KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Effect of Complex Extracts of Mushroom and Sharp Toothed Eel on the Skin Conditions with Atopic Dermatitis and Acne Symptoms

갯장어 및 버섯 혼합추출물의 아토피 및 여드름 피부개선 효과

  • Cha, Wol-Suk (Department of Biochemical Engineering, Chosun University) ;
  • Yoo, Ji-Hyun (Department of Biochemical Engineering, Chosun University) ;
  • Min, Myung-Ja (Department of Biochemical Engineering, Chosun University) ;
  • Nam, Hyung-Gun (Department of Biochemical Engineering, Chosun University) ;
  • Kim, Yun-Soo (Department of Biochemical Engineering, Chosun University) ;
  • Shin, Hyun-Jae (Department of Biochemical Engineering, Chosun University) ;
  • Kim, Jong-Soo (Division of Chemical Engineering, Chonbuk National University) ;
  • Choi, On-You (Department of Environmental health, Cho-dang University) ;
  • Kim, Ran (Department of Cosmetology, Wonkwang Health Science University) ;
  • Choi, Du-Bok (Biotechnology Lab, BK Company R&D center)
  • 차월석 (조선대학교 생명화학공학과) ;
  • 유지현 (조선대학교 생명화학공학과) ;
  • 민명자 (조선대학교 생명화학공학과) ;
  • 남형근 (조선대학교 생명화학공학과) ;
  • 김윤수 (조선대학교 생명화학공학과) ;
  • 신현재 (조선대학교 생명화학공학과) ;
  • 김종수 (전북대학교 화학공학부) ;
  • 최온유 (초당대학교 환경보건학과) ;
  • 김란 (원광보건대학 미용피부관리학과) ;
  • 최두복
  • Received : 2010.04.13
  • Accepted : 2010.06.01
  • Published : 2010.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate effect of complex extract of various mushrooms and sharp toothed eel on the skin conditions with atopic dermatitis and acne symptoms. The total phenol concentration was increased in order of hot water> ethanol> ethyl acetate> petroleum ether> chloroform extract. Especially, when the hot water extract was used, it was about 2-3 fold higher than that of ethyl acetate, petroleumether, and chloroform extract. When the complex extract concentration was increased from 1.0 to 50 mg/L, the DPPH scavenging rate increased from 10.1 to 81.4%. The reduction power was sharply increased from 0.05 to 0.27 (700 nm) when the complex extract concentration was increased from 25 to 75 mg/L. However, above 100 mg/L, it was not decreased. In the case of SOD-like activity, it was 45.7% at 100 mg/L. Total numbers of patients with atopic dermatitis were 15 and 5 patients with severe acne symptoms. According to photos taken before and after the treatment and questionnaire results, considerable improvements in skin conditions are observed in the patients with atopic dermatitis and acne. For atopic patients, erythema and edema have been improved but the degree of effect was dependent on the individual's constitution. Concerning acne, the effect of coating of the extracts was prominent for first week and the degree decreased with time till 4 weeks. The complex extracts wereefficient in soothing rash and maturation. Side effects such as a scar were not detected during the application and treatment.

본 연구에서는 갯장어 및 버섯 혼합추출물을 이용하여 아토피 피부염 및 여드름의 개선효과를 확인하기 위하여 항산화 테스트와 아토피 피부염 임상시험자 15명에게 4주동안 혼합추출물을 복용과 함께 환부에 도포하도록 하였다. DPPH 소거능은 혼합추출물 농도증가와 함께 증가하였다. 특히 혼합추출물농도가 1.0 mg/L에서 50 mg/L로 증가 할 경우 DPPH 소거능은 10.1에서 81.4%로 증가 하였다. 환원력도 추출물농도가 증가 함에 따라 증가 하였다. 특히 추출물 25 mg/L에서 75 mg/L로 증가 할 경우 0.05 OD (700 nm)에서 0.27 (700 nm)로 급격히 증가 하였고 추출물 농도가 100 mg/L일 경우는 0.31 (700 nm)였다. SOD활성은 추출물 농도가 100 mg/L일 경우는 45.7%였다. 아토피성 피부염의 가려움과 붉은 반점에 대하여 임상시험 결과 임상시험자 모두 완치에 가깝도록 개선되는 효과를 나타내었다. 여드름 피부의 임상시험자 5명 모두 임상시험 후 염증 면포가 완화되어 여드름의 등급이 낮아지는 결과를 나타내었다. 여드름 피부의 임상시험자 모두 얼굴피부의 유수분 및 거칠기 상태가 정상피부의 수준에 이르도록 긍정적인 변화를 나타내었다. 혼합추출물을 피부에 직접 적용하여 임상시험한 결과, 아토피 피부염 및 여드름 피부의 임상시험자 모두 어떠한 부작용도 나타내지 않았다. 이상의 결과는 갯장어 및 버섯 혼합추출물이 아토피 피부염 및 여드름 피부관리를 위한 의약품 및 화장품의 원료로 개발될 충분한 가치가 있는 것으로 사료된다.

