Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Inhibitory Effects of Sargassum thunbergii Ethanol Extract against α-amylase

지충이 에탄올 추출물의 α-amylase 저해활성

  • Lee, So-Jeong (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Song, Eu-Jin (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Lee, Chung-Jo (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Jung, Ji-Yeon (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Kwak, Ji-Hee (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Choi, Moon-Kyoung (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Kim, Min-Ji (Department of Food Science and Technology / Institute of Food Science, Pukyong National University) ;
  • Kim, Tae-Wan (Department of Food Science & Biotechnology, Andong National University) ;
  • Ahn, Dong-Hyun (Department of Food Science and Technology / Institute of Food Science, Pukyong National University)
  • 이소정 (부경대학교 식품공학과 / 식품연구소) ;
  • 송유진 (부경대학교 식품공학과 / 식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과 / 식품연구소) ;
  • 이청조 (부경대학교 식품공학과 / 식품연구소) ;
  • 정지연 (부경대학교 식품공학과 / 식품연구소) ;
  • 곽지희 (부경대학교 식품공학과 / 식품연구소) ;
  • 최문경 (부경대학교 식품공학과 / 식품연구소) ;
  • 김민지 (부경대학교 식품공학과 / 식품연구소) ;
  • 김태완 (안동대학교 식품생명공학과) ;
  • 안동현 (부경대학교 식품공학과 / 식품연구소)
  • Received : 2010.10.19
  • Accepted : 2010.12.03
  • Published : 2010.12.31
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Abstract

This study was performed to investigate the inhibitory activity of Sargassum thunbergii (ST) against ${\alpha}$-amylase and elucidate the availability of ST extract as a functional food agent. To test the inhibitory activity of ST against ${\alpha}$-amylase, porcine pancreatic ${\alpha}$-amylase and potato starch were used as substrates. It was revealed that ST crude ethanol extracts have high ${\alpha}$-amylase inhibitory activity. Subsequently, ST crude ethanol extract was separated into five partition layers by solvent extraction: n-hexane, chloroform, ethyl acetate, butanol, and water. Chloroform and n-hexane fractions showed higher inhibitory activities than did acarbose (positive control). To confirm the changes in enzyme inhibitory activity by physical treatments, ST crude ethanol extract was subjected to heat, pH, and ${\gamma}$-irradiation treatments. In all heat treatments with the exception of one ($121^{\circ}C$, 15 min), the inhibitory activity was increased compared with the untreated group. With regard to pH stability, ST extract showed no significant changes at pH 4.6, but somewhat decreased inhibitory activity was revealed at pH 2, 8, and 10. On the other hand, ST ethanol extract was stable under ${\gamma}$-irradiation under all conditions (3.20 kGy). In summary, ST ethanol extract can be used in the food industry as a natural ${\alpha}$-amylase inhibitor.

