatrick SM, Ashman RJ, O'Connell PJ, Salvaris EJ, Fisicaro N, Pommey S, Cowan PJ and d'Apice AJ. 2007. Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer. Xenotransplantation 14:339-344. https://doi.org/10.1111/j.1399-3089.2007.00417.x
  8. Park KW, Cheong HT, Lai L, Im GS, Kuhholzer B, Bonk A, Samuel M, Rieke A, Day BN, Murphy CN, Carter DB and Prather RS. 2001. Production of nuclear transfer-derived swine that express the enhanced green fluorescent protein. Anim. Biotechnol. 12:173-181. https://doi.org/10.1081/ABIO-100108344
  9. Park MR, Cho SK, Lee SY, Choi YJ, Park JY, Kwon DN, Son WJ, Paik SS, Kim T, Han YM and Kim JH. 2005. A rare and often unrecognized cerebromeningitis and hemodynamic disorder: a major cause of sudden death in somatic cell cloned piglets. Proteomics 5:1928-1939. https://doi.org/10.1002/pmic.200401079
  10. Park Y, Hong J, Yong H, Lim J and Lee E. 2005. Effect of exogenous carbohydrates in a serum-free culture medium on the development of in vitro matured and fertilized porcine embryos. Zygote 13:269-275. https://doi.org/10.1017/S0967199405003369
  11. Pere MC, Dourmad JY and Etienne M. 1997. Effect of number of pig embryos in the uterus on their survival and development and on maternal metabolism. J. Anim. Sci. 75:1337-1342.
  12. Petters RM and Wells KD. 1993. Culture of pig embryos. J. Reprod. Fert. Suppl. 48:61-73.
  13. Rydhmer L, Lundeheim N and Canario L. 2008. Genetic correlations between gestation length, piglet survival and early growth. Livest. Sci. 115:287-293. https://doi.org/10.1016/j.livsci.2007.08.014
  14. Sasaki Y and Koketsu Y. 2007. Variability of gestation length across parity associated with reproductive performance in a cohort of gilts on commercial farms. Theriogenology 68:123-127. https://doi.org/10.1016/j.theriogenology.2007.04.021
  15. Song K, Hyun SH, Shin T and Lee E. 2009. Post-activation treatment with demecolcine improves development of somatic cell nuclear transfer embryos in pigs by modifying the remodeling of donor nuclei. Mol. Reprod. Dev. 76:611-619. https://doi.org/10.1002/mrd.20989
  16. Walker SC, Christenson RK, Ruiz RP, Reeves DE, Pratt SL, Arenivas F, Williams NE, Bruner BL and Polejaeva IA. 2007. Comparison of meat composition from offspring of cloned and conventionally produced boars. Theriogenology 67:178-184. https://doi.org/10.1016/j.theriogenology.2006.09.025
  17. Williams NE, Walker SC, Reeves DE, Sherrer E, Galvin JM, Polejaeva I, Rampacek G, Benyshek L, Christenson RK, Graves WM and Pratt SL. 2006. A comparison of reproductive characteristics of boars generated by somatic cell nuclear transfer to highly related conventionally produced boars. Cloning Stem Cells 8:130-139. https://doi.org/10.1089/clo.2006.8.130
  18. Young LE, Sinclair KD and Wilmut I. 1998. Large offspring syndrome in cattle and sheep. Rev. Reprod. 3:155-163. https://doi.org/10.1530/ror.0.0030155