Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhancement of Ornithine Production in Proline-Supplemented Corynebacterium glutamicum by Ornithine Cyclodeaminase

  • Lee, Soo-Youn (Department of Bioprocess Engineering, Chonbuk National University) ;
  • Cho, Jae-Yong (Division of Animal Science and Biotechnology, Sangji University) ;
  • Lee, Hyun-Jeong (Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University) ;
  • Kim, Yang-Hoon (School of Life Science, Chungbuk National University) ;
  • Min, Ji-Ho (Department of Bioprocess Engineering, Chonbuk National University)
  • Published : 2010.01.31
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Abstract

In this study, Corynebacterium glutamicum and its derived mutants were used to demonstrate the relationship between proline, glutamate, and ornithine. The maximum ornithine production was shown in the culture medium (3,295.0 mg/l) when the cells were cultured with 20 mM proline, and was 15.5 times higher than in the presence of 1 mM proline. However, glutamate, which is known as an intermediate in the process of converting proline to ornithine, did not have any positive effect on ornithine production. This suggests that the conversion of proline to ornithine through glutamate, is not possible in C. glutamicum. Comparative analysis between the wild-type strain, SJC 8043 ($argF^-$, $argR^-$), and SJC 8064 ($argF^-$, $argR^-$, and $ocd^-$), showed that C glutamicum could regulate ornithine production by ornithine cyclodeaminase (Ocd) under proline-supplemented conditions. Therefore, proline directly caused an increase in the endogenous level of ornithine by Ocd, which would be a primary metabolite in the ornithine biosynthesis pathway.

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References

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