Keywords

References

  1. Ali H, Houghton PJ and Soumyanath A. 2006. $\alpha$-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 107, 449-455. https://doi.org/10.1016/j.jep.2006.04.004
  2. Baht M, Zinjarde SS, Bhargava SY, Kumar AR and Joshi BN. 2008. Antidiabetic Indian plats : a good source of potent amylase inhibitors. eCAM, 1-6.
  3. Bitou N, Ninomiya M, Tsujita T and Okuda H. 1999. Screening of lipase inhibitors from marine algae. Lipids 34, 441-445. https://doi.org/10.1007/s11745-999-0383-7
  4. Cho SH, Park SY and Choi SW. 2008. Effects of Eisenia bicyclis extracts and pill on blood glucose and lipid profile in streptozotocin-induced diabetic mice. Korean J Nutr 41, 493-501.
  5. Choi HJ. Kang JS, Choi YW, Jeong YK and Joo WH. 2008a. Inhibitory activity on the diabetes related enzymes of Tetragonia tetragonoides. Korean J Biotechnol Bioeng 23, 419-424.
  6. Choi HJ, Jeong YK, Kang DO and Joo WH. 2008b. Inhibitory effects of four solvent fractions of Alnus firma on α-amylase and $\alpha$-glucosidase. J Life Sci 18, 1005-1010. https://doi.org/10.5352/JLS.2008.18.7.1005
  7. Choi SY, Kim SY, Hur JM, Choi HG and Sung NJ. 2006a. Antioxidant activity of solvent extracts from Sargassum thunbergii. J Korean Soc Food Sci Nutr 35, 139-144. https://doi.org/10.3746/jkfn.2006.35.2.139
  8. Choi Y, Lee SM, Chun J, Lee HB and Lee J. 2006b. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of shiitake (Lentinus edodes) mushroom. Food Chem 99, 381-387. https://doi.org/10.1016/j.foodchem.2005.08.004
  9. Chrzaszcz T and Janicki J. 1933. "Sisto-amylase", a natural inhibitor of amylase. Chem Abstr 27, 3491-3505.
  10. Gao H and Kawabata J. 2005. $\alpha$-Glucosidase inhibition of 6-hydroxyflavone s. Part 3: synthesis and evaluation of 2,3,4-trihydroxybenzoyl-containing flavonoid analogs and $\sigma$-aminoflavones as $\alpha$-glucosidase inhibitors. Bioorg Med Chem 13, 1661-1671. https://doi.org/10.1016/j.bmc.2004.12.010
  11. Granum PE. 1978. Purification and characterization of an amylase inhibitor from rye (Secale cerale) flour. J Food Biochem 2, 103-120. https://doi.org/10.1111/j.1745-4514.1978.tb00607.x
  12. Ha JK, Emerick RJ and Embry LB. 1985. Effect of buffers and alfalfa hay on ruminal and systemic parameters of sheep fed high concentrate diets. Kor J Anim Sci 27, 80-96.
  13. Hara Y and Honda M. 1990. The inhibition of $\alpha$-amylase by tea polyphenols. Agric Biol Chem 54, 1939-1945. https://doi.org/10.1271/bbb1961.54.1939
  14. Heo SJ, Hwang JY, Choi JI, Han JS, Kim HJ and Jeon YJ. 2009. Diphlorethohydroxy carmalol isolated from Ishige okamurae, a brown algae, a potent $\alpha$-glucosidase and $\alpha$-amylase inhibitor, alleviates postprandial hyperglycemia in diabetic mice. Eur J Pharmacol 615, 252-256. https://doi.org/10.1016/j.ejphar.2009.05.017
  15. Iwai K. 2008. Antidiabetic and antioxidant effects of polyphenols in brown alga Ecklonia stolonifera in genetically diabetic KK-Ay mice. Plant Foods Hum Nutr 63, 163-169. https://doi.org/10.1007/s11130-008-0098-4
  16. Kageyama S, Nakamichi N, Sekino H and Nakano S. 1997. Comparison of the effects of acarbose and voglibose in healthy subjects. Clin Ther 19, 720-729. https://doi.org/10.1016/S0149-2918(97)80096-3
  17. Kang HJ, Jo C, Kim DJ, Seo JS and Byun MW. 2003. Physiological activities of citrus peel extracts by different extraction methods and gamma irradiation. Korean J Food Preserv 10, 388-393.
  18. Kim MS, Ahn SM, Jung IC, Kwon GS and Shon HY. 2010. Screening of $\alpha$-amylase and $\alpha$-glucosidase inhibitor from Nepalese plant extracts. Kor J Microbiol Biotechnol 2, 183-189.
  19. Kim HJ, Jo C, Kim TH, Kim DS, Park MY and Byun MW. 2006. Biological evaluation of the methanolic extract of Eriobotrya japonica and its irradiation effect. Korean J Food Sci Technol 38, 684-690.
