Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Complexation of Adiponectin-encoding Plasmid DNA with Rosiglitazone-loaded Cationic Liposomes

  • Davaa, Enkhzaya (Department of Physical Pharmacy, College of Pharmacy, Chungnam National University) ;
  • Jeong, Ui-Hyeon (Department of Physical Pharmacy, College of Pharmacy, Chungnam National University) ;
  • Shin, Baek-Ki (Department of Physical Pharmacy, College of Pharmacy, Chungnam National University) ;
  • Choi, Soon-Gil (Department of Physical Pharmacy, College of Pharmacy, Chungnam National University) ;
  • Myung, Chang-Seon (Department of Pharmacology, College of Pharmacy, Chungnam National University) ;
  • Park, Jeong-Sook (Department of Physical Pharmacy, College of Pharmacy, Chungnam National University)
  • Received : 2010.10.27
  • Accepted : 2010.11.27
  • Published : 2010.12.20
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Abstract

To enhance therapeutic effects of insulin-sensitizing adipokine, ADN gene and potent agonists, rosiglitazone for the $PPAR{\gamma}$, cationic liposomes as non-viral vectors were formulated. The particle size and zeta potential of drug loaded and unloaded cationic liposomes were investigated. The complex formation between cationic liposomes and negatively charged plasmid DNA was confirmed and the protection from DNase was observed. In vitro transfection was investigated in HepG2, HeLa, and HEK293 cells by mRNA expression of ADN. Encapsulation efficacy of rosiglitazone-loaded liposomes was determined by UV detection. Particle sizes of cationic liposomes were in the range of 110-170 nm and those of rosiglitazone-loaded cationic liposomes were in the range of 130-180 nm, respectively. Gel retardation of complexes indicated that the complex was formed at weight ratios of cationic lipid to plasmid DNA higher than 20:1. Both complexes protected plasmid DNA from DNase either drug free or drug loading. Encapsulation efficiency of rosiglitazone-loaded emulsion was increased by drug dose. The mRNA expression levels of ADN were dose-dependently increased in cells transfected with plasmid DNA. Therefore, cationic liposomes could be potential co-delivery system for drug and gene.

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References

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