한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제43권6호
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  • Pages.637-641
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  • 2010
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  • 0374-8111(pISSN)
  • /
  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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해수에서 분리한 장염비브리오의 항생제 내성 및 암피실린 내성 유전자의 동정

Antibiotic-Resistance Profiles and the Identification of the Ampicillin-Resistance Gene of Vibrio parahaemolyticus Isolated from Seawater

  • 이근우 (군산대학교 식품생명공학과) ;
  • 박권삼 (군산대학교 식품생명공학과)
  • Lee, Kuen-Woo (Department of Food Science and Biotechnology, Kunsan National University) ;
  • Park, Kwon-Sam (Department of Food Science and Biotechnology, Kunsan National University)
  • 투고 : 2010.07.21
  • 심사 : 2010.12.06
  • 발행 : 2010.12.31
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초록

The antibiotics-resistance profiles of 28 strains of Vibrio parahaemolyticus isolated from seawater were investigated. All of the strains studied were resistant to ampicillin (100%), but susceptible to 12 other antibiotics. The minimum inhibitory concentration (MIC) of V. parahaemolyticus to ampicillin was as high as $1,024-2,048\;{\mu}g{\cdot}mL^{-1}$. The phenotype of strain 8 changed from ampicillin-resistant to susceptible with an in-frame deletion mutant of VPA0477, a putative ${\beta}$-lactamase gene, and the MIC for ampicillin of the mutant strain was $1{\mu}g{\cdot}mL^{-1}$. In conclusion, our findings suggest that the VPA0477 gene acts as a ${\beta}$-lactamase in ampicillin-resistant V. parahaemolyticus strains.

