The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

The Neuroprotective Effect of Acupuncture Treatment at Shaofu (HT8) on Kainic Acid-induced Epilepsy Mouse Model.

Kainic acid 유발 간질 생쥐모델에서 소부혈(少府穴) 침치료의 해마 신경세포 보호효과연구

  • Kim, Yoon-Young (Department of Pediatrics, Dongguk Univ-Seoul, Graduate School of Oriental Medicine) ;
  • Min, Sang-Yeon (Department of Pediatrics, Dongguk Univ-Seoul, Graduate School of Oriental Medicine) ;
  • Kim, Ji-Yong (Seocho Medical Clinic, Kangbuk Samsung Hospital) ;
  • Kim, Jang-Hyun (Department of Pediatrics, Dongguk Univ-Seoul, Graduate School of Oriental Medicine)
  • 김윤영 (동국대학교 한의과대학 소아과학교실) ;
  • 민상연 (동국대학교 한의과대학 소아과학교실) ;
  • 김지용 (강북삼성병원 서초부속의원) ;
  • 김장현 (동국대학교 한의과대학 소아과학교실)
  • Received : 2010.08.04
  • Accepted : 2010.09.06
  • Published : 2010.09.30
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Abstract

Objectives: The present study investigated the effects of acupuncture treatment and their mechanism by using the kainic acid (KA)-induced epilepsy mouse model. Materials and Methods: The seizure was induced by an intraperitoneal (i.p.) injection of 30 mg/kg KA, and the acupuncture treatment was subsequently administered to acupoint Shaofu(HT8) bilaterally with two pretreatment sessions before injection (total 3 times over 3 days). Twenty four hours after injection, we observed the survival of neuronal cells in the CA3 region of the hippocampus. In addition, the activation of microglia and astrocytes was observed by using CD11b and GFAP immunohistochemistry in the same region. Results: The results indicate that acupuncture treatment reduced the rate of neural cell death in the CA3 region of the hippocampus and decreased the activations of microglia and astrocytes in this region. Conclusion: These results demonstrate that acupuncture treatment protects hippocampal neuronal cell death from KA-induced epileptic seizure by inhibiting the activations of microglia and astrocytes.

Keywords

References

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