Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Optimal Enrichment Temperature for Rotifer and Microalgae Selection for Cold-Water Species Culture

한해성 품종 종묘생산을 위한 로티퍼의 적정 영양강화 수온 및 미세조류 선택

  • Park, Jin-Chul (National Fisheries Research and Development Institute) ;
  • Lee, Bae-Ik (National Fisheries Research and Development Institute) ;
  • Park, Heum-Gi (Faculty of Marine Bioscience & Technology, Gangneung-Wonju National University) ;
  • Kwon, O-Nam (Marine Biology Center for Research and Education, Gangneung-Wonju National University)
  • Received : 2010.08.12
  • Accepted : 2010.11.06
  • Published : 2010.12.31
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Abstract

The purpose of this study is to establish methods for enhancing the survival and growth of cold-water fish and crustacean larvae based on the nutritional components of zoo and phyto live foods. Rotifers, Brachionus rotundiformis, were cultured with a supplement of freshwater condensed Chlorella vulgaris at $28^{\circ}C$ and enriched with Algamac $2000^{(R)}$ at 16, 20 and $26^{\circ}C$, respectively. Isochrysis galbana, Chaetoceros calcitrans and Chlorella ellipsoidea were centrifuged for component analysis after being cultured for approximately one week with conway medium at $20^{\circ}C$. The crude protein and lipid contents of the rotifers were 58.4% and 10.9%, respectively, before enrichment. After enrichment at each temperature, total protein and essential amino acid contents were increased by reducing the enrichment temperature. However, unsaturated fatty acids and multiple fatty acid index (UI) showed their highest values at $20^{\circ}C$. Mono-unsaturated fatty acid content was highest (72.6%) at $16^{\circ}C$. The total protein contents of C. calcitrans and C. ellipsoidea were higher, 33.0% and 35.2%, respectively, than that of I. galbana, 27.8%. Methionin, leusine and histidine, essential amino acids of C. ellipsoidea, had considerably higher values, 50.2, 287.2 and 68.1 mg/g dry matter, respectively, compared to other microalgae. Total lipids, UI, DHA and n-3 PUFA of I. galbana had higher values, 23.6, 272.0, 12.9% and 45.2%, respectively, than other microalgae. Therefore, for cold-water fish and crustacean larvae that require high n-3 PUFA and DHA contents, enrichment of rotifers is desirable at $20^{\circ}C$. Fish larvae would also need more I. galbana than other microalgae.