  20. Kim HY. 1997. In vitro inhibitory activity on rat intestinal mucosa $\alpha$-glucosidase by rice hull extract. Korean J Food Sci Technol 29, 601-608.
  21. Kim JH, Kim MU and Cho YJ. 2007. Isolation and identification of inhibitory compound from Crataegi fructus on $\alpha$-amylase and $\alpha$-glucosidase. J Korean Soc Appl Biol Chem 50, 204-209.
  22. Kim KH, Roh SG, Li CR, Jin CF, Kim A and Choi WC. 2008. Anti-diabetic effects of banaba leaf extracts (Lagerstroemia speciosa Pers.) through solvents. JLS 18, 1305-1311. https://doi.org/10.5352/JLS.2008.18.9.1305
  23. Lim CS, Li CY, Kim YM, Lee WY and Rhee HI. 2005. The inhibitory effect of Cornus walteri extract against $\alpha$-amylase. J Korean Soc Appl Biol Chem 48, 103-108.
  24. Lee BB, Park SR, Han CS, Han DY, Park EJ, Park HR and Lee SC. 2008. Antioxidant activity and inhibitory activity against $\alpha$-amylase and $\alpha$-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 37, 405-409. https://doi.org/10.3746/jkfn.2008.37.4.405
  25. Lee SH, Li Y, Karadeniz F, Kim MM and Kim SK. 2009a. $\alpha$-Glucosidase and $\alpha$-amylase inhibitory activities of phloroglucinal derivatives from edible marine brown alga, Ecklonia cava. J Sci Food Agric 89, 1552-1558. https://doi.org/10.1002/jsfa.3623
  26. Lee SJ, Song EJ, Lee SY, Kim KBWR, Yoon SY, Lee CJ, Jung JY, Park NB, Kwak JH, Par JG, Kim JH, Choi JI, Lee JW, Byun MW and Ahn DH. 2010. Effects of gamma irradiation on antioxidant, antimicrobial activities and physical characteristics of Sargassum thunbergii extract. Korean J Fooc Sci Technol 42, 431-437.
  27. Lee SY, Song EJ, Kim KBWR, Yoon SY, Kim SJ, Lee SJ, Hong YK, Lim SM and Ahn DH. 2009b. Antimicrobial activity of ethanol extract from Sargassum thunbergii. J Korean Soc Food Sci Nutr 38, 502-508. https://doi.org/10.3746/jkfn.2009.38.4.502
  28. Mahmud T, Tornus I, Egelkrout E, Wolf E, Uy C, Floss HG and Lee SS. 1999. Biosynthetic studies on the $\alpha$-glucosidase inhibitor acarbose in Actinoplanes sp.; 2-epi-5-epi-valiolone is the direct precursor of the valienamine moiety. J American Chem Soc 121, 6973-6983. https://doi.org/10.1021/ja991102w
  29. Moon JS, Bae YI and Shim KH. 1998. The physicochemical properties of $\alpha$-amylase inhibitors from black bean and naked barley in Korea. J Korean Soc Food Sci Nutr 37, 367-375.
  30. Mulimani VH and Rudrappa G. 1994. Effect of heat treatment and germination on alpha amylase inhibitor activity in chick peas (Cicer arietinum L.). Plant Food Human Nutr 46, 133-137. https://doi.org/10.1007/BF01088765
  31. McLaughlin CL, Thompson A, Greenwood K, Sherington J and Bruce C. 2009. Effect of acarbose on acute acidosis. J Dairy Sci 92, 2758-2766. https://doi.org/10.3168/jds.2008-1602
  32. Puls W and Neup U. 1973. Influence of $\alpha$-amylase inhibitor (BAY D7791) on blood-glucose, seruminsulin and NEFA in starch loading test in rats, dogs and man. Diabetologia 9, 97-101. https://doi.org/10.1007/BF01230687
  33. Ryu BH, Kim DS, Cho K and Sin DB. 1989. Antitumor activity of seaweeds agar on Sarcoma-180. Korean J Food Sci Technol 21, 595-600.
  34. Swain T, Hillis WE. 1959. The phenolic constituents of Prunus domestica. I-The quantitative analysis of phenolic constituents. J. Sci. Food Agric., 10, 63-68. https://doi.org/10.1002/jsfa.2740100110
  35. Yang F, Wang L and Hu Q. 2005. Preparation of polysaccharide derived from Sargassum thunbergii and its antioxidant activity. Food Sci 26, 224-227.
  36. Yook HS, Cha BS, Jo SK and Byun MW. 1998. Effects of gamma irradiation on microbial decontamination, extraction yields and physiological effectiveness of Korean medicinal plants. Korean J Food Sci Technol 30, 581-589.

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