Keywords

References

  1. Larsson, P. A. and S. Liden (1980) Prevalence of skin diseases among adolescents 12-16 years of age. Acta Derm. Vener. 415-423.
  2. Hanifin, J. M. (2004) Atopic dermatitis: broadening the perspective. J. Am. Acad. Dermatol. 51: 23-243.
  3. Jung, W. R., K. S. Moon, D. Y. Kong, and B. Y. Pyun (2005) An investigation into the actual condition of outbreak and treatment in atopic dermatitis. Pediatric Allergy Respiratory Disease. 15: 44-52.
  4. Kim, C. J. (1988) Pathophysiology. pp. 87-98. SinIl Press Inc., Korea.
  5. Kim, J. D. (1995) A study of the treatment and prevention of acne and skin care. Kor. Soci. Cosmet. 1: 101-118.
  6. Kim, G. H. (1995) Treatment of atopic dermatitis. Allergy. 15: 10-20.
  7. Kim, H. Y. (2000) Skin science. pp. 65-73. Sumun Press Inc., Korea.
  8. Cho, M. Y., J. H. Cho, J. H. Kang, I. O. Kang, and S. S. Bae (2205) Cosmetics. pp. 49-52. Humin Press. Inc., Korea.
  9. Kaur, C. and Kapoor, H. C. (2002) Anti-oxidant activity and total phenolic content of some asian vegetables. Inter. J. Food Sci. Technol. 37: 153-161. https://doi.org/10.1046/j.1365-2621.2002.00552.x
  10. Sulc, M., J. Lachman, K. Hamouz, and K. P. Dvora (2008) Impact of phenolic content on antioxidant activity in yellow and purple-fleshed potatoes grown in the Czech Republic. Bio. Agri. Horti. 26: 45-54. https://doi.org/10.1080/01448765.2008.9755068
  11. Fu, H. Y., D. E. Shieh, and C. T. Ho (2002). Antioxidant and free radical scavenging activities of edible mushrooms. J. Food Lipids. 9: 35-46. https://doi.org/10.1111/j.1745-4522.2002.tb00206.x
  12. Okuda, T., Y. Kimura, T. Yoshida, T. Hatano, H. Okuda, and S. Arichi (1983) Studies on the activity and related compounds from medicinal plants and drugs. Chem. Pharma. Bull. 31: 1625-1631. https://doi.org/10.1248/cpb.31.1625
  13. Beckman, J. S. and W. H. Koppenol (1996) Nitroxide, superoxide, and peroxynitrite: the good, the bad, and ugly. Am. J. Physiol. 217: 1421-1437.
  14. Tricker, A. R. and R. Preussmann (1991) Carcinogenic N-nitrosamines in the diet: occurrence, formation, mechanisms and carcinogenic potential. Mutat. Res. Genet. Toxicol. 259: 277-289. https://doi.org/10.1016/0165-1218(91)90123-4
  15. Kim, H. O., C. W. Park, C. H. Lee, and J. O. Lee (2005) Efficacy of 0.1% tacrolimus ointment in korean patients with atopic dermatitis. Kor. Skin sci. 14: 312-318.