Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Generation of Antagonistic RNA Aptamers Specific to Proinflammatory Cytokine Interleukin-32

  • Kim, Se-Ho (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Jung-Hee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Yoon, Su-Jin (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Keun-Sik (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Yoon, Moon-Young (Department of Chemistry, Hanyang University) ;
  • Yoon, Do-Young (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University)
  • Received : 2009.09.09
  • Accepted : 2010.09.24
  • Published : 2010.12.20
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Abstract

Interleukin 32 (IL-32) is a recently identified cytokine that induces major proinflammatory cytokines such as $TNF{\alpha}$ and IL-$1{\beta}$, which play an important role in chronic inflammatory diseases. To antagonize the biological function of IL-32 in cells, we generated RNA aptamers that could bind specifically to IL-32 protein. The highest affinity aptamer, AC3-3, successfully antagonized IL-32 by abolishing the induction of $TNF{\alpha}$ in the human lung carcinoma cells expressing IL-32. This aptamer could be used as a potent and selective antagonist against IL-32 to further elucidate the roles of IL-32 in chronic inflammatory diseases, as well as a therapeutic agent.

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