The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

The Hepatoprotective Activity of Spatholobi Caulis Water Extract against Cadmium-Induced Toxicity in Rats

계혈등 물추출물의 간세포 보호효과

  • Park, Won-Mook (Department of Oriental Internal Medicine, Daegu Haany University) ;
  • Choi, Hong-Sik (Department of Oriental Internal Medicine, Daegu Haany University) ;
  • Kim, Seung-Mo (Department of Oriental Internal Medicine, Daegu Haany University) ;
  • Woo, Chang-Hoon (Department of Oriental Rehabilitation Medicine, Daegu Haany University)
  • 박원묵 (대구한의대학교 한의학과 간계내과학교실) ;
  • 최홍식 (대구한의대학교 한의학과 간계내과학교실) ;
  • 김승모 (대구한의대학교 한의학과 간계내과학교실) ;
  • 우창훈 (대구한의대학교 한의학과 한방재활의학과교실)
  • Received : 2010.06.21
  • Accepted : 2010.09.09
  • Published : 2010.09.30
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Abstract

Objectives: This study was investigated the protective effect of Spatholobi Caulis water extract against cadmium (CdCl2, Cd)-induced hepatic toxicity in rats. Methods: To induce acute hepatic toxicity, Cd (4 mg/kg body weight) was dissolved in normal saline and intravenously injected into rats. Then, the rats received either a vehicle or silymarin (100 mg/kg) or Spatholobi Caulis water extract (30, 50 mg/kg/day) for 3 days, and were exposed to a single injection of Cd 24 h after the last Spatholobi Caulis/vehicle treatment. Results: Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were significantly increased by Cd treatment. In contrast, pretreatment with Spatholobi Caulis reduced ALT, AST and LDH. Cd-intoxicated liver damage was significantly inhibited by treatment of Spatholobi Caulis 30 and 50 mg/kg at histopathological observations in the present study. Conclusions: These results can be considered as direct evidence that Spatholobi Caulis has favorable inhibitory effects on the Cd-intoxicated liver damages. The efficacy of Spatholobi Caulis 30 mg/kg shows similar effects to that of silymarin 100 mg/kg, and more favorable hepatoprotective effects were observed in Spatholobi Caulis 50 mg/kg as compared with silymarin 100 mg/kg against Cd-intoxicated hepatopathies in the present study.

