Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

The Immunoadjuvant Activity of The Water-Extract of Korean mistletoe (Viscum album var. coloratum) Fruit

한국산 겨우살이 열매 추출물의 Immunoadjuvant 효과

  • Lee, Jung-Lim (Department of Microbiology, College of medicine, Konyang University) ;
  • Ahn, Jae-Hyung (Department of Microbiology, College of medicine, Konyang University) ;
  • Hwang, Seong-Gu (Division of Animal Life and Environmental Science, Hankyong National University) ;
  • Jung, Yeon-Hwa (Department of Microbiology, College of medicine, Konyang University) ;
  • Yang, Hyo-Seon (Department of Microbiology, College of medicine, Konyang University) ;
  • Kang, Tae-Bong (Department of Biotechnology & Food Science, Handong University) ;
  • Kim, Jong-Bae (Department of Biotechnology & Food Science, Handong University) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of medicine, Konyang University)
  • 이정림 (건양대학교 의과대학 미생물학교실) ;
  • 안재형 (건양대학교 의과대학 미생물학교실) ;
  • 황성구 (한경대학교 동물생명환경과학부) ;
  • 정연화 (건양대학교 의과대학 미생물학교실) ;
  • 양효선 (건양대학교 의과대학 미생물학교실) ;
  • 강태봉 (한동대학교 생명식품공학부) ;
  • 김종배 (한동대학교 생명식품공학부) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실)
  • Received : 2010.09.02
  • Accepted : 2010.10.20
  • Published : 2011.12.31
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Abstract

To evaluate the immunomodulatory activity of a water extract (KMF-WE) of Korean mistletoe (Viscum album var. coloratum) fruit, we examined its ability to induce humoral and cellular immune response against keyhole limpet hemocyanine (KLH). Immunized mice with KLH admixed with KMF-WE (KLH/KMF-WE) showed significant induction of KLH-specific antibodies compared to mice immunized with KLH alone. The assay for determining isotypes of antibodies revealed that KMFWE augmented KLH-specific-IgG1 and -IgG2a production. In vitro T lymphocyte proliferation analysis against KLH revealed that the splenocytes of mice immunized with KLH/KMF-WE showed a significantly higher proliferative ability than those from mice immunized with KLH alone. The culture supernatants of splenocytes, which were harvested from mice immunized with KLH/KMF-WE, showed higher levels of both Th-1 type (IL-2, IFN-${\gamma}$) and Th-2 type (IL-4) cytokines in response to KLH stimulation compared to those from mice immunized with KLH alone. Also, in delayed-type hypersensitivity (DTH) assay, mice immunized with KLH/KMF-WE showed a significantly higher reaction to KLH than mice treated with KLH alone. These results suggest that KMF-WE possess immunoadjuvant activity to enhance both antigen-specific humoral and cellular immune responses against protein antigens (KLH).

Keywords

References

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