Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
권호 표지

The Effect of Intermittent Compressive Loading to Growth of Pre-osteoblast Cells

간헐적인 압축하중이 조골세포주 성장에 미치는 영향

  • Choi, Sung-Kyu (Department of Precision Mechanical Engineering, Chungbuk National Univ.) ;
  • Park, Jeong-Hun (Department of Mechanical Engineering, POSTECH) ;
  • Lee, Seung-Jae (BK21 Mechatronics group, Chungnam Natioanl Univ.) ;
  • Lee, In-Hwan (School of Mechanicsal Engineering, Chungbuk National Univ.) ;
  • Kang, Sang-Sun (School of Science Education, Chungbuk National Univ.)
  • 최성규 (충북대학교 정밀기계공학과) ;
  • 박정훈 (포항공과대학교 기계공학과) ;
  • 이승재 (충남대학교 BK21 메카트로닉스 사업단) ;
  • 이인환 (충북대학교 기계공학부) ;
  • 강상순 (충북대학교 과학교육과)
  • Published : 2010.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, it has been reported that mechanical stimulation takes a role in improving cell growth. Also, became generally known that skeletal system as bone or cartilage tissues take influence of compression loading. In this study, we fabricated a custom-made bioreactor and analyzed that conditions of compressive loading would influence cell growth. To compare the effect of intermittent compressive loading on cell-encapsulated agarose scaffold, we cultured preosteoblast cell (MC3T3-E1 cells) statically and dynamically. And dynamic culture conditions were produced by changing parameters such as the iteration time and interval delay time. Also, cellencapsulated agarose scaffold were subjected to 10 % dynamic compressive strain at 1㎐ frequency for 7 days. After cell culture, cell proliferation was assessed with PI stain assay for fluorescence images and flow cytometry (FACS).

Keywords

References

  1. Mauck, R. L., Soltz, M. A., Wang, C. C., Wong, D. D., Chao, P. H., Valhmu, W. B., Hung, C. T. and Ateshian, G. A., "Functional Tissue Engineering of Articular Cartilage Through Dynamic Loading of Chondrocyte- Seeded Agarose Gels," Journal of Biochemical Engineering, Vol. 122, No. 3, pp. 252-260, 2000.
  2. Kisiday, J. D., Jin, M. S, DiMicco, M. A., Kurz, B. and Grodzinsky, A. J., "Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffold," Journal of Biomechanics, Vol. 37, No. 5, pp. 594-604, 2004.
  3. Meyer, U., Buchter, A., Nazer, N. and Wiesmann, H. P., "Design and performance of a bioreactor system for mechanically promoted three-dimensional tissue engineering," British Journal of Oral and Maxillofacial Surgery, Vol. 44, No. 2, pp. 134-140, 2006. https://doi.org/10.1016/j.bjoms.2005.05.001
  4. Engelmayr Jr., G. C., Hildebrand, D. K., Sutherland, F. W. H., Mayer Jr, J. E. and Sacks, M. S., "A novel bioreactor for the dynamic flexural stimulation of tissue engineered heart valve biomaterials," Biomaterials, Vol. 24, No. 14, pp. 2523-2532, 2003. https://doi.org/10.1016/S0142-9612(03)00051-6
  5. Preiss-Bloom, O., Mizrahi, J., Elisseeff, J. and Seliktar, D., "Real-time monitoring of force resp onse measured in mechanically stimulated tissueengineered cartilage," Artificial Organs, Vol. 33, No. 4, pp. 318-327, 2009. https://doi.org/10.1111/j.1525-1594.2009.00723.x
  6. Hunter, C. J., Mouw, J. K., Levenston, A. E., Coulter, W. H. and Woodruff, G. W., "Dynamic compression of chondrocyte-seeded fibrin gels: effects on matrix accumulation and mechanical stiffness," Osteo Arthritis and Cartilage, Vol. 12, No. 2, pp. 117-130, 2004. https://doi.org/10.1016/j.joca.2003.08.009
  7. Park, J. H., Lee, S.-J., Lee, I. H., Cho, D.-W. and Rhie, J.-W., "Development of bioreactor by rapid prototyping technology," Journal of the Korean Society for Precision Engineering, Vol. 26, No. 3, pp. 137-143, 2009.
  8. Nugent-Derfus, G. E., Takara, T., O'neill, J. K., Cahill, S. B., Gortz, S., Pong, T., Inoue, H., Aneloski, N. M., Wang, W. W., Vega, K. I., Klein, T. J., Hsieh- Bonassera, N. D., Bae, W. C., Bugbee, J. D. and Sah, R. L., "Continuous passive motion applied to whole joints stimulates chondrocyte biosynthesis of PRG4," Osteo Arthritisand Cartilage, Vol. 15, No. 5, pp. 556- 574, 2007.
  9. Bae, S.-M., Kyung, H.-M. and Sung, J.-H., "The effects of mechanical stress on alkaline phosphatase activity of MC3T3-E1 cells," The Korean Journal of Orthodontics, Vol. 26, No. 3, pp. 291-299, 1996.
  10. Choi, S.-Y. and Koh, K.-J., "The effects of irradiation on the mRNA expression of type I collagen and alkaline phosphatase in the MC3T3-E1 osteoblastic cell line," Korean Journal of Oral Maxillofac Radiol, Vol. 33, No. 1, pp. 51-57, 2003.
  11. Awad, H. A., Wickham, Q. W., Leddy, H. A., Gimble, J. M. and Guilak, F., "Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate and gelatin scaffolds," Biomaterials, Vol. 25, No. 16, pp. 3211-3222, 2004. https://doi.org/10.1016/j.biomaterials.2003.10.045
  12. Wikipedia, http://en.wikipedia.org/wiki/Western_blot
  13. Leboy, P. S., Beresford, J. N., Devlin, C. and Owen, M. E., "Dexamethasone induction of osteoblast mRNA in rat marrow stromal cell cultures," Journal of Cellular Physiology, Vol. 146, No. 3, pp. 370-378, 1991. https://doi.org/10.1002/jcp.1041460306