Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analyses for the Quality Evaluation of Salviae Miltiorrhizae Radix by HPLC

  • Fang, Zhe (College of Pharmacy, Catholic University of Daegu) ;
  • Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University) ;
  • Son, Kun-Ho (College of Life Science, Andong National University) ;
  • Son, Jong-Keun (College of Pharmacy, Yeungnam University) ;
  • Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu)
  • Received : 2010.08.10
  • Accepted : 2010.11.11
  • Published : 2010.12.31
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Abstract

In this study, quantitative analysis for the quality evaluation of Salviae Miltiorrhizae Radix using HPLC/UV was developed. For quantitative analysis, six major bioactive compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS $C_{18}$ column ($250{\times}4.6\;mm$, $5\;{\mu}m$) with gradient condition of A (1% formic acid in $H_2O$) and B (acetonitrile : methanol : formic acid = 100 : 75 : 1) as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 280 nm. These methods were fully validated with respect to the linearity, accuracy, precision and recovery. The HPLC/UV method was applied successfully to the quantification of six major compounds in the Salviae Miltiorrhizae Radix. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis.

Keywords

References

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