Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Role of p53-dependent PI3K in Radioresistance of Colon Cancer Cells

대장암 세포의 방사선저항성에 대한 p53의존성 PI3K의 역할

  • Lee, Heui-Kwan (Department of Radiation Oncology, Presbyterian Medical Center) ;
  • Kim, Jong-Suk (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Kwon, Hyoung-Cheol (Department of Radiation Oncology & Research Institute of Clinical Medicine, Chonbuk National University Hospital)
  • 이희관 (예수병원 방사선 종양학과) ;
  • 김종석 (전북대학교 의학전문대학원 생화학 교실) ;
  • 권형철 (전북대학교병원 방사선 종양학과 및 임상의학연구소)
  • Received : 2010.03.09
  • Accepted : 2010.09.09
  • Published : 2010.09.30
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Abstract

Radiotherapy is one of the major therapies for cancer treatment. p53 acts as a central mediator of the cellular response to stressful stimuli, such as radiation. Recently it has been known that activation of the phosphatidylinositol-3-kinase (PI3K) pathway is associated with radioresistance. In this study, we investigated whether X-irradiation up-regulates PI3K in a p53-dependent manner in human colon cancer cells. In order to study this phenomenon, we have treated p53-wild type and p53-mutant type HCT116 cells with X-ray. Treatment of wild type HCT116 cells with 8 Gy resulted in a marked increase in PI3K (p85), which paralleled an increase in PTEN, a counterpart of PI3K. However, these effects of X-rays in the p53-mutant cells were not observed. These results suggest that the X-irradiation-induced up-regulation of PI3K/PTEN pathway is p53-dependent.

Keywords

References

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