Journal of Microbiology and Biotechnology

  • 제20권1호
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  • Pages.110-116
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  • 2010
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Immobilization of Lactobacillus salivarius ATCC 11741 on Loofa Sponge Coated with Chitosan for Lactic Acid Fermentation

  • Chantawongvuti, R. (Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University) ;
  • Veerajetbodithat, J. (Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University) ;
  • Jaturapiree, P. (Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University) ;
  • Muangnapoh, C. (Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University)
  • 발행 : 2010.01.31
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초록

Lactic acid (LA) fermentation by Lactobacillus salivarius ATCC 11741 immobilized on loofa sponge (LS) was evaluated. To increase the surface area of LS for cell immobilization, $H_2O_2$ and chitosan were introduced as surface modifying reagents. Four chitosans of different molecular weights were separately coated on LS. All experiments were conducted in shaking flask mode at 100 rpm rotating speed and $37^{\circ}C$ with 5% $CaCO_3$ as a pH regulating agent. The effects of initial glucose concentration were investigated in the range of 20-100 g/l on LA fermentation by free cells. The results indicate that the maximum concentration of LA was produced with 50 g/l glucose concentration. The immobilized cell system produced 1.5 times higher concentration than free cells for 24 h of fermentation. Moreover, immobilized cells can shorten the fermentation time by 2-fold compared with free cells at the same level of LA concentration. At 1% (w/v) chitosan in 2% (v/v) acetic acid, the Yp/s and productivities of various molecular weights of chitosans were insignificantly different. Repeated batch fermentations showed 5 effective recycles with Yp/s and productivity in the range of 0.55-0.85 and 0.90-1.20 g/l.h, respectively. It is evident that immobilization of L. salivarius onto LS permits reuse of the system under these fermentation conditions. Scanning electron micrographs indicated that there were more intact cells on the chitosan-treated LS than on the untreated LS, thus confirming the effectiveness of the LS-chitosan combination when being utilized as a promising immobilization carrier for LA fermentation.

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