키워드

참고문헌

  1. Acar JF and Goldstein FW. 1991. Disk susceptibility test. In: Antibiotics in Laboratory Medicine, Lorian V, ed. Williams & Wilkins, Baltimore, U.S.A., 17-52.
  2. Baker-Austin C, McArthur JV, Tuckfield RC, Najarro M, Lindell AH, Gooch J and Stepanauskas R. 2008. Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carilina, USA. J Food Prot 71, 2552-2558. https://doi.org/10.4315/0362-028X-71.12.2552
  3. Ceccarelli D, Salvia AM, Sami J and Cappuccinelli P. 2006. New cluster pf plasmid-located class I integrons in Vibrio cholerae 01 and a dfrA15 cassettecontaining integron in Vibrio parahaemolyticus isolated in Angola. Antimicrob Agents Chemother 50, 2493-2499.
  4. Ferrini AM, Mannoni V, Suffredini E, Cozzi L and Croci L. 2008. Evaluation of antibacterial resistance in Vibrio strains isolated from imported seafood and ltalian aquaculture settings. Food Anal Methods 1, 164-170. https://doi.org/10.1007/s12161-007-9011-2
  5. Honda T and Iida T. 1993. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins. Rev Med Microbiol 4, 106-113. https://doi.org/10.1097/00013542-199304000-00006
  6. Kodama T, Hiyoshi H, Gotoh K, Akeda Y, Matsuda S, Park KS, Cantarelli VV, Iida T and Honda T. 2008. Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2. Infect Immun 76, 4282-4289. https://doi.org/10.1128/IAI.01738-07
  7. Kodama T, Rokuda M, Park KS, Cantarelli VV, Matsuda S, Iida T and Honda T. 2007. Identification and characterization of VopT, a novel ADPribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2. Cell Microbiol 9, 2598-2609. https://doi.org/10.1111/j.1462-5822.2007.00980.x
  8. Lee CY, Cheng MF, Yu MS and Pan MJ. 2002. Purification and characterization of a putative virulence factor, serine protease, from Vibrio parahaemolyticus. FEMS Microbiol Lett 19, 31-37.
  9. Lee H, Oh YH, Park SG and Choi SM. 2007. Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. Kor J Env Hlth 33, 16-20. https://doi.org/10.5668/JEHS.2007.33.1.016
  10. Lee HW, Lim SK and Kim MN. 2009. Characteristics of ampicillin-resistant Vibrio spp. isolated from a west coastal area of Korea peninsula. J Kor Fish Soc 42, 20-25.
  11. Makino K, Oshima K, Kurokawa K, et al., 2003. Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae. Lancet 361, 743-749. https://doi.org/10.1016/S0140-6736(03)12659-1
  12. Miller VL and Mekalanos JJ. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxr. J Bacteriol 170, 2575-2583. https://doi.org/10.1128/jb.170.6.2575-2583.1988
  13. NCCLS. 2004. Performance standards for antimicrobial susceptibility testing. Twelfth Information Supplement. NCCLS. 2000; Document. M100-S12 (ISBN, 1-56238-456-4) NCCLS, 940 West Valley Road, Suite, 1400, Wayne, PA:19087-19098. U.S.A.
  14. Oh EG, Yu HS, Shin SB, Son KT, Park KB, Kwon JY, Lee TS and Lee HJ. 2008. Trimethoprim resistance of Vibrio parahaemolyticus isolated from the fish farm. J Kor Fish Soc 41, 324-329. https://doi.org/10.5657/kfas.2008.41.5.324
  15. Park KS, Arita M, Iida T and Honda T. 2005. vpaH, a gene encoding a novel histone-like nucleoid structure-like protein that was possibly horizontally acquired, regulates the biogenesis of lateral flagella in trh-positive Vibrio parahaemolyticus TH3996. Infect Immun 73, 5754-5761. https://doi.org/10.1128/IAI.73.9.5754-5761.2005
  16. Park KS, Ono T, Rokuda M, Jang MH, Okada K, Iida T and Honda T. 2004. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect Immun 72, 6659-6665. https://doi.org/10.1128/IAI.72.11.6659-6665.2004
  17. Rowe-Magnus DA and Mazel D. 2002. The role of integrons in antibiotic resistance gene capture. Int J Med Microbiol 292, 115-125. https://doi.org/10.1078/1438-4221-00197
  18. Sakazaki R, Tamura K, Kato T, Obara Y and Yamai S. 1968. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. 3. Enteropathogenicity. Jpn J Med Sci Biol 21, 325-331. https://doi.org/10.7883/yoken1952.21.325
  19. Son JC, Park SW and Min KJ. 2003. Environmental and antimicrobial characteristics of Vibrio spp. isolated from fish, shellfish and brackish water samples in Gyeongbuk eastern coast. Kor J Env Hlth 29, 94-102.
  20. Son KT, Oh EG, Lee TS, Lee HJ, Kim PH and Kim JH. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from farms on the southern coast of Korea. J Kor Fish Soc 38, 365-371. https://doi.org/10.5657/kfas.2005.38.6.365
  21. Tanil GB, Radu S, Nishibuchi M, Rahim RA, Napis S, Maurice L and Gunsalam JW. 2005. Characterization of Vibrio parahaemolyticus isolated from coastal seawater in peninsular Malaysia. Southeast Asian J Trop Public Health 36, 940-945.
  22. Yu HS, Park KB, Oh EG, Lee TS, Shin SB, Kwon JY, Kim JH and Son KT. 2010. Trimethoprim resistance by class I integron in Vibrio parahaemolyticus from a fish farm. Kor J Fish Aquat Sci 43, 125-130. https://doi.org/10.5657/kfas.2010.43.2.125

피인용 문헌

  1. Antimicrobial-resistance Profiles and Virulence Genes of Vibrio parahaemolyticus Isolated from Seawater in the Wando Area vol.47, pp.3, 2014, https://doi.org/10.5657/KFAS.2014.0220
  2. Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction vol.47, pp.6, 2014, https://doi.org/10.5657/KFAS.2014.0740
  3. Antibiotic Susceptibility of Vibrio spp. Isolated from West Sea vol.49, pp.2, 2013, https://doi.org/10.7845/kjm.2013.3028
  4. Antibiotic Resistance and Plasmid Profile of Vibrio parahaemolyticus Strains Isolated from Kyunggi-Incheon Coastal Area vol.48, pp.1, 2012, https://doi.org/10.7845/kjm.2012.48.1.022
  5. 남해안 패류양식장에서 분리한 Vibrio parahaemolyticus의 병원인자 분포 및 항균제 내성 vol.49, pp.4, 2010, https://doi.org/10.5657/kfas.2016.0460