Keywords

References

  1. 전국한의과대학 본초학교수. 본초학. 서울:영림사. 1992:445-6.
  2. 鄭虎占. 中藥現代硏究與應用. 北京:學苑出版社. 1998:2539-46.
  3. 윤정선. 계혈등의 플라보노이드 성분[석사학위논문]. 서울: 서울대학교대학원: 2003.
  4. Zhu YP. Chinese materia medica. The Netherlands : Harwood academic publisher. 1998:468-70.
  5. Choi HS, Kim YC, Lee JS, Jo MR, Seo CH, Park SI. Antibacterial Activities of Hot-water and Ethyl Alcohol Extracts of Medicinal Herbs on Fish Pathogenic Bacteria. J. Fish Pathol. 2004; 17(1):39-55.
  6. Cha BC, Lee EH, Noh MA. Antioxidant Activity of Spatholobus suberectus Dunn. Kor. J. Phamacogn 2005;36(1):50-5.
  7. Sim GS, Kim JH, Lee DH, Park SM, Pyo HB, Zhang YH, et al. Effects of the Spatholobi calulis extract on Antioxidation and Inhibition of Matrix Metalloproteinase in Human Skin Fibroblasts. Korean J. Biotechnol. Bioeng. 2005;20(1):40-5.
  8. Lee SL, Choi CH, Baek JU, Youn DH, Jeong SH, Han U, et al. Experimental Study of Patholobi Caulis on the Transient Cerebral Ischemia in Rats. Korean J. Oriental Phyology & Pathology. 2007;21(5):1127-34.
  9. Choi SI, Park SJ, Byun SH, Lee JR, Park MK, Kim SC. Effects of Spatholobi Caulis MeOH Extract on the Production of NO and Pro-inflammatory Cytokines in LPS-activated Raw264.7 Cells. Korean J. Herbology. 2009;24(2):21-7.
  10. Choi JS, Song TW, Kim DH. A Study on the Effects of Spatholubus suberectus Dunn on the Inhibition of Arthritis Induced by Collagen on the Mouse. Korean J. Herbology. 2003;18(3): 79-99.
  11. Lee SL, Jeong HW. Experimental Effects of SPATHOLOBI CAULIS on the Cerebral Blood Flow and Lactate Dehydrogenase Activity. Korean J. Oriental Phyology & Pathology. 2006; 20(1):25-30.
  12. 이화경. 계혈등이 자궁근종세포의 증식억제와 세포자멸사 관련 유전자의 발현에 미치는 영향 [석사학위논문]. 대구: 대구한의대학교대학원: 2004.
  13. 김병회. 계혈등이 자궁경부암세포의 세포자멸사 유도와 관련유전자 발현에 미치는 영향[석사학 위논문]. 대구: 대구한의대학교대학원: 2005.
  14. 전용지. 계혈등이 자궁내막암세포의 증식억제 및 세포자멸사에 미치는 영향[석사학위논문]. 대구: 대구한의대학교대학원: 2005.
  15. Kim DC. Induction of cell cycle arrest and p21cip1/waf1 expression in human uterine leioma cells by isoliquiritigenin one of the chemical constituents of Spatholobus suberectus Dunn[박사학위논문]. 대구: 대구한의대학교대학원: 2005.
  16. 박선영. 계혈등의 Beta-sistosterol 성분이 자궁근 종세포 증식억제와 세포자멸사의 유도에 미치는 영향[석사학위논문]. 대구: 대구한의대학교대학원: 2005.
  17. Hwang SG, Lee HC, Kim CK. Kim DG, Song HJ, Park YJ, et al. Spatholobus suberectus Water Extract induces Apoptotic Cell Death via Inhibition of Cell Cycle in p53-Dificient Human Leukemia Cell Line Jurkat Korean J. Oriental Phyology & Pathology. 2001;15(6):887-92
  18. Wang W, Wang J, Zhao D, Liu H, Zhou W, Chen K. Comparison of Spatholobus suberectus Dunn Euonymus alatus (Thunb.) Sied. and Eupolyphage sinensis Walkeron regulation of plasma lipid. Zhongguo Zhoug Yao Za Zhi. 1991;16:299- 301,320.
  19. Buchet JP, Lauwerys R, Roels H, Bernard A, Bruaux P, Claeys F, et al. Renal effects of cadmium body burden of the general population. Lancet 1990;336:699-702. https://doi.org/10.1016/0140-6736(90)92201-R
  20. Hare L, Tessier A. Predicting animal cadmium concentrations in lakes. Nature 1996;380:430-2. https://doi.org/10.1038/380430a0
  21. Wittman R, Hu H. Cadmium exposure and nephropathy in a 28-year-old female metals worker. Environ Health Perspect. 2002;110:1261-6. https://doi.org/10.1289/ehp.021101261
  22. Patrick L. Toxic metals and antioxidants: part II.The role of antioxidants in arsenic and cadmium toxicity. Altern Med Rev. 2003;8:106-28.
  23. Swiergosz-Kowalewska R. Cadmium distribution and toxicity in tissues of small rodents. Microsc Res Tech. 2001;55:208-222. https://doi.org/10.1002/jemt.1171
  24. Theocharis S, Margeli A, Fasitsas C, Loizidou M, Deliconstantinos G. Acute exposure to cadmium causes time-dependent liver injury in rats. Comp Biochem Physiol C. 1991;99:127-30. https://doi.org/10.1016/0742-8413(91)90087-A
