Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Synergistic Effects of 5-Fluorouracil (FU) and Curcumin on Human Cervical Cancer Cells

5-fluorouracil과 curcumin의 복합투여에 의한 자궁암세포의 성장억제와 p53유전자 발현의 상승 효과

  • Ahn, Seong-Ho (Department of Genetic Engineering, Cheongju University) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University) ;
  • Kang, Jung-Hoon (Department of Genetic Engineering, Cheongju University) ;
  • Lee, Myeong-Seon (Department of Genetic Engineering, Cheongju University)
  • 안성호 (청주대학교 이공대학 유전공학과) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 강정훈 (청주대학교 이공대학 유전공학과) ;
  • 이명선 (청주대학교 이공대학 유전공학과)
  • Received : 2010.11.17
  • Accepted : 2010.12.26
  • Published : 2010.12.31
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Abstract

Cervical cancer is associated with low antioxidant status. It has a high prevalence especially amongst woman in Asia and is a leading cause of cancer death. Cancer chemotherapy in vivo improved in cases with high p53 expression in the tumor tissue. The restoration of p53 levels could be a potential strategy to increase chemoresponsiveness. Under circumstances where damage is extensive, p53 plays a direct role in trigering apoptosis. To investigate the effect of curcumin (CMN) as an antioxidant agent on anticancer agent 5-fluorouracil (5FU) induced apoptosis and p53 expression, HPV-18 positive HeLa cells were treated with noncytotoxic amounts of antioxidant. Curcumin induced apoptosis in cervical cancer cells. Morphological hallmarks of apoptosis such as nuclear fragmentation and internucleosomal fragmentation of DNA were observed. CMN caused upregulation of p53 expression, evident from Western blotting data and also increased the susceptibility/apoptosis induced by 5FU. These results show that increasing drug sensitivity of cervical cancer cells by upregulation of p53 using CMN is novel approach and could have a possible therapeutic potential in cervical cancer.

암은 전 세계적으로 사망률이 높은 질병으로 현재 자궁암 치료는 cisplatin, 5-fluorouracil (5FU) 등의 항암화학요법과 방사선 요법 등을 복합적으로 병행하고 있다. 최근 항암효과를 가지고 있는 자연식품의 섭취에 관심이 커지면서 커리의 주성분인 curcumin (CMN), 녹차의 주성분인 카테킨, 토마토의 주성분인 리코펜 등의 약리효과에 대한 연구가 활성화되고 있다. CMN은 동물실험 결과 항염증의 활성이 있고, 피부, 대장, 유방, 전립선 등에서 암으로의 진행을 억제한다는 보고가 있으나 인체 자궁암에서는 그 효과가 밝혀져 있지않다. 본 연구는 항암제 5FU와 CMN이 인체 자궁암세포 HeLa에 미치는 영향을 apoptosis의 유도로 평가하고, p53유전자 발현율과의 상관관계로 확인하고자 시행하였다. CMN은 HeLa 세포의 성장을 억제하였으며, 5FU로 유도된 apoptosis의 발생률과 p53유전자의 발현률을 현저하게 증가시키는 것으로 나타났다(p<0.05). 이러한 연구결과는 CMN이 자궁암 치료제의 가능성이 있으며, 또한 5FU와 복합적으로 사용하면 항암제를 단독으로 투여하는 경우보다 자궁암 치료에 보다 효과적임을 강력히 시사하는 것이다.

Keywords

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