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References

  1. Baskerville-Bridges B and Kling LJ. 2000, Larval culture of Atlantic cod (Gadus morhua) at high stocking densities. Aquaculture 181, 61-69. https://doi.org/10.1016/S0044-8486(99)00220-3
  2. Bolasina S, Prez A and Yamashita Y. 2006. Digestive enzymes activity during ontogenetic development and effect of starvation in Japanese flounder Paralichthys olivaceus. Aquaculture 252, 503-515. https://doi.org/10.1016/j.aquaculture.2005.07.015
  3. Brown MR. 2002. Nutritional value of microalgae for aquaculture. In: Cruz-Suaez LE, Ricque-Marie D, Tapia-Salazar M, Gaxiola-Corte MG, Simoes N, eds. Advances en Nutricio Acuicola VI. Memorias del VI Simposium International de Nutricion Acuicola. 3 al 6 de Septiembre del 2002, Cancu, Quintana Roo, Meico, 281-292.
  4. Cahu CL, Zambonino Infante JL, Pees A, Quazuguel P and Le Gall MM. 1998. Algal addition in sea bass (Dicentrarchus labrax)larvae rearing: effect on digestive enzymes. Aquaculture 161, 479-489. https://doi.org/10.1016/S0044-8486(97)00295-0
  5. Choi, JH, Hong BK, Jun YY, Kim JN, Choi YM and Yoo OH. 2009. Feeding comparison of three deep-sea fish, Lumpenella longirostris, Malacocottus gibber and Bothrocara hollandi, in the East Sea. J Kor Fish Soc 42, 151-156. https://doi.org/10.5657/kfas.2009.42.2.151
  6. Denis L, Desroy N and Ropert M. 2007. Ambient flow velocity and resulting clearance rates of the terebellid polychaete Lanicecon chilega (Pallas, 1766). J Sea Res 58, 209-219. https://doi.org/10.1016/j.seares.2007.03.005
  7. Duncan DB. 1955. Multiple-range and multiple F-tests. Biometrics 11, 1-42. https://doi.org/10.2307/3001478
  8. Flynn KJ, Zapata M, Garrido JL, Oik H and Hipkin CR. 1993. Changes in carbon and nitrogen physiology during ammonium and nitrate nutrition and nitrogen starvation in Isochrysis galbana. Eur J Physio 128, 47-52.
  9. Hagiwara A and Lee CS. 1991. Resting eggs formation of the L-type and S-type rotifer Brachionus plicatilis under different water temperature. Nippon Suisan Gakkaishi 57, 1645-1650. https://doi.org/10.2331/suisan.57.1645
  10. Hamasaki K. 2003. Effects of temperature on the egg incubation period, survival and developmental period of larvae of the mud crab Scylla serrata (Forskal) (Brachyura: Portunidae) reared in the laboratory. Aquaculture 219, 561-572. https://doi.org/10.1016/S0044-8486(02)00662-2
  11. Huervana FH, Joana JY and Cruz DL. 2006. Inhibition of luminous Vibrio harveyi byy "Green water" obtained from tank culture of tilapia, Oreochromis mossambicus. ACTA Ich Pisc 36, 17-23. https://doi.org/10.3750/AIP2006.36.1.03
  12. Hur SB and HG Park. 1996. Mass production of resting egg of Korean rotifer Brachionus plicatilis (LandStype). J Aquaculture 9, 345-351.
  13. Guderley H. 2004. Metabolic responses to low temperature in fish muscle. Biol Rev 79, 409-427.
  14. Kogane T, Hamasaki K and Dan S. 2007. Improvement of larval survival rates by water agitation and medication in the seed production of snow crab Chionecetes opilio. Nippon Suisan Gakkaishi 73, 226-232. https://doi.org/10.2331/suisan.73.226
  15. Kwon ON and Park HG. 2008. The optimal enrichment condition of rotifer Brachionus rotundiformis. J Aquacult 21, 41-46.
  16. Kwon ON, Park HG, Lee JH. 2003. Biochemical changes of a fish (sweet fish) cultured on the ordinary method. 2003 Abstract, Ann Meet Kor Soc Fish. Aquat Sci, 191-192.
  17. Malak NA, Brichon G. Meister R and Zwingelstein G. 1989. Environmental temperature and metabolism of the molecular species of phosphatidylcholine in the tissue of the Rainbow trout. Lipid 24, 318-324. https://doi.org/10.1007/BF02535170
  18. Meeren T, Mangor-Jensen A and Pickova J. 2007. The effect of green water an light intensity on survival, growth and lipid composition in Atlantic cod (Gadus morhua) during intensive larval rearing. Aquaculture 265, 206-217. https://doi.org/10.1016/j.aquaculture.2007.01.042
  19. Mercier L, Audet C, Noe J. Parent B, Parrish CC and Ross NW. 2004. First feeding of winter flounder (Pseudopleuronectes americanus) larvae: use of Brachionus plicatilis acclimated at low temperature as liveprey. Aquaculture 229, 361-376. https://doi.org/10.1016/S0044-8486(03)00399-5
  20. Park HH, Jeong EC, Bae BS, Yang YS, Hwang SJ, Park JH, Kim YS, Lee SI and Choi SH. 2007. Fishing investigation and species composition of the catches caught by a bottom trawl in the deep Eas Sea. J Kor Soc FIsh Tech 43, 183-191. https://doi.org/10.3796/KSFT.2007.43.3.183
  21. Park HG, Puvanendran V, Kellett A, Parrish CC and Brown JA. 2006. Effect of enriched rotifers on growth, survival, and composition of larval Atlantic cod (Gadus morhua). ICES J Marine Science 285-295.
  22. Parrish CC. 1987. Separation of aquatic lipid classes by Chromarod thin-layer chromatography with measurement by Iatroscan flame ionization detection. Can J Fish Aquat Sci 44, 722−d731.
  23. Pruitt NL. 1988. Membrane lipid composition and overwintering strategy in thermally acclimated crayfish. Am J Physiol Regul Integr Comp Physio 254, 870-876.
  24. Reitan KH, Kjorsvik E and Reitan KI. 2001. Development of the pH in the intestinal tract of larval turbot. Mar Biol 139, 1159-1164. https://doi.org/10.1007/s002270100653
  25. Reitan KI, Rainuzzo JR, Gunvor O and Olsen Y. 1997. A review of the nutritional effects of algae in marine fish larvae. Aquaculture 155, 207-221. https://doi.org/10.1016/S0044-8486(97)00118-X
  26. Tytler P, Ireland J and Murray L. 1997. A study of the assimilation of fluorescent pigment of microalgae Isochrysis galbana by the early larvae stages of turbot and herring. J Fish Biol 50, 999-1009.
  27. Vagner M, Robin JH, Zambonino Infante JL and Ruyet JP. 2007. Combined effects of dietary HUFA level and temperature on sea bass (Dicentraechus labrax) larvae development. Aquaculture 266, 179-190. https://doi.org/10.1016/j.aquaculture.2007.02.040
  28. Watanabe Y, Yamada N, Machida T, Honjoh K and Kuwano E. 2009. Influence of cold hardening on chlorophyll and carotenoid in Chlorella vulgaris. J Fac Agr Kyushu Univ 54, 195-200.
  29. Zapata M and Garrido JL. 1996. Occurrence of phyiwated chlorophyll c in Isochrysis galbana and Isochrysis sp. (clone T-Iso) (Prymnesiophyceae). J Phyco 133, 209-214.