  25. Theocharis SE, Margeli AP, Giannakou N, Drakopoulos DS, Mykoniatis MG. Cadmiuminduced hepatotoxicity in three different rat strains. Toxicol Lett. 1994;70:39-48. https://doi.org/10.1016/0378-4274(94)90142-2
  26. Srivastava RK, Sharma S, Verma S, Arora B, Lal H. Influence of diabetes on liver injury induced by antitubercular drugs and on silymarin hepatoprotection in rats. Methods Find Exp Clin Pharmacol. 2008 Dec;30(10):731-7. https://doi.org/10.1358/mf.2008.30.10.1316824
  27. Habeebu SS, Liu J, Klaassen CD. Cadmiuminduced apoptosis in mouse liver. Toxicolgy and applied pharmacology. 1998;149;203-9. https://doi.org/10.1006/taap.1997.8334
  28. 김순호, 손한철, 이은엽, 장철훈. 최신임상검사진단학. 서울:계축문화사. 1996:150-7.
  29. 유승흠. 예방의학. 서울:계축문화사. 2004:520-2.
  30. Lee KJ, Kim JJ, Hwang JH, Kim KY. Background Exposure of Healthy Office Workers to Cadmium in Selected Korean Women. J. Occupational and Environmental Hygiene. 2008; 18(2)108-14.
  31. Page AL, Chang AC. Cadmium, Springer-Verlag. Berlin Heodelberg. 1986:33.
  32. Hwang YO, Kim SU, Ryu SH, Ham HJ, Park GY, Park SG. Contents of mercury, lead, cadmium, and arsenic in dried marine products. Analytical Science & Technology. 2009;22(4): 336-44.
  33. Moon CS. Evaluation of Cd and Pb Intake and Exposure Routes in Some Korean Women. Korean J. Env. Hlth. 2007;33(5):353-8. https://doi.org/10.5668/JEHS.2007.33.5.353
  34. Samarawickrama GP. Biological effects of cadmium in mammals, In Webb M(ed): "The chemistry, biochemistry and biology of cadmium." Amsterdam:Elsevier-North Holland Biomedical Press. 1979:341-422.
  35. Kunimoto M, Miysaka K, Miura T. Changes in membrane properties of rat blood cells induced by cadmium accumulating in the membrane fraction. J. Biochem. 1986;99:397-406. https://doi.org/10.1093/oxfordjournals.jbchem.a135494
  36. Manca D, Ricard AC, Trottier B, Chevalier G. Studies on lipid proxidation in rat tissues following administration of low and moderate doses of cadmium chloride. Toxicology. 1991;67 :303-23. https://doi.org/10.1016/0300-483X(91)90030-5
  37. Sarkar S, Yadav P, Trivedi R, Bansal AK, Bhatnagar D. Cadmium-induced lipid peroxi-dation and the status of the antioxidant system in rat tissues, J. Trace Element Med. Biol. 1995;9: 144-9. https://doi.org/10.1016/S0946-672X(11)80038-6
  38. Dalton T, Fu K, Enders GC, Palmiter RD, Andrews GK. Analysis of the effects of overexpression of metallothionein-I in transgenic mice on the reproductive toxicology of cadmium. Environ Health Persp. 1996;104:68-76. https://doi.org/10.1289/ehp.9610468
  39. Choi IH, Kim SO, Kim KS, Lee MY. Effect of Mungbean Sprouts Juice on Cadmium-Induced Hepatotoxicity in Rats. J. Korean Soc. Food Sci. Nutr. 1998;27(5):980-6.
  40. Lee IK, Kim JG. Effects of Extract of Eucommia ulmoides Oliv on the Reduction of Lead and Cadmium in Organs of Rats. J. KPHA. 2000; 26(1):22-8.
  41. Lee CH, Cho JK, Choe IS, Han KH, Kim CY. Effects of Mugwort - water Extracts on Cadmium Toxicity in Rats. Korean J. Food Science of Animal Products. 1999;19(2):188-97.
  42. Lee JH, Jeong SI, You IS, Kim SK, Lee KN, Han DS, et al. . The Inhibitory Effects of the Methanol Extract of Houttuynia cordata THUNB against Cadmium Induced Cytotoxicity (V). Kor. J. Pharmacogn. 2001;32(1):61-7.
  43. Lee JH, You IS, Kim SK, Lee KN, Chung WY, Han DS, et al. The Inhibitory Effects of Trichosanthes kirilowii Root against Cadmium Induced Cytotoxicity (III) Kor. J. Pharmacogn. 2001;32(1):15-21.
  44. Choi JH, Rhee SJ. Effects of Green Tea Catechin on Cadimium Accumulation in Chronic Cadmium Poisoned Rats. Korean J. Nutr. 2001;34(4):384- 92.
  45. Han SH, Shin MK, Chung YH. Effects of the Omija (Schizandra chinensis Baillon) Extract on the Metabolism and Renal Cadmium Contents in Cadmium Administered Rats. J. Korean Soc. Food Sci. Nutr. 2002;31(6):1102-6. https://doi.org/10.3746/jkfn.2002.31.6.1102
