Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Immobilization of β-Glucosidase from Exiguobacterium sp. DAU5 on Chitosan Bead for Improved Enzymatic Properties

효소 특성 개선을 위한 Exiguobacterium sp. β-glucosidase의 키토산 비드에 효소 고정화

  • Received : 2010.09.23
  • Accepted : 2010.10.28
  • Published : 2010.11.30
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Abstract

Glutaraldehyde was used to cross-link chitosan beads to immobilize the crude enzyme $\beta$-glucosidase from Exiguobacterium sp. DAU5. The conditions for preparing cross-linking chitosan beads and immobilization such as concentration of glutaradehyde, cross-linking time, immobilization pH and time were optimized. The chitosan beads were cross-linked with 1.5% glutaraldehyde for 1.5 hr. The immobilized $\beta$-glucosidase had an overall yield of 20% and specific activity of 5.22 U/g. The optimized pH and temperature were 9.0 and $55^{\circ}C$, respectively. More than 80% of its activity at pH 7.0-10.0, 80% at $40^{\circ}C$ for 2 hr and 48% at $50^{\circ}C$ for 1 hr, were retained. However, the immobilization product showed higher pH and thermal stabilities than free enzymes. It also showed high hydrolyzing activity on soybean isoflavone glycoside linkage. These results suggest the broad application prospects of immobilization enzymes.

Exiguobacterium sp. 유래의 $\beta$-glucosidase 고정을 위하여 글루타르알데하이드를 사용한 키토산 비드를 조제하였다. 키토산 비드의 교차결합 및 고정화의 조건을 최적화하였다. $\beta$-glucosidase 고정화의 최적생산 조건에서 20%의 수율과 5.22 U/g의 효소활성을 나타냈다. 최적 pH 와 온도는 9.0과 $55^{\circ}C$를 나타냈다. 고정된 효소의 안정성은 pH 7.0-10.0에서는 80%, $40^{\circ}C$ 2시간 반응에서는 80% 및 $50^{\circ}C$ 1시간 반응에서는 48%의 활성을 보유하였다. 이러한 결과는 높은 pH와 고온에서 비고정 효소보다 안정성을 보여주었다. 고정된 효소를 가지고 대두 이소플라본 배당체의 높은 가수분해능을 확인하였다. 이상의 결과는 고정화 효소의 다양한 이용 가능성을 시사하였다.

Keywords

References

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