Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Assessment of free-radical-scavenging and antibacterial activities, and brine shrimp toxicity of Scutellaria pinnatifida (Lamiaceae)

  • Sauvage, Severine (School of Biomedical Sciences, University of Ulster) ;
  • Samson, Emilie (School of Biomedical Sciences, University of Ulster) ;
  • Granger, Melanie (School of Biomedical Sciences, University of Ulster) ;
  • Majumdar, Anisha (School of Biomedical Sciences, University of Ulster) ;
  • Nigam, Poonam (School of Biomedical Sciences, University of Ulster) ;
  • Nahar, Lutfun (Drug Discovery and Design Research Division, Department of Pharmacy, School of Applied Sciences, University of Wolverhampton) ;
  • Celik, Sezgin (Central Laboratories, Kyrykkale University) ;
  • Sarker, Satyajit D. (Department of Pharmacy, School of Applied Sciences, University of Wolverhampton)
  • Received : 2010.03.22
  • Accepted : 2010.12.06
  • Published : 2010.12.31
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Abstract

Scutellaria pinnatifida A. Hamilt. (Lamiaceae) is an endemic Turkish herb. This plant is also endemic to Iran, and grows abundantly in other central and western Asian countries. Several species of the Scutellaria are known for their traditional uses in the treatment of hypertension, arteriosclerosis, inflammatory diseases, hepatitis, allergy, cancer and diarrhoea. Free-radical-scavenging property, antibacterial activity and brine shrimp toxicity of the n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of S. pinnatifida were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtitre plate based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts exhibited free-radical-scavenging property, with the $RC_{50}$ values of 0.362 and 0.127 mg/ml, respectively. Among the solid-phase extraction fractions of the MeOH extract, the 50% aqueous-MeOH fraction showed the highest level of free-radicalscavenging activity ($RC_{50}$ = 0.039 mg/ml). While the DCM extract showed low level of antibacterial activity against Bacillus subtilis and ampicillin-resistant Escherichia coli, the MeOH extract was active against B. cereus, B. subtilis, E. coli and ampicillin-resistant E. coli. However, the minimum inhibitory concentrations (MIC) of the MeOH extract against these bacterial strains were >10 mg/ml. None of the extracts showed any significant toxicity towards brine shrimps ($LD_{50}$ = > 1.00 mg/ml).

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References

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