  46. Chung YH, Han SH, Shin MK. Effects of Pueraria radix in Water Extract on the Detoxification in Rat administered with Cadmium. KOREAN J. DIETARY CULTURE. 2002;17(4): 456-64.
  47. Yu IS, Lyu MH. A Study on the Effect of Chitin, Chitosan on the Cadmium Accumulation and the Enzyme Activity in Rat Kidney. EnvScience. 2002;11:55-60.
  48. Kim MJ, Hong JH, Lee SJ. Effect of Vitamin E on Cadmium Accumulation and Excretion in Chronic Cadmium Poisoned Rats. Korean J. Nutr. 2003;36(7):691-8.
  49. Shim JY, Cho YM, Kim YK, Lee HO, Om AS. Effects of soy isoflavones on detoxification of cadmium. Korean J. Soybean Society. 2005;22 (1):19-24.
  50. Kim TK, Park BH, Lee SI, Kim SD. Effects of Tea Beverages on Cadmium Accumulation and Excretion in Rats Given Cadmium. Korean Journal of Food Preservation . 2008;15(1):161-8.
  51. Rhim TJ. The Effects of Selenium on Cadmium- Induced Toxicity and Lipid Peroxidation in Rat Hepatocyte Primary Culture. Korean J. Society of Environmental Agriculture . 2003;22(2):94-9. https://doi.org/10.5338/KJEA.2003.22.2.094
  52. Kim SC, Byun SH, Yang CH, Kim CY, Kim JW,Kim SG. Cytoprotective effects of Glycyrrhizae radix extract and its active component liquiritigenin against cadmium-induced toxicity (effects on bad translocation and cytochrome c-mediated PARP cleavage). Toxicology. 2004;197(3):239-51. https://doi.org/10.1016/j.tox.2004.01.010
  53. Lee JR, Park SJ, Lee HS, Jee SY, Seo J, Kwon YK, et al. Hepatoprotective Activity of Licorice Water Extract against Cadmium-induced Toxicity in Rats. Evid Based Complement Alternat Med. 2009;6(2):195-201. https://doi.org/10.1093/ecam/nem078
  54. Park EY. Hepatoprotective and renoprotective effects of liquiritigenin, a component of licorice, on cadmium-induced toxicity in rats[석사학위논문]. 대구: 대구한의대학교 대학원: 2008.
  55. Pram TN, Marion M, Denizeau F, Jumarie C. Cadmium-induced apoptosis in rat hepatocytes does not necessarily involve caspase-dependent pathways. Toxicology in vitro. 2006;20:1331-42. https://doi.org/10.1016/j.tiv.2006.05.005
  56. Thophon S, Kruatrachue M, Upatham ES, Pokethitiyook P, Sahaphong S, Jaritkhuan S. Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environ Pollut. 2003;121(3):307-20. https://doi.org/10.1016/S0269-7491(02)00270-1
  57. Tzirogiannis KN, Panoutsopoulos GI, Demonakou MD, Hereti RI, Alexandropoulou KN, Mykoniatis MG. Effect of hepatic stimulator substance (HSS) on cadmium-induced acute hepatotoxicity in the rat liver. Dig Dis Sci. 2004;49(6):1019-28. https://doi.org/10.1023/B:DDAS.0000034566.43582.53
  58. Horiguchi H, Oguma E, Kayama F, Sato M, Fukushima M. Dexamethasone prevents acute cadmium-induced hepatic injury but exacerbates kidney dysfunction in rabbits. Toxicol Appl Pharmacol. 2001;174(3):225-34. https://doi.org/10.1006/taap.2001.9218
  59. Shibasaki T, Matsumoto H, Gomi H, Ohno I, Ishimoto F, Sakai O. Effects of a hepato-protective agent and a hepato-secreting chelator on cadmiuminduced nephrotoxicity in Syrian hamsters. Biol Trace Elem Res. 1996;52(1):1-9. https://doi.org/10.1007/BF02784085
  60. El-Ashmawy IM, Youssef SA. The antagonistic effect of chlorpromazine on cadmiumtoxicity.Toxicol Appl Pharmacol. 1999;161(1):34-9. https://doi.org/10.1006/taap.1999.8785
  61. Jeong YG, Choe MG, Park ST, Kim TY, Jeong YT. Toxic Effects of Cadmium(CdCl2) on the Liver and Heart in the Rat. EnvScience. 2003;12: 51-64.
  62. Sauer J-M, Waalkes MP, Hooser SB, Kuester RK, McQueen CA, Sipes IG. Suppression of Kupffer cell function prevents cadmium induced hepatocellular necrosis in the male Sprague- Dawley rat. Toxicology 1997;121:155-64. https://doi.org/10.1016/S0300-483X(97)00062-0
  63. Rej, R. Aspartate aminotransferase activity and isoenzyme proportions in human liver tissues. Clin Chem. 1978;24(11):1971-9.
  64. Sherlock S., Dooley J. Diseases of the Liver and Biliary System, 11th ed. London:Blackwell Science. 2002:23.