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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell line C3H/10T1/2 by gene trap screening

  • Wang, Mingke (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University) ;
  • Sun, Huiqin (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University) ;
  • Jiang, Fan (Department of Chemical Defense and Toxicology, College of Preventive Medicine, Third Military Medical University) ;
  • Han, Jing (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University) ;
  • Ye, Feng (Department of Chemical Defense and Toxicology, College of Preventive Medicine, Third Military Medical University) ;
  • Wang, Tao (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University) ;
  • Su, Yongping (Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University) ;
  • Zou, Zhongmin (Department of Chemical Defense and Toxicology, College of Preventive Medicine, Third Military Medical University)
  • Received : 2010.10.20
  • Accepted : 2010.10.28
  • Published : 2010.12.31
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Abstract

A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-${\beta}1$ and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance.

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References

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