Korean Journal of Agricultural and Forest Meteorology (한국농림기상학회지)

Korean Society of Agricultural and Forest Meteorology (한국농림기상학회)
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Suitability of Physiological Indicators of Ozone Tolerance among 8 families of Sophora japonica

회화나무 8 가계간 오존 내성 차이에 대한 생리적 지표의 적합성

  • Han, Sim-Hee (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Kim, Du-Hyun (Department of Forest Resources Development, Korea Forest Research Institute) ;
  • Lee, Jae-Cheon (Department of Forest Resources Development, Korea Forest Research Institute)
  • 한심희 (국립산림과학원 산림자원육성부) ;
  • 김두현 (국립산림과학원 산림자원육성부) ;
  • 이재천 (국립산림과학원 산림자원육성부)
  • Received : 2010.07.27
  • Accepted : 2010.09.01
  • Published : 2010.09.30
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Abstract

This study was conducted to investigate ozone sensitivity of physiological indicators and the difference in ozone tolerance of 8 families of Sophora japonica seedlings on the basis of the standardized physiological indicators. After ozone treatment, photosynthetic parameters, photosynthetic pigments and malondialdehyde (MDA) content, and antioxidative enzyme activities were analyzed from the leaves of S. japonica seedlings. Ozone tolerance indices among 8 families were calculated with the standardized physiological parameters. In addition, the reduction of carboxylation efficiency and apparent quantum yield were observed in the leaves of seven families, except for family No. 6 and 7, respectively. The apparent quantum yield varied from -27% to -61% of the control seedlings. Photosynthetic pigment content differed significantly among 8 families, but was not affected significantly by ozone treatment. Superoxide dismutase (SOD) activity increased from 7% to 64% after ozone exposure, and significant difference existed among 8 families. Ascorbate-peroxidase (APX) activity of 8 families increased by ozone treatment, and the activity of family No. 7 showed the highest increase (218%) in comparison to their respective control plants. On the basis of the standardized indices, family No. 6 showed the lowest tolerance by indicating higher reduction of both photosynthetic parameters and pigment content and lower increase of antioxidative enzyme activities. On the contrary, family No. 7 showed the highest tolerance as indicated by lower reduction of photosynthetic parameters, higher amounts of photosynthetic pigments, and higher enzyme activity.

본 연구는 오존에 노출된 회화나무 8 가계에서 측정한 생리적 지표들을 대상으로, 오존에 대한 민감성을 평가하고, 이 결과들을 종합하여 가계 간 차이를 구명하고자 실시하였다. 회화나무(Sophora japonica L.) 8가계를 대상으로 오존 처리 후, 광합성 특성, 엽록소 함량, 항산화효소 활성 및 MDA 함량을 측정하였고, 각 측정 지표들의 표준화 지수를 이용하여 내성 순위를 결정하였다. 탄소고정효율은 6번 가계를 제외한 모든 가계에서 오존 처리 후 뚜렷하게 감소하였으며, 탄소고정효율의 감소율은 -38%에서 -67% 범위를 보였다. 또한 순양자수율은 7번 가계를 제외한 모든 가계에서 오존 처리 후 뚜렷한 감소를 보였으며, 감소율은 -27%에서 -61%를 나타냈다. 엽록소 a를 비롯한 광색소 함량의 가계 간 차이는 컸으나, 오존 처리 효과는 나타나지 않았다. 오존 처리 후, SOD 활성의 증가율은 7%에서 64%로, 가계 간 차이가 뚜렷하였다. APX 활성은 모든 가계에서 오존 처리 후 증가하였으며, 7번 가계가 가장 큰 활성 증가율(218%)을 보였다. 표준화 지수를 기준으로 할 때, 6번 가계의 내성 수준이 가장 낮았으며, 7번 가계의 내성 수준이 가장 높았다. 6번 가계는 오존 처리 후 탄소고정효율을 비롯한 광합성 특성의 감소율과 광색소 함량의 감소율이 높게 나타난 반면, 내성을 나타내는 항산화효소의 활성 증가가 매우 적었다. 반대로 내성 수준이 높은 7번 가계는 오존 처리 후 광합성 특성의 감소율이 다른 가계에 비해 적었으며, 광색소 함량은 오히려 오존처리 후 증가하였고, 항산화효소의 증가율도 다른 가계에 비해 높게 나타났다.

Keywords

References

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